Anthelmintic Activity of Crude Extract and Essential Oil of Tanacetum vulgare (Asteraceae) against Adult Worms of Schistosoma mansoni

Figure 5

Quantitative analysis of morphological alterations on the tegument of S. mansoni after exposure to different concentrations of the crude hydroalcoholic extract of T. vulgare (TV). The quantification of the number of tubercles was performed using three-dimensional images obtained from laser scanning confocal microscopy. Indicated are numbers of intact tubercles, and these numbers were measured in a 20 000 μm² area in a dorsal region of Schistosoma mansoni adult male worm (see Figure 4(a)) and calculated with the Zeiss LSM Image Browser software. Praziquantel (PZQ, 10 μM) was used as a positive control. A minimum of three tegument areas of each parasite were assessed. Values are means ± SD (bars) of ten male adult worms. *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 compared with untreated group.