Shiga Toxin-Producing Escherichia coli Isolated from Bovine Mastitic Milk: Serogroups, Virulence Factors, and Antibiotic Resistance Properties

Table 3

Primers used for detection of antimicrobial resistant genes in Shiga toxin-producing Escherichia coli isolated from bovine mastitis.

Antibiotic	Resistant gene	Sequence	Size of product (bp)	Annealing temperature; (°C)	References
Streptomycin	aadA1	(F) TATCCAGCTAAGCGCGAACT	447	58	[29]
		(R) ATTTGCCGACTACCTTGGTC
Tetracycline	tetA	(F) GGTTCACTCGAACGACGTCA	577	57	[29]
		(R) CTGTCCGACAAGTTGCATGA
Tetracycline	tetB	(F) CCTCAGCTTCTCAACGCGTG	634	56	[29]
		(R) GCACCTTGCTGATGACTCTT
Trimethoprim	dfrA1	(F) GGAGTGCCAAAGGTGAACAGC	367	45	[30]
		(R) GAGGCGAAGTCTTGGGTAAAAAC
Fluoroquinolone	qnr	(F) GGGTATGGATATTATTGATAAAG	670	50	[31]
		(R) CTAATCCGGCAGCACTATTTA
Gentamicin	aac(3)-IV	(F) CTTCAGGATGGCAAGTTGGT	286	55	[32]
		(R) TCATCTCGTTCTCCGCTCAT
Sulfonamide	sul1	(F) TTCGGCATTCTGAATCTCAC	822	47	[32]
		(R) ATGATCTAACCCTCGGTCTC
Cephalothin	blaSHV	(F) TCGCCTGTGTATTATCTCCC	768	52	[32]
		(R) CGCAGATAAATCACCACAATG
Ampicillin	CITM	(F) TGGCCAGAACTGACAGGCAAA	462	47	[32]
		(R) TTTCTCCTGAACGTGGCTGGC
Chloramphenicol	cat1	(F) AGTTGCTCAATGTACCTATAACC	547	55	[32]
		(R) TTGTAATTCATTAAGCATTCTGCC
Chloramphenicol	cmlA	(F) CCGCCACGGTGTTGTTGTTATC	698	55	[32]
		(R) CACCTTGCCTGCCCATCATTAG