Selective Sensing of Tyrosine Phosphorylation in Peptides Using Terbium(III) Complexes

Figure 9

Plots of the rates of EGFR kinase-catalyzed phosphorylation of P1 versus the concentrations of various inhibitors. The rates of phosphorylation of P1 by the kinase were determined in the presence of dasatinib (red), gefitinib (blue), imatinib (green), and staurosporine (black) by the method in Figure 8. The IC₅₀ values of the inhibitors, calculated by fitting these sigmoidal curves, are presented in Table 1, together with the values on Src and Fyn kinases. Reproduced with permission from [69]. Copyright 2014, Springer.