Subarachnoid Hemorrhage, Spreading Depolarizations and Impaired Neurovascular Coupling

Figure 2

Increased amplitude of spontaneous Ca²⁺ oscillations in astrocyte endfeet following SAH. (a)-(b) Representative images of spontaneous Ca²⁺ oscillation in astrocyte endfeet in brain slices from control and SAH model animals. (b) Time laps images from the area within the yellow dotted box in Figure 2(a). Scale bar: 10 μm. (c) Spontaneous Ca²⁺ oscillations in a brain slice from control (upper) and SAH model (lower) animals. Traces were obtained from 1.2 × 1.2-μm regions of interest placed on distinct astrocyte endfeet in 5 min recordings without stimulation (modified from Koide et al. [21]).