Table 1: Scheduled labs in Bio-251.

Week Scheduled labs 1 Basic laboratory safety techniques Basic equipment and bacteriological techniques 2 Basic calculations for preparing solutions of different concentrations 3 Bacterial media prep, inoculation, and growth 4 Isolation of chromosomal DNA and preparation of agarose gel 5 PCR and agarose gel analysis 6 Restriction enzyme digestion, purification, and preparation for cloning 7 Ligation 8 Transformation Making competent cells of E. coli and transforming the recombinant plasmid. 9 Isolation of Plasmid, gel electrophoresis, and screening for clone 10 Preparation for gene expression and transformation to a suitable strain for expression 11 Protein induction and expression 12 Polyacrylamide gel electrophoresis 13 Protein purification 14 Protein purification troubleshooting 15 Bioinformatics (genome analysis, Blast, Primer3 for designing PCR, Web-cutter) 16 Computer analysis of the gene product