DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells
Figure 3
Cryopreservation of human iPSCs in vials. Fluorescent microscopy of human induced pluripotent stem cells. Color combined, Nanog (immune staining), and Hoechst. The intensity of hPGK-H2B-eGFP was weak (not shown) but quantifiable by Cell Lab QUANTATM. The photos were taken on day 6 after thawing. Row STD: standard freezing with 10% of four different cryoprotective agents (CPAs) indicated in columns: dimethyl sulfoxide (DMSO) (current routine), ethylene glycol (EG), propylene glycol (PG), and glycerol (GLY). Row ACC: cryopreservation of human iPSCs after dissociation into single cells with Accutase and in the presence of ROCK inhibitor Y-27632 and different CPAs. Note: cryopreservation of dissociated by Accutase cells w/o RI resulted in 90% of cell death (Figure 2).
