Proteomic and Bioinformatics Analyses of Mouse Liver Microsomes

Table 3

Proteins that are consistently present in both 2DE dataset of microsomal proteins (Table 1) and 1DE dataset of Na₂CO₃-extracted microsomal proteins (Table 2).

Accession number	Protein name	Predicted TMD	GRAVY scores	PI value	Location
IPI00108454	Similar to 40S ribosomal protein S6	0	−0.918	10.68	ER
IPI00112322^a	UDP-glucuronosyltransferase 2B5 precursor	1	−0.031	7.94	ER
IPI00129526	Endoplasmin precursor (ER protein 99, 94 kDa glucose-regulated protein)	0	−0.72	4.74	ER
IPI00133522	Protein disulfide-isomerase precursor	0	−0.386	4.79	ER
IPI00222496^a	Protein disulfide isomerase-associated 6	1	−0.292	5.05	ER
IPI00230108	Glucose-regulated protein, full insert sequence	0	−0.479	5.78	ER
IPI00319992	78 kDa glucose-regulated protein precursor	0	−0.481	5.07	ER
IPI00331322^a	Microsomal glutathione S-transferase 1	3	0.14	9.67	ER and mitochondrial outer membrane
IPI00319973^a	Membrane-associated progesterone receptor component 1	1	−0.616	4.57	ER, membrane bound
IPI00109061	Tubulin beta-4 chain homolog	0	−0.406	4.78	Cytoplasmic
IPI00110827	Actin, alpha skeletal muscle	0	−0.232	5.23	Cytoplasmic
IPI00110850	Actin, cytoplasmic 1	0	−0.2	5.29	Cytoplasmic
IPI00111908	Predicted: carbamoyl-phosphate synthetase 1	0	−0.12	6.42	Cytoplasmic
IPI00117348	Tubulin alpha-2 chain	0	−0.23	4.94	Cytoplasmic
IPI00117914	Arginase 1	0	−0.187	6.52	Cytoplasmic
IPI00134746	Argininosuccinate synthase	0	−0.361	8.36	Cytoplasmic
IPI00117978^a	Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor	1	−0.412	9.25	Mitochondrial inner membrane
IPI00315794^a	Cytochrome b5 outer mitochondrial membrane isoform precursor	1	−0.602	4.79	Mitochondrial outer membrane
IPI00122547^a	Voltage-dependent anion-selective channel protein 2	0	−0.223	7.44	Outer mitochondrial membrane
IPI00124790^a	Polyposis locus protein 1-like 1	3	0.375	6.82	Integral to membrane
IPI00138406^a	Ras-related protein Rap-1A	0	−0.375	6.39	Membrane
IPI00121788	Peroxiredoxin 1	0	−0.221	8.26	Microbody
IPI00131366	Keratin, type II cytoskeletal 6B	0	−0.488	8.32	Sarcolemma

^aMembrane proteins with one or more predicted trans-membrane origins or validated by references.