Shiga Toxin-Producing Escherichia coli Isolated from Bovine Mastitic Milk: Serogroups, Virulence Factors, and Antibiotic Resistance Properties

Table 1

Primers used for detection of virulence genes in Shiga toxin-producing Escherichia coli isolated from bovine mastitis.

Virulence factor	Primers name	Primer sequences (5′-3′)	Product size (bp)	Reference
Shiga toxin 1 (stx1)	Stx1f	AAATCGCCATTCGTTGACTACTTCT	366	[20]
	Stx1r	TGCCATTCTGGCAACTCGCGATGCA
Shiga toxin 2 (stx2)	Stx2f	CGATCGTCACTCACTGGTTTCATCA	282	[20]
	Stx2r	GGATATTCTCCCCACTCTGACACC
Enteropathogenic attachment and effacement (eaeA)	EAE1	TGCGGCACAACAGGCGGCGA	629	[21]
	EAE2	CGGTCGCCGCACCAGGATTC
Haemolysin (ehly)	Hly F	CAATGCAGATGCAGATACCG	432	[22]
	Hly R	CAGAGATGTCGTTGCAGCAG