- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Reviewers Acknowledgment
- Submit a Manuscript
- Subscription Information
- Table of Contents
Volume 2010 (2010), Article ID 325183, 16 pages
Gene Expression Changes Induced by PPAR Gamma Agonists in Animal and Human Liver
1UMR INSERM U991, Faculté des Sciences Pharmaceutiques et Biologiques, 35043 Rennes, France
2Université de Rennes 1, 35065 Rennes, France
3Biologie Servier, 45520 Gidy, France
4Institut de Recherches Servier, 92150 Suresnes, France
5Institut de Recherches Servier, 92400 Courbevoie, France
Received 8 June 2010; Accepted 15 July 2010
Academic Editor: Barbara Abbott
Copyright © 2010 Alexandra Rogue et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Thiazolidinediones are a class of Peroxisome Proliferator Activated Receptor (PPAR) agonists that reduce insulin resistance in type 2 diabetic patients. Although no detectable hepatic toxicity has been evidenced in animal studies during preclinical trials, these molecules have nevertheless induced hepatic adverse effects in some treated patients. The mechanism(s) of hepatotoxicity remains equivocal. Several studies have been conducted using PCR analysis and microarray technology to identify possible target genes and here we review the data obtained from various in vivo and in vitro experimental models. Although PPAR is expressed at a much lower level in liver than in adipose tissue, PPAR agonists exert various PPAR-dependent effects in liver in addition to PPAR-independent effects. Differences in effects are dependent on the choice of agonist and experimental conditions in rodent animal studies and in rodent and human liver cell cultures. These effects are much more pronounced in obese and diabetic liver. Moreover, our own recent studies have shown major interindividual variability in the response of primary human hepatocyte populations to troglitazone treatment, supporting the occurrence of hepatotoxicity in only some individuals.
Obesity has emerged as a major health problem with 1.6 billion adults classified as overweight and obese. The condition is associated with type 2 diabetes, cardiovascular diseases, and several cancers  and is characterized by an increase in the size and number of adipocytes. Peroxisome proliferator-activated receptors (PPARs) act as lipid sensors and therefore represent critical molecular targets for the treatment of obesity. Thus, agonists of peroxisome proliferator-activated receptor (PPAR, also known as NR1C3) are used to treat non-insulin-dependent diabetes type 2. PPAR belongs to the superfamily of nuclear receptors; it acts as a critical transcription factor in the regulation of adipose differentiation, lipid storage, and of genes involved in energy storage and utilisation. One putative mechanism through which PPAR enhances insulin sensitivity is its ability to channel fatty acids into adipose tissue, thus decreasing plasma fatty acid concentration. PPAR can also affect insulin sensitivity by regulating hormones, cytokines, and proteins that are involved in insulin resistance . It exists as two forms encoded by multiple transcript variants. PPAR1 is the predominant isoform in humans; it is highly expressed in adipose tissue but is also expressed in many other cell types in which it plays important functions, particularly intestine and immune cells. PPAR1 is the main isoform found in liver. PPAR2 is found at high levels in different adipose tissues . Hepatic PPAR represents only 10–30% of the level in adipose tissue . The PPAR superfamily contains two other subtypes, PPAR (NR1C1) and PPAR (NR1C2). PPAR is highly expressed in liver, kidney, small intestine, heart, and muscle, and it involved in fatty acid catabolism. PPAR is ubiquitous; although less studied, it is also implicated in fatty acid oxidation .
The mechanisms of action of PPARs have been well studied. Following activation by their ligands and heterodimerisation with retinoid X receptor (RXR), PPARs undergo specific conformational changes that release corepressors (as NcoR2/SMRT) and allow for the recruitment of coactivators (as SRC1/NCoA1, TIF2/SRC2, CBP/P300, steroid receptor coactivator 1, RIP140 (receptor interacting protein 140), PPAR co-activator-1) [6–8]. PPARs then interact with the peroxisome proliferator element (PPRE) in the promoter region of their target genes involved in lipid catabolism, fatty acid transport, and glucose homeostasis . Their differential effects could be explained by the cell and promoter context as well as the availability of cofactors but also by the specific conformation changes of the receptor induced by each PPAR ligand that leads to differential promoter activation and chromatin remodelling of target genes .
A wide variety of natural and synthetic PPAR ligands have been identified. Besides natural ligands such as 15-deoxy-prostaglandin J2, a metabolite of prostaglandin D2 and vitamin E, PPAR agonists include several synthetic drug classes such as glitazones and tyrosine analogs. Thiazolidinediones (TZDs) are a class of PPAR agonists used in clinical practice to reduce plasma glucose level in type 2 diabetic patients. The adipose tissue is required for these agonists to exert their antidiabetic but not their lipidomic effects . TZDs of the first generation were found to be highly hepatotoxic; the first one, ciglitazone (CIG), was abandoned after clinical trials and the second, troglitazone (TRO), was rapidly withdrawn from the market after reports of severe liver failure and death . A second generation of PPAR agonists, rosiglitazone (ROSI) and pioglitazone (PIO), has been approved by the Food and Drug Administration (FDA) in 1999. Hepatic failures have also been observed after administration of these two TZDs but they were less frequent and severe . The antidiabetic activities of another class of PPAR agonists, referred as tyrosine analogs, such as GW1929 and GW7845, looked promising but none of these compounds has been released on the market as yet .
Since dual PPAR and PPAR agonists might provide broader beneficial metabolic effects through a simultaneous treatment of hyperglycemia and dyslipidemia, compounds targeting both PPAR and have been developed by the pharmaceutical industry. However, the first dual agonists, muraglitazar and tesaglitazar, have been stopped during clinical trials due to cardiac and renal side-effects, respectively . Other molecules are still under development, for example, drugs belonging to a new class called selective PPAR modulators (SPPARM) for the reduction of the side-effects found with glitazones, such as oedema and weight gain .
A major concern in the development of novel PPAR agonists that differ from the current therapeutics is their implication in tumor development in different tissues. Although, whether their activation promotes or limits this process remains unclear and may depend on specific conditions , the FDA requires 2-year carcinogenesis studies in rodents of new agonists prior to the commencement of clinical trials exceeding 6 months.
Major species differences exist in the sensitivity to TRO. During preclinical trials, TRO did not induce detectable hepatic toxicity in animals, including monkeys, which show similar metabolic profiles to humans , supporting the view that glitazone toxicity is restricted to human individuals having a particular phenotype. Consequently, it could be postulated that the use of human liver cell models represents a more suitable approach than the use of their animal counterparts for investigations of hepatotoxic effects of PPAR agonists.
Microarray technology represents a powerful tool to better understand the mechanisms of drug toxicity since it permits the identification of gene sets that are preferentially modulated after treatment. Several in vivo and in vitro studies have already been published on the effects of PPAR agonists on gene expression using different experimental conditions. However, they mainly concern PPAR agonists [18–22]. Studies on PPAR agonists are limited and are usually focused on nonhepatic tissues, especially adipose tissue. We review here the effects of PPAR agonists on hepatic gene expression described in the literature using either in vivo animal models or in vitro animal and human liver cell models and make comparison with our own recent data obtained with human hepatocyte cultures.
2. In Vivo Animal Studies
2.1. Effects of PPAR Agonists in Normal Liver
Little information exists on gene profiling changes induced by PPAR agonists in the liver of normal animals (Table 1); this might be explained by the low expression of this receptor in this organ. Most studies relate to PPAR agonist effects. However, a comparison of transcriptomic profiles of ROSI with six PPAR agonists has clearly shown that this glitazone does not significantly regulate any of the PPAR target genes in Sprague-Dawley rat liver . In this study, Cyp4a10, a cytochrome P450 involved in lipid metabolism, was induced 14-fold by Wy-14643 (one of the most potent PPAR agonists) and only 1.5-fold by ROSI. According to Memon et al. , the inability of TZDs to induce few PPAR-responsive genes, such as the carnitine palmitoyltransferase gene (Cpt-I), suggests that they may require the presence of other coactivators or may be under dominant regulatory control of other transcription factors. However, DeLuca et al.  demonstrated that TZDs induce acetyl CoA oxidase (Aco) and fatty acid binding protein 1 (Fabp1), which are known as PPAR target genes in both wild type and PPAR null mice, without any increase of PPAR expression. In addition, it should be noted that Brun et al.  have observed some degree of cross-activation between PPAR and PPAR with respect to the transcription of adipocyte differentiation genes, suggesting that residual PPAR receptor expressed in liver may be sufficient to mimicking PPAR function.
Although preclinical animal studies have not allowed the prediction of glitazone hepatotoxicity in humans, several studies in animals have dealt with the mechanisms of TZDs hepatotoxicity. A role of Cyp 3a1 has been advanced for the enhanced acetaminophen toxicity in rats when this compound is administered with TRO . However, chemical inhibition of drug metabolizing enzymes involved in TRO metabolism did not protect against TRO-induced toxicity. Another mechanism of toxicity could be the inhibition of the activity of the bile salt export pump (BSEP or ABCB11), which is responsible for cholestasis . However, TRO acts largely through the induction of apoptosis and the more likely mechanism is via effects on mitochondria resulting in the depletion of ATP and the release of cytochrome c . Interestingly, it has been recently shown that in a specific mouse model (whose mitochondrial antioxidant defense is slightly compromised), low repeated doses of TRO resulted in an oxidant injury to liver mitochondria, giving further support to mitochondria as targets for TRO-induced liver injury . Nevertheless, any of the effects could explain the initiating mechanism. TZDs also have anti-inflammatory properties. They inhibit macrophage activation and down-regulate proinflammatory cytokines such as tumor necrosis factor alpha (TNF-) and interleukin 6 (IL-6) in the liver of liposaccharide-stimulated mice [42, 43].
2.2. Effects of PPAR Agonists in Obese and Diabetic Liver
Several studies have dealt with the effects of PPAR agonists in obese and diabetic mouse liver. Enhanced levels of PPAR have been observed in the fatty liver of several animal models of obesity and diabetes, including ob/ob, db/db, A-Zip, and KKA mice [24, 29, 32]. Thus, KKA and ob/ob mice exhibit 8- and 6-fold more hepatic PPAR transcripts than C57BL/6 mice . This increase was more pronounced compared to that of PPAR mRNAs. On the contrary, Burant et al.  showed a 25% reduction in PPAR mRNA levels after TRO treatment of wild type animals.
The mechanistic relationship between steatosis and the increase of PPAR expression in the liver is still unclear. It is possible that elevated PPAR expression in ob/ob livers appears to be a pathophysiological response to the severity state of obesity and diabetes. In this regard, transcriptional effects of TZDs on their target genes have been shown to be exacerbated in obese diabetic versus lean control animals. Indeed, some genes were overexpressed in obese and diabetic mice compared to controls after treatment with TRO; for example, adipocyte fatty acid binding protein (Ap2 or Fabp4) and fatty acid translocase (Fat or Cd36) and others were increased only in the liver of ob/ob mice, for example, the uncoupling protein 2 gene (Ucp2) . The fact that PPAR target genes such as Cd36 were also induced by TRO treatment in lean mice without induction of PPAR expression  suggests that glitazone effects in obese or diabetic rodent models are different from those occurring in lean control animals. Therefore, it is essential to estimate glitazone effects in regard to the metabolic status of the animals. Noteworthy, the genes found to be modulated in the liver after glitazone treatment were unchanged in the adipose tissue .
Some differences have been observed among glitazone effects. Thus, only ROSI was found to induce an increase of both liver weight and hepatic triglycerides in AZIP/F1 mice. This could be explained by PPAR-independent mechanism effects . However, another explanation is the higher affinity of ROSI than TRO for the PPAR receptor. Indeed, ROSI caused higher incidence and severity of microvesicular steatosis in obese KKA mice compared to TRO due to its higher receptor affinity (approximately 100-fold) and its higher transcriptional response. In that study, the hepatic triglycerides content of treated and untreated animals was not different, leading to the conclusion that this microvesicular steatosis is not due to triglyceride accumulation. Exacerbation of fatty liver has also been reported with ROSI which exerted its effects on serum glucose levels independently of hepatic PPAR levels . Compared to wild type ob/ob mice, triglyceride content as well as mRNA levels of lipogenic genes, such as fatty acid synthase (Fasn), acetyl CoA carboxylase (Acc), and stearoyl CoA desaturase 1 (Scd1), were strongly decreased in corresponding PPAR-deficient animals . These data indicate that obese mice are more sensitive to the steatogenic effects of glitazones than lean animals.
It has been shown that a few PPAR target genes, such as Aco which is involved in peroxisomal -oxidation, were deregulated in diabetic rodent models after TZDs treatment. Since PPAR and PPAR recognize similar DNA response elements, it is quite conceivable that TZDs could modulate PPAR responsive genes in liver of obese mice . In wild type rodents, TRO and ROSI cause a decrease in serum cholesterol, triglycerides, free fatty acid content and, obviously, glucose levels without modifying liver or body weight [11, 23, 31]. Their effects on these biological parameters are substantially higher in obese mice.
To understand the effects of increased PPAR expression in fatty liver cells, Yu et al. used PPAR mice and explored gene effects of PPAR1 overexpression [27, 28]. Expression of genes involved in adipocyte differentiation and lipid metabolism was modulated in the liver of this KO mouse model. Noticeable increase was observed in Cd36, glucokinase (Gk), malic enzyme (Me), low-density lipoprotein (Ldl), microsomal transfer protein (Mtp), and angiopoietin-like 4 (Angptl4) in the absence of any change in CCAAT/enhancer binding protein alpha (C/ebp), sterol regulatory element binding protein 1 (Srebp1), phosphoenolpyruvate carboxykinase (Pepck), and glucose transporter type 2 (Glut-2 or Slc2a2) expression levels, leading to the conclusion that there is an adipogenic conversion of the liver when PPAR1 is overexpressed in this organ. Moreover, Vidal-Puig and coworkers demonstrated that forced expression of PPAR2 or 1 in fibroblasts was sufficient to drive the determination of an adipocyte cellular lineage . Furthermore, the relative abundance of PPAR in normal liver might serve as a key regulator of fatty acid catabolism, thereby minimizing the need for pathological adipogenic transformation of hepatocytes to store lipids. PPAR and fatty acid oxidation activity might partially protect from too high PPAR1 adipogenic activity in the liver. Way et al. analyzed transcript levels of genes involved in lipid and glucose homeostasis in Zucker Diabetic Fatty (ZDF) rats and concluded that PPAR activation had coordinate effects on genes involved in important hepatic metabolic pathways such as Pepck and glucose 6 phosphatase (G6P) which were decreased .
2.3. Extrahepatic Effects of PPAR Agonists
Marked tissue-differences are observed in the response to glitazones: relative to the liver and the skeletal muscle, PPAR is 10- to 30-fold higher expressed in human and rodent adipose tissues . Likewise, while PPAR agonists affect only a small number of genes in the liver and the skeletal muscle, they cause conspicuous changes in gene expression in adipose tissues . Thus, following a 14-day treatment of ZDF rats, ROSI decreased Tnf- and increased glucose transporter 4 (Glut4), muscle carnitine palmitoyl-transferase (Cat), stearoyl CoA desaturase (SCoA), and fatty acid translocase (Fat) in adipose tissue, while only Fat was slightly augmented in the liver which expresses very little hepatic PPAR. Comparison with the effects of retinoid X receptor-selective agonists, such as LG100268, that also produce insulin sensitization in diabetic rats, showed that these agonists modulated different gene patterns from those observed with ROSI, indicating that these compounds may act by independent and tissue-specific mechanisms .
Similar tissue-differences were observed in diabetic (db/db) mice treated with PIO for 2 weeks. Analysis of 42 genes associated with diabetes by RT q-PCR showed that in the liver, expression of Gk, Glut-2, apolipoprotein A-IV (ApoA-IV), PPAR, and a series of fatty acid oxidation enzymes were increased while those of triglyceride lipase, lipoprotein lipase, apolipoprotein A-I (Apo-AI), and insulin receptor substrate 2 (Irs-2) were decreased .
Glitazones decrease glucose concentrations not only by their action on adipocytes but also by their effects on the liver and muscle. Indeed in aP2/DTA mice, whose white and brown fat is virtually eliminated by fat-specific expression of diphterin toxin A chain, TRO alleviated hyperglycemia without affecting PPAR levels in liver, suggesting independence from both adipose tissue and PPAR receptor . However, conflicting observations have been reported. Thus, after mouse treatment with PIO, gene expression of Pepck was found to be increased in the liver by Hofmann et al.  but only in muscle by Suzuki et al. . Accordingly, increased expression of PPAR in the liver of diabetic mice has been reported in certain studies .
PPAR1 or 2 mRNA levels are not affected in adipose tissue by obesity in the ob/ob and Gold ThioGlucose (GTG) animal models. Accordingly, Auboeuf et al.  demonstrated that obesity and non-insulin-dependent diabetes mellitus are not associated with alteration in PPAR gene expression in adipose tissue in humans. However, conflicting observations have been made. Indeed, Vidal-Puig et al.  showed that expression of PPAR2 mRNA is increased in adipose tissue of obese men and women, and that the ratio of PPAR2/1 is directly correlated with their body mass index. In addition, they did not observe similar changes in muscle.
Besides its well-known function in adipocyte differentiation, PPAR activation by TZDs leads to an anti-inflammatory response in adipose tissue. This has been observed in fat deposits of various obese or diabetic rodent models  and in fat biopsies of type 2 diabetic patients . This anti-inflammatory response can be assessed by the inhibition of expression and/or biological activity of several proinflammatory factors such as TNF, IL-6, plasminogen activator inhibitor 1 (PAI-1), monocyte chemoattractant protein 1 (MCP-1), and angiotensinogen . Proposed molecular mechanisms, underlying this effect, include inhibition of the intracellular NF-kappaB pathway  and activation of nuclear translocation of the glucocorticoid receptor .
Macrophages accumulate in adipose tissue of obese animals, where they can produce inflammatory mediators, contributing by this way to insulin resistance . Targeted deletion of PPAR in macrophages severely impaired TZDs response in mice submitted to a high fat diet . These data emphasize the crucial role of macrophages for obtaining full effects of TZDs in the context of insulin resistance or in diabetic conditions.
Despite its weak expression level, PPAR is thought to play a role as a regulator of insulin action in the skeletal muscle . Indeed, it has recently been shown that muscle-specific PPAR deletion in mouse caused insulin resistance . PIO treatment of a murine model of myoblasts, the C2C12 cells, improved insulin sensitivity as assessed by increased glucose uptake . Moreover, some data indicate that PPAR activation in skeletal muscle could contribute to the beneficial effect of TZDs. Indeed, experiments on myocyte models have shown that ROSI induced local expression of the insulin-sensitizing hormone adiponectin . However, conflicting results on the role of PPAR in muscle have also been published, showing that muscle specific PPAR KO did not impair TZD action in a mouse model of insulin resistance . These results raise the question of which tissues are really necessary to achieve pharmacological action of TZDs. A precise analysis of PPAR regulation in other tissues where its expression reaches a sufficient level could lead to an answer.
3. In Vitro Animal Studies
Cytotoxicity studies have shown that primary rat hepatocytes were not more sensitive to TZDs than cells that did not express the drug metabolizing enzymes involved in their metabolism. TRO was more toxic than ROSI and PIO at equimolar concentrations . TRO induced a decline in mitochondrial transmembrane potential and apoptosis as well as an oxidative stress. These effects were also observed in other cell types and on isolated mitochondria .
Several studies have been carried out on modulation of gene expression by TZDs in rodent hepatocytes (Table 1). Different concentrations and exposure times have been tested although a 24h-treatment was the most frequent. Using Applied Biosystem rat genome survey microarrays with 26857 probes, Guo et al.  compared the effects of five PPAR agonists, including TRO, CIG, ROSI, and PIO, in rat hepatocytes after a 6-hour treatment. Around 2-fold more genes were modulated in TRO- and CIG- than in ROSI- and PIO-treated cell samples. Genes related to cell death were deregulated only with the most cytotoxic TRO and CIG concentrations. Similar observations were reported by Vansant et al. . TRO was also found to modulate more genes than other glitazones (ROSI, PIO) at the same concentration, especially genes related to oxidative stress, DNA repair, and cell death, such as heme oxygenase 1 (Ho-1), NAD(P)H quinone oxidoreductase (Nqo), growth arrest DNA-damage-inducible 45 (Gadd45), FBJ osteosarcoma oncogene (Fos), BCL2-like 11 (Bcl2l11), and BH3 interacting domain 3 (Bid3). A TRO response closer to CIG than to the second TZDs generation, ROSI and PIO, was also found in the C9 rat liver cell line .
As observed in vivo , TRO induced expression of the PPAR gene  and repressed genes related to lipid metabolism, such as Fasn and Cebp/, in cultured rat hepatocytes . Cyp induction by TZDs was evidenced in cultured primary rat hepatocytes using RTq-PCR analyses. Thus Cyp 3a and 2b subfamily genes were increased after exposure to TRO, ROSI and PIO [35, 36, 65]. Other genes including multidrug resistance (Mdr) 2 and 3, cadherin and superoxide dismutase (Sod) 2, were also up-regulated while Mdr1 and organic anion transporting polypeptide 8 (Oatp 8) were down-regulated .
PPAR2 expression was shown to induce lipid accumulation in the mouse AML12 liver cell line stably expressing PPAR2, and several genes known to be overexpressed in steatotic liver of ob/ob mice were found to be up-regulated by TRO, such as adipose differentiation-related protein (Adrp), Fabp4, Srebp1, Fasn, and Acc by q-PCR analysis. Lipid accumulation and the lipid droplet protein were further increased after a 7-day treatment with TRO .
An extensive study of gene expression changes induced by TZDs has also been performed on the mouse 3T3-L1 adipocyte cells using microarrays and RTq-PCR . Expression gene profiles obtained with TRO, ROSI and PIO tested at concentrations that elicited maximum biological effects (i.e., 20 M for PIO and TRO and 1 M for ROSI) were distinct but with an overlapping: 94 out of the 326 deregulated genes were found to be modulated by the three glitazones after a 24-hour treatment. For example, pepck, pyruvate dehydrogenase kinase 4 (Pdk4) and c-Cbl-associated protein (Cap) were activated by the three compounds but with different time-curves, suggesting different mechanisms of gene regulation. Moreover, ROSI and PIO were more potent than TRO in activating Pepck and Pdk4 and repressing regulator of G-protein signaling 2 (Rgs2). These data support the conclusion that gene profile changes induced by TZDs are different in liver cells and in adipocytes, in agreement with in vivo observations.
4. In Vitro Human Liver Cell Studies
Most studies on the effects of TZDs in human liver have been performed with primary hepatocyte cultures or hepatoma cell lines. Primary human hepatocytes are recognized as the most pertinent in vitro model but they exhibit early phenotypic alterations and their survival does not exceed a few days in standard culture conditions. Human hepatocytes have, in addition, a scarce and unpredictable availability and are characterized by large interdonor functional variability . Hepatic cell lines were thought to be an alternative but most of them have lost most of, if not all, their bioactivation capacity and consequently are of limited interest. In this regard, the new human hepatoma HepaRG cell line seems as an exception . HepaRG cells exhibit a capacity of transdifferentiation; they undergo morphological and functional features of liver bipotent progenitor cells after plating and at subconfluence, lose expression of progenitor markers and differentiate into either hepatocyte-like or cholangiocyte-like cells . Differentiated HepaRG cells possess most functional activities of primary mature hepatocytes and the indefinite growth capacity of hepatoma cells . Noteworthy, they express major cytochromes P450, conjugating enzymes, and plasma transporters .
The mechanism(s) of TZD hepatotoxicity in humans still remain(s) controversial. Several proposals have been advanced to explain the induction of apoptosis by TRO namely accumulation of toxic metabolites or bile acids, mitochondrial damage, and oxidative stress. TRO has been shown to be metabolized by CYP3A4 (the homolog of Cyp3a1 in rodent) to a very active quinone metabolite which is able to produce reactive oxygen species via the redox/cycling or to bind to cellular proteins . This CYP3A4-mediated metabolism is in accordance with the frequent occurrence of centrilobular necrosis of the liver. CYP3A4 is also induced by TRO in primary human hepatocytes [71, 72] and a correlation has been observed between CYP3A4 levels and hepatocyte sensitivity to glitazone .
Studies dealing with the effects of TZDs on gene expression in either primary human hepatocyte cultures or human hepatoma cell lines are scarce and most of them studied only few genes [76, 79] (Table 2). Human hepatocytes were slightly more sensitive than their rat counterparts , but less sensitive than human hepatoma HepG2 cells to cytotoxicity induced by TRO, supporting the conclusion of an absence of correlation between TRO toxicity and its hepatic metabolism. TRO was found to induce cell arrest and to cause time- and concentration-dependent apoptosis in various liver cell types [75, 77, 83]. Cell arrest was associated with increased expression of a cascade of cyclin-dependent kinase inhibitors, that is, cdki p21, p27, and p18 that each plays a crucial role in adipocyte differentiation through PPAR activation . This increase occurred through down-regulation of nuclear S-phase kinase-associated protein 2 (SKP2) . Apoptosis was associated with activation of both c-Jun N-terminal protein kinase and p38 kinase and overexpression of proapoptotic proteins and cyclooxygenase 2 (COX-2) [77, 83]. These effects are not limited to hepatoma cell lines. Indeed, TRO also induced growth arrest of prostate and bladder carcinoma cell lines . By contrast, other members of the glitazone family, ROSI or PIO, had no effect on the growth of these cell lines  and did not cause any apoptosis of HepG2 cells . The endogenous ligand 15-deoxy-prostaglandin J2 was also found to inhibit growth of prostate and bladder carcinoma cell lines by inducing apoptosis . Since these effects are selective of the PPAR ligand and the cell line, they can be interpreted as PPAR-independent effects . Apoptosis induced by TRO in human MCF-7 breast carcinoma cells has been associated with induction of GADD45 gene expression  while growth inhibition has been correlated to overexpression of another DNA damage gene, GADD153, in nonsmall lung carcinoma cells .
As observed in rat hepatocytes, Kier and coworkers  showed that TRO induced more genes than ROSI in human hepatocytes. This observation was based on analysis of gene expression profiles and did not include individual characterization of deregulated genes. Other studies have shown a down-regulation of SREBP-2, a gene encoding the sterol regulatory element-binding protein-2 that mediates cholesterol synthesis, as well as the two SREBP-2 target genes, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGCR) and low density lipoprotein receptor (LDLR), in HepG2 cells exposed to 30 M TRO for 4 h . In agreement with in vivo human data, TZDs were also found to modulate CYP activities in human hepatocyte cultures. CYP3A4 and CYP2B6 were induced by TRO  and only CYP2B6 by ROSI and PIO  in primary human hepatocytes. Similar observations were made in the well-differentiated HepaRG cell line .
Up to now, studies on human hepatocytes have been limited to a few donors (one to three), and no interdonor variability has been considered. Since interdonor variability in response to chemical inducers or inhibitors is well established, we recently compared the effects of TRO in human hepatocyte cultures from five donors after a 24-hour treatment using pangenomic microarrays (Rogue et al., unpublished data). Two-dimensional hierarchical clustering of gene expression profiles showed that hepatocyte populations separated according to the donor and not to the TRO concentration (Figure 1). It exhibited two separate clusters: one with donors 4 and 5 and the second with donors 1, 2, and 3. The number of genes modulated by TRO greatly varied as a function of the donor and drug concentration. At 5 and 20 M, TRO modulated 5754 and 7266 genes, respectively, in at least one donor but only 4 and 29 genes in the 5 donors, respectively (Figure 2). The small subset of common deregulated genes in hepatocyte cultures from several donors is in agreement with the findings reported by Goyak et al. , showing that the number of modulated genes deregulated in ten populations of human hepatocytes by arochlor 1254, di(2-ethylhexyl)phthalate, and phenobarbital did not exceed 0.1%. In our study, among the few genes deregulated in the five donors by 5 M TRO, only two genes involved in oxidative stress, namely, mannose binding lectin 2 (MBL2) and serum/glucocorticoid regulated kinase 2 (SGK2), were induced. Genes involved in lipid metabolism, such as FABP1 were deregulated only by 20 M TRO in all the donors. Several PPAR target genes, such as CYP4A1, CPT1, or CD36, were induced in the two hepatocyte cultures treated by 40 M TRO.
Despite its therapeutic indications, TRO only slightly affected transcription of genes involved in glucose homeostasis. Fructose-1,6-bisphosphatase 1 (FBP1), an enzyme involved in gluconeogenesis, was not modulated in any of the five donors, in agreement with previous observations  while PDK4 and PEPCK were differently regulated across the donors. Their transcription could be either induced, repressed, or not affected by TRO treatment.
Comparison of gene profiles after TRO treatment in five human hepatocyte populations and the well-differentiated human hepatoma HepaRG cells evidenced a clear separation between the two cell models by two-dimensional hierarchical clustering. HepaRG cell samples separated as a function of TRO concentration and the dendrogram showed that they were closer to donors 4 and 5 than to donors 1, 2, and 3. The number of commonly modulated genes between HepaRG cells and primary human hepatocytes increased with the drug concentration; it was higher than the number of commonly modulated genes in four out of five donors. Among them, genes involved in lipid metabolism, such as FABP4 or CD36, were induced. Taken altogether our data support the view that the effects induced by TRO and more generally by PPAR agonists are quite variable from one individual to another. This could explain the occurrence of toxic effects in only few treated patients. However, whether it would be possible to predict their potential hepatotoxicity in some patients on the basis of analysis of the expression level of a peculiar gene subset requires further studies.
Despite the numerous published studies on TZDs, their pharmacological and toxicological effects still remain obscure. Adipose tissue seems to be a predominant target organ. However, achievement of TZD pharmacological efficiency is obtained not only through an adipose-mediated mechanism but also requires an action in other organs, notably liver and skeletal muscle and also, as recently reported, in macrophages . Moreover, PPAR itself is required in a majority of metabolic tissues for regulation of insulin action and normal physiologic response to nutrients and it plays a critical role in the development of steatosis.
The determinants of susceptibility to glitazone-induced idiosyncratic hepatotoxicity remain to be elucidated. Studies have mainly concerned the most cytotoxic compound TRO. Evidence has been provided that toxicity is not directly related to its metabolism and the generation of a quinone metabolite. Direct toxicity caused by mitochondrial dysfunction has been demonstrated using both hepatic and nonhepatic in vitro models. Whether hepatotoxic effects of TZDs are related to PPAR activation is not clear. PPAR is only poorly expressed in the liver and both dependent and independent effects of glitazones have been seen.
Most in vitro studies have been performed with TRO concentrations of 50 M or more while maximum plasma concentrations reached 3 to 6 M in humans, making questionable the extrapolation of in vitro data to the in vivo situation. In comparison to TRO, ROSI is less toxic. The daily dose necessary for TRO therapeutic efficacy was 200 to 600 mg/day while it is only 4 to 8 mg/day for ROSI, indicating that patients were exposed to quite different doses between the first and second generations of TZDs .
During the last years, some studies have been designed to identify potential hepatic target genes in in vivo and in vitro models using RT-qPCR and microarray technologies. The amount of available data is still limited and has been obtained from different experimental conditions. However, some genes have been found to be usually modulated, being mainly related to drug and lipid metabolism. Interestingly, we have observed massive interindividual variability in the response of primary human hepatocytes to TRO treatment that could reflect the human situation. However, much more work is needed in order to identify the more pertinent genes which are differently expressed in the human hepatocyte populations and to determine whether the most critical effects in the liver are dependent or not on PPAR activation. Moreover, it would be important to estimate the effects resulting from long-term repeated glitazone treatments and to determine if intracellular PPAR levels in human liver cells are a critical parameter. The possible long-term treatments of differentiated normal and steatotic human hepatoma HepaRG cells could represent a unique way to better understand hepatotoxicity of PPAR agonists.
Alexandra Rogue was a recipient of a CIFRE contract. Personal studies have been supported by Servier Group and the EEC contract Predict IV, number 20222. The authors thank the Biological Resources Centre of Rennes and Biopredic International for the supply of isolated human hepatocytes. They also wish to thank Dr. Wynne Ellis and Dr. Delphine Allorge for careful reading of the manuscript.
- S. Low, M. C. Chin, and M. Deurenberg-Yap, “Review on epidemic of obesity,” Annals of the Academy of Medicine Singapore, vol. 38, no. 1, pp. 57–59, 2009.
- B. Staels and J.-C. Fruchart, “Therapeutic roles of peroxisome proliferator-activated receptor agonists,” Diabetes, vol. 54, no. 8, pp. 2460–2470, 2005.
- L. Michalik, J. Auwerx, J. P. Berger et al., “International union of pharmacology. LXI. Peroxisome proliferator-activated receptors,” Pharmacological Reviews, vol. 58, no. 4, pp. 726–741, 2006.
- L. Fajas, D. Auboeuf, E. Raspé et al., “The organization, promoter analysis, and expression of the human PPARγ gene,” Journal of Biological Chemistry, vol. 272, no. 30, pp. 18779–18789, 1997.
- T. Tanaka, J. Yamamoto, S. Iwasaki et al., “Activation of peroxisome proliferator-activated receptor δ induces fatty acid β-oxidation in skeletal muscle and attenuates metabolic syndrome,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 26, pp. 15924–15929, 2003.
- C. Yu, K. Markan, K. A. Temple, D. Deplewski, M. J. Brady, and R. N. Cohen, “The nuclear receptor corepressors NCoR and SMRT decrease peroxisome proliferator-activated receptor γ transcriptional activity and repress 3T3-L1 adipogenesis,” Journal of Biological Chemistry, vol. 280, no. 14, pp. 13600–13605, 2005.
- J. N. Feige, L. Gelman, L. Michalik, B. Desvergne, and W. Wahli, “From molecular action to physiological outputs: peroxisome proliferator-activated receptors are nuclear receptors at the crossroads of key cellular functions,” Progress in Lipid Research, vol. 45, no. 2, pp. 120–159, 2006.
- C. Grommes, G. E. Landreth, and M. T. Heneka, “Antineoplastic effects of peroxisome proliferator-activated receptor γ agonists,” Lancet Oncology, vol. 5, no. 7, pp. 419–429, 2004.
- D. D. Sears, A. Hsiao, J. M. Ofrecio, J. Chapman, W. He, and J. M. Olefsky, “Selective modulation of promoter recruitment and transcriptional activity of PPARγ,” Biochemical and Biophysical Research Communications, vol. 364, no. 3, pp. 515–521, 2007.
- J. M. Olefsky, “Treatment of insulin resistance with peroxisome proliferator-activated receptor γ agonists,” Journal of Clinical Investigation, vol. 106, no. 4, pp. 467–472, 2000.
- L. Chao, B. Marcus-Samuels, M. M. Mason et al., “Adipose tissue is required for the antidiabetic, but not for the hypolipidemic, effect of thiazolidinediones,” Journal of Clinical Investigation, vol. 106, no. 10, pp. 1221–1228, 2000.
- L. Guo, L. Zhang, Y. Sun et al., “Differences in hepatotoxicity and gene expression profiles by anti-diabetic PPAR γ agonists on rat primary hepatocytes and human HepG2 cells,” Molecular Diversity, vol. 10, no. 3, pp. 349–360, 2006.
- K. K. Brown, B. R. Henke, S. G. Blanchard et al., “A novel N-aryl tyrosine activator of peroxisome proliferator-activated receptor-γ reverses the diabetic phenotype of the Zucker diabetic fatty rat,” Diabetes, vol. 48, no. 7, pp. 1415–1424, 1999.
- C. Fiévet, J.-C. Fruchart, and B. Staels, “PPARα and PPARγ dual agonists for the treatment of type 2 diabetes and the metabolic syndrome,” Current Opinion in Pharmacology, vol. 6, no. 6, pp. 606–614, 2006.
- L. S. Higgins and A. M. Depaoli, “Selective peroxisome proliferator-activated receptor γ (PPARγ) modulation as a strategy for safer therapeutic PPARγ activation,” American Journal of Clinical Nutrition, vol. 91, no. 1, pp. 267S–272S, 2010.
- L. Michalik, B. Desvergne, and W. Wahli, “Peroxisome-proliferator-activated receptors and cancers: complex stories,” Nature Reviews Cancer, vol. 4, no. 1, pp. 61–70, 2004.
- P. B. Watkins and R. W. Whitcomb, “Hepatic dysfunction associated with troglitazone,” New England Journal of Medicine, vol. 338, no. 13, pp. 916–917, 1998.
- S. P. Anderson, C. Dunn, A. Laughter et al., “Overlapping transcriptional programs regulated by the nuclear receptors peroxisome proliferator-activated receptor α, retinoid X receptor, and liver X receptor in mouse liver,” Molecular Pharmacology, vol. 66, no. 6, pp. 1440–1452, 2004.
- L. Guo, H. Fang, J. Collins et al., “Differential gene expression in mouse primary hepatocytes exposed to the peroxisome proliferator-activated receptor α agonists,” BMC Bioinformatics, vol. 7, supplement 2, article S18, 2006.
- H. K. Hamadeh, P. R. Bushel, S. Jayadev et al., “Gene expression analysis reveals chemical-specific profiles,” Toxicological Sciences, vol. 67, no. 2, pp. 219–231, 2002.
- M. Rakhshandehroo, G. Hooiveld, M. Müller, and S. Kersten, “Comparative analysis of gene regulation by the transcription factor PPARα between mouse and human,” PLoS ONE, vol. 4, no. 8, article e6796, 2009.
- L. Richert, C. Lamboley, C. Viollon-Abadie et al., “Effects of clofibric acid on mRNA expression profiles in primary cultures of rat, mouse and human hepatocytes,” Toxicology and Applied Pharmacology, vol. 191, no. 2, pp. 130–146, 2003.
- K. S. Frederiksen, E. M. Wulff, P. Sauerberg, J. P. Mogensen, L. Jeppesen, and J. Fleckner, “Prediction of PPAR-α ligand-mediated physiological changes using gene expression profiles,” Journal of Lipid Research, vol. 45, no. 3, pp. 592–601, 2004.
- R. A. Memon, L. H. Tecott, K. Nonogaki et al., “Up-regulation of peroxisome proliferator-activated receptors (PPAR-α) and PPAR-γ messenger ribonucleic acid expression in the liver in murine obesity: troglitazone induces expression of PPAR-γ-responsive adipose tissue-specific genes in the liver of obese diabetic mice,” Endocrinology, vol. 141, no. 11, pp. 4021–4031, 2000.
- J. G. DeLuca, T. W. Doebber, L. J. Kelly et al., “Evidence for peroxisome proliferator-activated receptor (PPAR)α-independent peroxisome proliferation: effects of PPARγ/δ-specific agonists in PPARα-null mice,” Molecular Pharmacology, vol. 58, no. 3, pp. 470–476, 2000.
- R. P. Brun, P. Tontonoz, B. M. Forman et al., “Differential activation of adipogenesis by multiple PPAR isoforms,” Genes and Development, vol. 10, no. 8, pp. 974–984, 1996.
- S. Yu, K. Matsusue, P. Kashireddy et al., “Adipocyte-specific gene expression and adipogenic steatosis in the mouse liver due to peroxisome proliferator-activated receptor γ1 (PPARγ1) overexpression,” Journal of Biological Chemistry, vol. 278, no. 1, pp. 498–505, 2003.
- S. Yu, N. Viswakarma, S. K. Batra, M. Sambasiva Rao, and J. K. Reddy, “Identification of promethin and PGLP as two novel up-regulated genes in PPARγ1-induced adipogenic mouse liver,” Biochimie, vol. 86, no. 11, pp. 743–761, 2004.
- O. Gavrilova, M. Haluzik, K. Matsusue et al., “Liver peroxisome proliferator-activated receptor γ contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass,” Journal of Biological Chemistry, vol. 278, no. 36, pp. 34268–34276, 2003.
- J. M. Way, W. W. Harrington, K. K. Brown et al., “Comprehensive messenger ribonucleic acid profiling reveals that peroxisome proliferator-activated receptor γ activation has coordinate effects on gene expression in multiple insulin-sensitive tissues,” Endocrinology, vol. 142, no. 3, pp. 1269–1277, 2001.
- C. F. Burant, S. Sreenan, K.-I. Hirano et al., “Troglitazone action is independent of adipose tissue,” Journal of Clinical Investigation, vol. 100, no. 11, pp. 2900–2908, 1997.
- M. Bedoucha, E. Atzpodien, and U. A. Boelsterli, “Diabetic KKAy mice exhibit increased hepatic PPARγ1 gene expression and develop hepatic steatosis upon chronic treatment with antidiabetic thiazolidinediones,” Journal of Hepatology, vol. 35, no. 1, pp. 17–23, 2001.
- G. F. Davies, R. L. Khandelwal, and W. J. Roesler, “Troglitazone induces expression of PPARγ in liver,” Molecular Cell Biology Research Communications, vol. 2, no. 3, pp. 202–208, 1999.
- B. Lauer, G. Tuschl, M. Kling, and S. O. Mueller, “Species-specific toxicity of diclofenac and troglitazone in primary human and rat hepatocytes,” Chemico-Biological Interactions, vol. 179, no. 1, pp. 17–24, 2009.
- J. Sahi, G. Hamilton, M. Sinz et al., “Effect of troglitazone on cytochrome P450 enzymes in primary cultures of human and rat hepatocytes,” Xenobiotica, vol. 30, no. 3, pp. 273–284, 2000.
- G. Vansant, P. Pezzoli, R. Saiz et al., “Gene expression analysis of troglitazone reveals its impact on multiple pathways in cell culture: a case for in vitro platforms combined with gene expression analysis for early (idiosyncratic) toxicity screening,” International Journal of Toxicology, vol. 25, no. 2, pp. 85–94, 2006.
- S. E. Schadinger, N. L. R. Bucher, B. M. Schreiber, and S. R. Farmer, “PPARγ2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes,” American Journal of Physiology, vol. 288, no. 6, pp. E1195–E1205, 2005.
- J. Li, T. Kaneko, Y. Wang, L.-Q. Qin, P.-Y. Wang, and A. Sato, “Troglitazone enhances the hepatotoxicity of acetaminophen by inducing CYP3A in rats,” Toxicology, vol. 176, no. 1-2, pp. 91–100, 2002.
- C. Funk, C. Ponelle, G. Scheuermann, and M. Pantze, “Cholestatic potential of troglitazone as a possible factor contributing to troglitazone-induced hepatotoxicity: in vivo and in vitro interaction at the canalicular bile salt export pump (Bsep) in the rat,” Molecular Pharmacology, vol. 59, no. 3, pp. 627–635, 2001.
- M. T. Smith, “Mechanisms of troglitazone hepatotoxicity,” Chemical Research in Toxicology, vol. 16, no. 6, pp. 679–687, 2003.
- Y. H. Lee, M. C. M. Chung, Q. Lin, and U. A. Boelsterli, “Troglitazone-induced hepatic mitochondrial proteome expression dynamics in heterozygous mice: two-stage oxidative injury,” Toxicology and Applied Pharmacology, vol. 231, no. 1, pp. 43–51, 2008.
- S. Sigrist, M. Bedoucha, and U. A. Boelsterli, “Down-regulation by troglitazone of hepatic tumor necrosis factor-α and interleukin-6 mRNA expression in a murine model of non-insulin-dependent diabetes,” Biochemical Pharmacology, vol. 60, no. 1, pp. 67–75, 2000.
- M. Ricote, A. C. Li, T. M. Willson, C. J. Kelly, and C. K. Glass, “The peroxisome proliferator-activated receptor-γ is a negative regulator of macrophage activation,” Nature, vol. 391, no. 6662, pp. 79–82, 1998.
- K. Matsusue, M. Haluzik, G. Lambert et al., “Liver-specific disruption of PPARγ in leptin-deficient mice improves fatty liver but aggravates diabetic phenotypes,” Journal of Clinical Investigation, vol. 111, no. 5, pp. 737–747, 2003.
- U. Edvardsson, M. Bergström, M. Alexandersson, K. Bamberg, B. Ljung, and B. Dahllöf, “Rosiglitazone (BRL49653), a PPARγ-selective agonist, causes peroxisome proliferator-like liver effects in obese mice,” Journal of Lipid Research, vol. 40, no. 7, pp. 1177–1184, 1999.
- A. Vidal-Puig, M. Jimenez-Liñan, B. B. Lowell et al., “Regulation of PPAR γ gene expression by nutrition and obesity in rodents,” Journal of Clinical Investigation, vol. 97, no. 11, pp. 2553–2561, 1996.
- B. M. Spiegelman, “PPAR-γ: adipogenic regulator and thiazolidinedione receptor,” Diabetes, vol. 47, no. 4, pp. 507–514, 1998.
- H. Singh Ahuja, S. Liu, D. L. Crombie et al., “Differential effects of rexinoids and thiazolidinediones on metabolic gene expression in diabetic rodents,” Molecular Pharmacology, vol. 59, no. 4, pp. 765–773, 2001.
- A. Suzuki, T. Yasuno, H. Kojo, J. Hirosumi, S. Mutoh, and Y. Notsu, “Alteration in expression profiles of a series of diabetes-related genes in db/db mice following treatment with thiazolidinediones,” Japanese Journal of Pharmacology, vol. 84, no. 2, pp. 113–123, 2000.
- C. Hofmann, K. Lorenz, D. Williams, B. J. Palazuk, and J. R. Colca, “Insulin sensitization in diabetic rat liver by an antihyperglycemic agent,” Metabolism, vol. 44, no. 3, pp. 384–389, 1995.
- D. Auboeuf, J. Rieusset, L. Fajas et al., “Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-α in humans: no alteration in adipose tissue of obese and NIDDM patients,” Diabetes, vol. 46, no. 8, pp. 1319–1327, 1997.
- M. Abdelrahman, A. Sivarajah, and C. Thiemermann, “Beneficial effects of PPAR-γ ligands in ischemia-reperfusion injury, inflammation and shock,” Cardiovascular Research, vol. 65, no. 4, pp. 772–781, 2005.
- M. Kolak, H. Yki-Järvinen, K. Kannisto et al., “Effects of chronic rosiglitazone therapy on gene expression in human adipose tissue in vivo in patients with type 2 diabetes,” Journal of Clinical Endocrinology and Metabolism, vol. 92, no. 2, pp. 720–724, 2007.
- A. M. Sharma and B. Staels, “Review: peroxisome proliferator-activated receptor γ and adipose tissue—understanding obesity-related changes in regulation of lipid and glucose metabolism,” Journal of Clinical Endocrinology and Metabolism, vol. 92, no. 2, pp. 386–395, 2007.
- M. Sánchez-Hidalgo, A. R. Martín, I. Villegas, and C. Alarcón De La Lastra, “Rosiglitazone, an agonist of peroxisome proliferator-activated receptor gamma, reduces chronic colonic inflammation in rats,” Biochemical Pharmacology, vol. 69, no. 12, pp. 1733–1744, 2005.
- A. Ialenti, G. Grassia, P. Di Méglio, P. Maffia, M. Di Rosa, and A. Ianaro, “Mechanism of the anti-inflammatory effect of thiazolidinediones: relationship with the glucocorticoid pathway,” Molecular Pharmacology, vol. 67, no. 5, pp. 1620–1628, 2005.
- H. Xu, G. T. Barnes, Q. Yang et al., “Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance,” Journal of Clinical Investigation, vol. 112, no. 12, pp. 1821–1830, 2003.
- A. L. Hevener, J. M. Olefsky, D. Reichart et al., “Macrophage PPARγ is required for normal skeletal muscle and hepatic insulin sensitivity and full antidiabetic effects of thiazolidinediones,” Journal of Clinical Investigation, vol. 117, no. 6, pp. 1658–1669, 2007.
- M. Loviscach, N. Rehman, L. Carter et al., “Distribution of peroxisome proliferator-activated receptors (PPARs) in human skeletal muscle and adipose tissue: relation to insulin action,” Diabetologia, vol. 43, no. 3, pp. 304–311, 2000.
- A. L. Hevener, W. He, Y. Barak et al., “Muscle-specific Pparg deletion causes insulin resistance,” Nature Medicine, vol. 9, no. 12, pp. 1491–1497, 2003.
- N. K. Verma, J. Singh, and C. S. Dey, “PPAR-γ expression modulates insulin sensitivity in C2C12 skeletal muscle cells,” British Journal of Pharmacology, vol. 143, no. 8, pp. 1006–1013, 2004.
- R. H. Amin, S. T. Mathews, H. S. Camp, L. Ding, and T. Leff, “Selective activation of PPARγ in skeletal muscle induces endogenous production of adiponectin and protects mice from diet-induced insulin resistance,” American Journal of Physiology, vol. 298, no. 1, pp. E28–E37, 2010.
- A. W. Norris, L. Chen, S. J. Fisher et al., “Muscle-specific PPARγ-deficient mice develop increased adiposity and insulin resistance but respond to thiazolidinediones,” Journal of Clinical Investigation, vol. 112, no. 4, pp. 608–618, 2003.
- Y. Masubuchi, S. Kano, and T. Horie, “Mitochondrial permeability transition as a potential determinant of hepatotoxicity of antidiabetic thiazolidinediones,” Toxicology, vol. 222, no. 3, pp. 233–239, 2006.
- J. Sahi, C. B. Black, G. A. Hamilton et al., “Comparative effects of thiazolidinediones on in vitro P450 enzyme induction and inhibition,” Drug Metabolism and Disposition, vol. 31, no. 4, pp. 439–446, 2003.
- A. Guillouzo, “Liver cell models in in vitro toxicology,” Environmental Health Perspectives, vol. 106, no. 2, pp. 511–532, 1998.
- P. Gripon, S. Rumin, S. Urban et al., “Infection of a human hepatoma cell line by hepatitis B virus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 24, pp. 15655–15660, 2002.
- V. Cerec, D. Glaise, D. Garnier et al., “Transdifferentiation of hepatocyte-like cells from the human hepatoma hepaRG cell line through bipotent progenitor,” Hepatology, vol. 45, no. 4, pp. 957–967, 2007.
- A. Guillouzo and C. Guguen-Guillouzo, “Evolving concepts in liver tissue modeling and implications for in vitro toxicology,” Expert Opinion on Drug Metabolism and Toxicology, vol. 4, no. 10, pp. 1279–1294, 2008.
- C. Aninat, A. Piton, D. Glaise et al., “Expression of cytochromes P450, conjugating enzymes and nuclear receptors in human hepatoma HepaRG cells,” Drug Metabolism and Disposition, vol. 34, no. 1, pp. 75–83, 2006.
- J. Sahi, C. B. Black, G. A. Hamilton et al., “Comparative effects of thiazolidinediones on in vitro P450 enzyme induction and inhibition,” Drug Metabolism and Disposition, vol. 31, no. 4, pp. 439–446, 2003.
- V. Ramachandran, V. E. Kostrubsky, B. J. Komoroski et al., “Troglitazone increases cytochrome P-450 3A protein and activity in primary cultures of human hepatocytes,” Drug Metabolism and Disposition, vol. 27, no. 10, pp. 1194–1199, 1999.
- V. E. Kostrubsky, J. F. Sinclair, V. Ramachandran et al., “The role of conjugation in hepatotoxicity of troglitazone in human and porcine hepatocyte cultures,” Drug Metabolism and Disposition, vol. 28, no. 10, pp. 1192–1197, 2000.
- K. P. Kanebratt and T. B. Andersson, “HepaRG cells as an in vitro model for evaluation of cytochrome P450 induction in humans,” Drug Metabolism and Disposition, vol. 36, no. 1, pp. 137–145, 2008.
- J. Yu, L. Qiao, L. Zimmermann et al., “Troglitazone inhibits tumor growth in hepatocellular carcinoma in vitro and in vivo,” Hepatology, vol. 43, no. 1, pp. 134–143, 2006.
- Y. Edling, L. K. Sivertsson, A. Butura, M. Ingelman-Sundberg, and M. Ek, “Increased sensitivity for troglitazone-induced cytotoxicity using a human in vitro co-culture model,” Toxicology in Vitro, vol. 23, no. 7, pp. 1387–1395, 2009.
- M.-Y. Li, H. Deng, J.-M. Zhao, D. Dai, and X.-Y. Tan, “Peroxisome proliferator-activated receptor gamma ligands inhibit cell growth and induce apoptosis in human liver cancer BEL-7402 cell,” World Journal of Gastroenterology, vol. 9, no. 8, pp. 1683–1688, 2003.
- A. Klopotek, F. Hirche, and K. Eder, “PPARγ ligand troglitazone lowers cholesterol synthesis in HepG2 and Caco-2 cells via a reduced concentration of nuclear SREBP-2,” Experimental Biology and Medicine, vol. 231, no. 8, pp. 1365–1372, 2006.
- R. Maniratanachote, K. Minami, M. Katoh, M. Nakajima, and T. Yokoi, “Chaperone proteins involved in troglitazone-induced toxicity in human hepatoma cell lines,” Toxicological Sciences, vol. 83, no. 2, pp. 293–302, 2005.
- H. Koga, M. Harada, M. Ohtsubo et al., “Troglitazone induces p27Kip1-associated cell-cycle arrest through down-regulating Skp2 in human hepatoma cells,” Hepatology, vol. 37, no. 5, pp. 1086–1096, 2003.
- H. Koga, S. Sakisaka, M. Harada et al., “Involvement of p21WAF1/Cip1, p27Kip1, and p18INK4c in troglitazone-induced cell-cycle arrest in human hepatoma cell lines,” Hepatology, vol. 33, no. 5, pp. 1087–1097, 2001.
- C. B. Lambert, C. Spire, N. Claude, and A. Guillouzo, “Dose- and time-dependent effects of phenobarbital on gene expression profiling in human hepatoma HepaRG cells,” Toxicology and Applied Pharmacology, vol. 234, no. 3, pp. 345–360, 2009.
- M.-A. Bae, H. Rhee, and B. J. Song, “Troglitazone but not rosiglitazone induces G1 cell cycle arrest and apoptosis in human and rat hepatoma cell lines,” Toxicology Letters, vol. 139, no. 1, pp. 67–75, 2003.
- C. L. Chaffer, D. M. Thomas, E. W. Thompson, and E. D. Williams, “PPARγ-independent induction of growth arrest and apoptosis in prostate and bladder carcinoma,” BMC Cancer, vol. 6, article 53, 2006.
- F. Yin, D. Bruemmer, F. Blaschke, W. A. Hsueh, R. E. Law, and A. J. Van Herle, “Signaling pathways involved in induction of GADD45 gene expression and apoptosis by troglitazone in human MCF-7 breast carcinoma cells,” Oncogene, vol. 23, no. 26, pp. 4614–4623, 2004.
- T. Satoh, M. Toyoda, H. Hoshino et al., “Activation of peroxisome proliferator-activated receptor-γ stimulates the growth arrest and DNA-damage inducible 153 gene in non-small cell lung carcinoma cells,” Oncogene, vol. 21, no. 14, pp. 2171–2180, 2002.
- L. D. Kier, R. Neft, L. Tang et al., “Applications of microarrays with toxicologically relevant genes (tox genes) for the evaluation of chemical toxicants in Sprague Dawley rats in vivo and human hepatocytes in vitro,” Mutation Research, vol. 549, no. 1-2, pp. 101–113, 2004.
- K. M. O. Goyak, M. C. Johnson, S. C. Strom, and C. J. Omiecinski, “Expression profiling of interindividual variability following xenobiotic exposures in primary human hepatocyte cultures,” Toxicology and Applied Pharmacology, vol. 231, no. 2, pp. 216–224, 2008.
- C.-M. Loi, M. Young, E. Randinitis, A. Vassos, and J. R. Koup, “Clinical pharmacokinetics of troglitazone,” Clinical Pharmacokinetics, vol. 37, no. 2, pp. 91–104, 1999.