DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells
Figure 2
Recovery of viable iPSCs after different protocols of cryopreservation. Freezing of iPSC 3 was performed using 3 basic protocols: STD: standard routine slow uncontrolled freezing of small clumps with 10% of CPA in Nunc cryovials, ACC: dissociation of the cells with Accutase and freezing in cryovials and freezing and re-plating in the presence of a ROCK Inhibitor Y-27632 (RI), PLT: Freezing of adherent cells in 4-well plates in EG. Four CPAs were used in STD and ACC experiments, which are abbreviated above. The cells in ACC and PLT were frozen and recovered after thawing in the presence of RI. The numbers on the last group indicates programmed freezing protocols described in Section 3.3 and in Figure 4. Fluorescent microphotographs of some of experimental variations are shown on Figures 3 and 4. , .
