SOX2 Is Regulated Differently from NANOG and OCT4 in Human Embryonic Stem Cells during Early Differentiation Initiated with Sodium Butyrate

Figure 4

Detection of pluripotency marker SOX2 and differentiation markers in differentiating H9 cells. Cells were treated and processed as described in the legend of Figure 2. Coexpression of pluripotency marker SOX2 and differentiation markers was detected by flow cytometry (a) or by Western blot (b). Results are representative of two independent experiments. (c) The number of cells in one well is shown as a mean value ± SEM of three experiments. (d) The changes in colony morphology detected at the beginning of differentiation (day 0) and on day 3.