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The Scientific World Journal
Volume 2013 (2013), Article ID 436517, 25 pages
http://dx.doi.org/10.1155/2013/436517
Review Article

Lignin: Characterization of a Multifaceted Crop Component

Division of Abiotic Stress Tolerance in Crops, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Karlrobert-Kreiten Straße 13, 53115 Bonn, Germany

Received 5 August 2013; Accepted 24 September 2013

Academic Editors: J. J. Loor, M. Nikolic, B. Uzun, L. Velasco, and B. R. Wilson

Copyright © 2013 Michael Frei. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lignin is a plant component with important implications for various agricultural disciplines. It confers rigidity to cell walls, and is therefore associated with tolerance to abiotic and biotic stresses and the mechanical stability of plants. In animal nutrition, lignin is considered an antinutritive component of forages as it cannot be readily fermented by rumen microbes. In terms of energy yield from biomass, the role of lignin depends on the conversion process. It contains more gross energy than other cell wall components and therefore confers enhanced heat value in thermochemical processes such as direct combustion. Conversely, it negatively affects biological energy conversion processes such as bioethanol or biogas production, as it inhibits microbial fermentation of the cell wall. Lignin from crop residues plays an important role in the soil organic carbon cycling, as it constitutes a recalcitrant carbon pool affecting nutrient mineralization and carbon sequestration. Due to the significance of lignin in several agricultural disciplines, the modification of lignin content and composition by breeding is becoming increasingly important. Both mapping of quantitative trait loci and transgenic approaches have been adopted to modify lignin in crops. However, breeding goals must be defined considering the conflicting role of lignin in different agricultural disciplines.

1. Introduction

Lignin is a complex aromatic polymer, which is deposited in the secondary cell walls of all vascular plants [1, 2]. It is tightly cross-linked with other cell wall components and can thus be considered the “cellular glue” providing strength to plant tissues and fibers and stiffness to the cell walls [3]. Its function in plants also includes the defense against abiotic and biotic stresses, especially pathogens and insects [4], and conferring stability to xylem vessels for efficient water transport [5]. Together with the carbohydrate polymers cellulose and hemicellulose, lignin forms the largest portion of “lignocellulosic” plant materials. Thus, lignin accounts for a substantial portion of the total organic carbon in the biosphere, surpassed only by cellulose [2, 6].

It has been estimated that more than 2 × 1011 tons of lignocellulosic material are produced as agricultural byproducts each year, including straw, roots, husks, bagasse, shells [7]. Cereal production alone produces roughly 2.8 × 109 tons of lignocellulosic crop residue each year [8]. A large portion of these crop residues are traditionally incorporated into soils, but some are used as animal feed; lignocelluloses have recently been identified as an abundant source of feedstock for bioenergy production [7, 9]. With the increasing demand for biomass as feedstock for bioenergy conversion, even the production of specialized biomass crops such as Miscanthus spp. or bioenergy maize is becoming more common, thus adding to the vast pool of lignocellulosic material produced each year [9]. The characteristic feature determining the role of lignin in various applications of lignocelluloses is its resistance to microbial fermentation, whether in living plants, in ruminants’ digestive tract, in soils, or in bioenergy reactors.

This review characterizes the role of lignin from the point of view of analytical chemistry, plant stress physiology, animal nutrition, bioenergy production, soil science, and crop breeding. It focuses on annual crops grown in agro-ecosystems, rather than woody and perennial species and natural ecosystems. By elucidating the role of lignin from the perspective of several disciplines, synergies and conflicts are identified that need to be addressed in crop management, the utilization of crop products and residues, and in plant breeding schemes.

2. Lignin Biosynthesis

Lignin biosynthesis in plants can be divided into three major phases: (i) synthesis of monolignols in the symplastic shikimate and phenylpropanoid pathway, (ii) export of monolignols to the apoplast, and (iii) activation of monolignols by enzyme-mediated formation of monolignol radicals in the apoplast and their polymerization to form complex lignin polymers (Figure 1).

436517.fig.001
Figure 1: Simplified model of lignin biosynthesis in vascular plants. PAL: phenylalanine ammonia lyase, POX: peroxidases, LAC: laccases, and ROS: reactive oxygen species.

The phenylpropanoid pathway is the source of a huge array of secondary metabolites such as flavonoids, anthocyanins, tannins, coumarin, and volatiles [10, 11]. It is based on just a few intermediates of the shikimate pathway, which involves the conversion of the carboxylic acid shikimate to the aromatic amino acid L-phenylalanine. Important steps of the shikimate pathway are localized in the plastids [10]. The deamination of L-phenylalanine to trans-cinnamate is catalyzed by the key enzyme phenylalanine ammonia lyase (PAL), which forms the entry point into the synthesis of all phenylpropanoids [11]. The formation of monolignols further requires hydroxylation of the aromatic ring, methylation of hydroxyl groups, and the stepwise reduction of monolignol side chains from carboxylic acids to alcohols. These steps are mediated by specific enzymes [2] and lead to the formation of three major monolignols, that is, p-Coumaryl alcohol, Coniferyl alcohol, and Sinapyl alcohol.

These monolignols constitute the building blocks for lignin polymers and have to be transported across the plasma membrane to the apoplast. Although the transport of monolignols remains poorly understood, three major models have been proposed [11]: (i) transport of monolignols through vesicles derived from Golgi bodies, (ii) passive diffusion of monolignols through the plasma membrane, and (iii) active transport mediated by plasma-membrane located transporters. Further details of these proposed transport mechanism are discussed in a review by Liu [11]. Overall, monolignol transport remains a poorly understood step in lignin biosynthesis that warrants further research.

Activation of monolignols in the apoplast requires enzymes such as peroxidases (POX), laccases (LAC), or other polyphenol oxidases that transfer electrons from monolignols to electron receptors. These apoplastic enzymes interact with reactive oxygen species (ROS) such as hydrogen peroxide or superoxide, which act as electron receptors or modulators of POX and LAC enzymes through their signaling function [1214]. ROS are formed as byproducts of many metabolic processes in plants, but can also be actively produced through enzymes such as NADPH-oxidases or “class III peroxidases” (also termed as guaiacol POX), and they accumulate excessively when plants encounter abiotic or biotic stress [14, 15]. POX use hydrogen peroxide as an electron receptor to oxidize a variety of phenolic compounds including monolignols [11]. Laccases (LAC) are copper-containing apoplastic enzymes that oxidize phenolic compounds using molecular oxygen as an electron receptor [11]. Generally, plant genomes contain many isoforms of POX and LAC genes with presumably overlapping functions, making it difficult to link particular isoforms of these enzymes to lignin synthesis [2, 16]. After the activation of monolignols by these enzymes, oxidized monolignol radicals couple on to each other to form three dimensionally cross-linked structures. This process is called polymerization and constitutes the final step of lignin biosynthesis.

This section gives only a brief summary of the most important processes involved in lignin biosynthesis. The genetic, transcriptional, and biochemical regulation of lignin biosynthesis in plants is extremely complex and has been discussed extensively (for reviews see [2, 6, 11, 16, 17]).

3. Lignin Content of Crops and Quantitative Measurements

The lignin content of crops depends on multiple factors, such as the growth stage, genotype, morphological fraction, and environmental conditions. Data from studies that surveyed a broad range of herbaceous agricultural crops show that lignin concentration in the vegetative tissue usually ranges from 1 to 15 percent of the dry mass [1826]. The extent of lignification tends to increase with increasing plant age [2730], an effect that appears to be more pronounced in grasses as compared to legumes [22]. In addition, large genotypic differences in lignin content within the same species have been reported. For example, studies on genotypic variation in rice straw lignin reported values ranging from 1 to almost 12 percent [20, 30], although at least some of this variation might be explained by the fact that the analyses were conducted in different laboratories, as detailed below. Another study on the biomass crop Miscanthus revealed a range of 6 to 14 percent in 244 different accessions [19]. Differences also occur between lignification of different morphological fractions. In maize, roots were shown to contain more lignin than aboveground biomass [31], while in rice, differences between lignification of the stem and leaf were not consistent and depended on the genotype [20]. Moreover, abiotic and biotic stress factors tend to affect the lignification of crops [4, 3234], as detailed in the respective sections of this review.

Apart from these factors causing true differences in lignification of crops, substantial variation arises from the analytical method employed in determining lignin concentration. Comparative studies testing different analytical methods reported up to fourfold differences in lignin content of identical samples [18, 3538]. It is thus evident that lignin values obtained using different methods in different laboratories cannot be directly compared.

Methods for determining lignin in crops can be grouped into three categories: gravimetric, spectrometric, and noninvasive methods (Figure 2). Several of these methods require a pretreatment of plant samples to remove non-cell wall components such as proteins, lipids, and nonstructural carbohydrates. The type of pretreatment depends on the sample and may consist of a neutral detergent fiber digestion [39], treatment with hot water-organic solvent, or ethanol-benzene [40]. Gravimetric methods are based on sequential digestion and weighing of cell wall fractions. The “Klason” method represents a classical approach [41] and is based on a two-step digestion of all nonlignin components in sulfuric acid, followed by the recovery and weighing of the residue. The acid detergent lignin (ADL) method [39] is based on pretreatment of the samples with an acid detergent solution (ADS), in which proteins, nonstructural carbohydrates, lipids, pectin, and hemicelluloses are removed, leaving a residue of cellulose and lignin. Cellulose is then removed by sulfuric acid digestion, and the residue is weighed. This method may be inaccurate in samples containing high amounts of cutin or suberin, two compounds which are not removed by ADS [35]. In this case, ADS treated samples are exposed to permanganate oxidation, which decomposes the lignin fraction. Permanganate lignin (PerL) is then obtained by the difference in weight before and after permanganate treatment.

436517.fig.002
Figure 2: Overview of different lignin analysis methods.

Spectrometric methods are based on solubilization of lignin from cell wall preparations and measurement of its specific absorbance at 280 nm. These methods require the removal of potentially interfering substances by pretreatment as described previously, to obtain cell wall preparations. Lignin is then derivatized using acetylbromide [18, 40], HCl triethylene glycol [40], or thioglycolate [35, 42] to render lignin soluble in a suitable solvent. Solubilized lignin can then be quantified by spectrometric measurements at 280 nm using extinction coefficients, but these need to be calibrated for each type of sample [35]. Alternatively, standard curves for quantification can be obtained using standardized lignin preparations [40, 42, 43].

Noninvasive methods take advantage of specific spectra associated with lignin, which can be measured in almost unprocessed samples. However, these methods require calibration using data obtained from gravimetric or spectrometric methods, and the calibration models obtained are specific to a particular type of samples (e.g., rice straw, alfalfa, etc.). Commonly used noninvasive methods include near infrared spectroscopy (NIRS) and nuclear magnetic resonance (NMR) spectroscopy [35, 44]. The advantage of these methods is that, once a calibration model has been established, they are suitable for high throughput analyses in applications such as plant breeding.

The lowest values are usually obtained from the widely used ADL method, presumable because a portion of the lignin is removed during the acid detergent treatment preceding the sulfuric acid digestion, thus leading to an underestimation of true lignin values [35, 36, 45]. However, the data obtained from different methods strongly depends on the type of sample, its content of substances interfering with each of the assays, and its lignin composition. Further details on comparison of analytical methods, including analytical protocols, have been published elsewhere [18, 35, 39, 40, 42].

4. Role of Lignin in Abiotic Stress Tolerance

Lignin in crops interacts with abiotic stresses in two ways: (i) many abiotic stresses influence lignin biosynthesis and therefore affect the lignin content of crops [4, 33], and (ii) lignification of crop tissues affects plant fitness and can confer tolerance to abiotic stresses [46, 47]. The effects of some predominant abiotic stresses on lignin content of crops are summarized in Table 1. This summary considers only studies reporting actual lignin measurements and excludes reports on stress responses of lignin biosynthetic genes and enzymes, without actually measuring lignin. Evidently, most of these studies reported enhanced lignin content in crops grown under abiotic stress.

tab1
Table 1: Effect of abiotic stresses on lignin concentration of different morphological fractions of crops.

In the case of drought, contradicting effects on lignin level have been observed (Table 1). Increases in lignin content observed in maize, clover, and ryegrass were explained with drought-induced activation of lignifying enzymes [48, 53, 56]. The role of lignin in the drought tolerance of maize was also confirmed in experiments in which lignin deficient mutants exhibited drought symptoms even in well-watered conditions [150] and in which leaf lignin levels correlated with drought tolerance in a set of contrasting genotypes [56]. Similarly, transgenic tobacco plants with enhanced lignin levels showed improved tolerance to drought compared to the wild type [151]. However, some studies also reported decreases in the lignin content due to drought. For example, it was found that water deficit decreased the level of lignifying enzymes and consequently the lignin level in maize leaves [57]. Another study reported enhanced drought tolerance in transgenic alfalfa plants, which have lower lignin levels than their wild type [152]. The authors explained these observations with constitutive stress defense gene activation associated with lack of lignin in their alfalfa mutants. In summary, controversial results have been reported from drought experiments, which could be explained by species and genotypic differences, different drought treatments, or different methods employed for lignin measurement.

Unlike drought stress, almost all other experiments reported increases in lignification when crops were subjected to various abiotic stresses (see Table 1), thus highlighting the importance of lignin as a stress response factor. In the case of salinity, lignification of the root was observed in many crop species (Table 1). A transgenic rice line which deposited enhanced levels of lignin in the roots when exposed to salt treatment was more tolerant than its wild type, which did not show such a response [64]. The beneficial effects of lignification were explained with anatomical changes that facilitate water flow and maintain structural integrity of the xylem vessels during salt stress [60, 62], or by lignification of the Casparian strip, which forms a mechanical barrier to ion diffusion in the root endodermis [63, 153, 154]. In fact, lignin was recently shown to be the major component of the Casparian strip Arabidopsis thaliana roots [155]. Lignification has also been proposed to be a factor causing root growth reduction under salt stress due to elevated rigidity of lignified cell walls [66].

Similar to salinity, lignification of the root was observed as a response to mineral toxicities in many crop species (Table 1). When chamomile plants were subjected to short-term (7 days) aluminum (Al) toxicity [156], the effects of lignification varied depending on the concurrent application of biochemical regulators: it increased with the application of salicylic acid but decreased with application of the reducing agent dithiothreitol (DTT). In studies on rice and wheat roots, Al toxicity increased the root hydrogen peroxide levels, which was considered as a redox signal leading to lignification [68, 69]. In a study on flax [71], Al toxicity likewise led to lignification of the root, but this effect was mitigated by application of high doses of boron (B) [71], while excess B solely also increased lignin levels. B toxicity also increased lignification of tomato roots [74], while cadmium and copper toxicity stimulated the lignification of soybean roots [72]. Enhanced deposition of lignin in the root endodermis was also observed under Zn toxicity in the metal hyper-accumulator plant Thlaspi caerulescens,which can tolerate much higher metal levels than other species [157]. Common observations in all of these studies were that mineral toxicities increased hydrogen peroxide levels, which induced lignin biosynthetic enzymes (especially POX) and consequently led to lignification and solidification of the cell walls but also reduced root growth [158].

Mineral deficiencies were also shown to influence the lignin level of crops (Table 1). Nitrogen (N) deficiency led to increases in lignin content of tobacco plants [78], which was explained with a shift from nitrogen containing compounds to carbon-rich phenylpropanoids in the plant tissues. Calcium deficiency induced the activity of enzymes of the phenylpropanoid pathway, which increased the lignin level in soybean roots [79]. Similarly, potassium, phosphorus, and N deficiency increased the lignin content of potato tubers [80], but the authors did not give a physiological explanation for their observation. Manganese (Mn) deficiency appears to be an exception, as it reduced the lignin concentration in wheat roots and shoots [75, 76]. This exception can be explained by the fact that Mn is required for lignin biosynthesis as it activates several enzymes of the shikimate and phenylpropanoid pathway [159, 160].

High tropospheric ozone led to increased lignin levels in the aboveground parts of a number of forage or cereal crops (Table 1). Ozone is a phytotoxic air pollutant which enters the crop leaves during photosynthetic gas exchange and decomposes into reactive oxygen species (ROS) in the apoplast [161, 162]. This leads to an oxidative burst, during which further ROS are produced through the function of enzymes such as NADPH oxidase, followed by a signaling cascade which can lead to cell death and the formation of visible leaf symptoms [163]. Genes and enzymes involved in lignin biosynthesis, such as phenylalanine ammonia lyase (PAL) or POX, are triggered by these processes because they form part of the defense mechanism to contain cell death [164, 165]. Thus, there is broad agreement in the scientific literature that ozone exposure leads to enhanced lignin concentration in crops (Table 1).

Similar mechanisms have been put forward to explain increases in lignin concentration due to elevated UV radiation (Table 1). There is consensus that UV-B or UV-C radiation stimulated the phenylpropanoid pathway leading to enhanced lignification in grasses [88], tomato fruits [89], cucumber seedlings [90], and quinoa seedlings [91], representing a resistance mechanism against oxidative stress. In a study on soybeans [92], high UV-B radiation only increased the level of soluble phenolics but not that of lignin polymers. The authors speculated that the lack of polymerization occurred because samples were taken early in the growing season and emphasized the role of UV-induced phenolics in the protection against insect herbivory as a positive side effect. Similarly, transgenic rice lines with enhanced lignin level showed improved resistance against high UV radiation but also biotic stresses [99].

Apart from its involvement in typical abiotic stresses, lignin has long been assumed to be involved in resistance of crops to lodging, although it remained unclear whether lignin had a positive or negative effect [166]. While lignin could lend mechanical support to the stalks, it may also have the opposite effect of making stalks more brittle and thus susceptible to mechanical damage [166]. This decade-old question is still being discussed in the scientific literature with contradictory results. Some recent work indicated that high lignin levels were associated with lodging resistance in wheat [167, 168] and pea [169], and a quantitative trait locus (QTL) for lodging resistance colocated with a QTL for high lignin content in ryegrass [170]. A study on rice concluded that lignin played an important role in lodging tolerance but suggested that its distribution and density were more important than its concentration [171]. No significant differences in lignin content were seen in wheat cultivars differing in lodging resistance [172], while another study concluded that cellulose rather than lignin conferred resistance to lodging in wheat [173]. In addition, brown-midrib mutations, which were associated with reduced lignin levels, did not affect lodging resistance in maize, sorghum, and pearl millet [174]. A study on a maize mutant with drastically reduced mechanical strength suggested that cellulose rather than lignin deposition in the stalk was associated with susceptibility to lodging [175]. In summary, the question of whether lignin confers tolerance to lodging remains unanswered.

5. Role of Lignin in Biotic Stress Tolerance

The cell wall constitutes the first line of defense of plants against pathogens such as bacteria and fungi, nematodes, or herbivorous insects [176, 177]. Lignin solidifies the cell wall, providing a nondegradable barrier for pathogens, and is therefore thought to enhance its protective effect against such biotic stresses [4, 178]. Enhanced lignin biosynthesis due to biotic stress has been ascribed to stimulation of the phenylpropanoid pathway and the induction of apoplastic lignin polymerization [95, 179]. These defense reactions are mediated by ROS-induced signaling cascades. A common response of plants to biotic stresses has been termed “oxidative burst,” and involves the active production of apoplastic ROS [14, 180, 181]. This can be mediated by NAD(P)H-oxidases, that is, plasma-membrane bound enzymes transferring electrons from cytosolic NAD(P)H to extracellular oxygen to produce superoxide. This particularly aggressive ROS is dismutated to hydrogen peroxide by the enzyme superoxide dismutase (SOD) [180]. Alternatively, an oxidative burst can be mediated by apoplastic enzymes such as class III POX [13] or polyamine oxidases [181]. Apoplastic ROS serve as signaling molecules to induce defense reactions and serve as electron receptors for lignification, which facilitates the containment of pathogens and wound-healing.

These processes have been observed in numerous studies investigating a broad range of biotic stresses affecting model plants, woody plant species, and agricultural crops. Experiments supporting the protective effects of lignification against biotic stresses in agricultural crops are summarized in Table 2. The broad spectrum of different crops and pathogens/insects highlights the general applicability of the principle that lignin constitutes a biotic stress tolerance factor. The majority of the experiments reporting a protective role of lignin dealt with fungal pathogens, suggesting that lignification is particularly effective against this category of pathogens. Fewer studies dealt with bacteria, nematodes, and insects. The experiments summarized in Table 2 can be grouped into three categories. (i)The first category includes studies reporting indirect or correlative evidence for the involvement of lignin in tolerance to abiotic stresses. Evidence was based on contrasting lignin levels or differential stress responses of lignin biosynthetic pathways in tolerant and intolerant genotypes. While such studies are informative and quite abundant, their weakness is that unrelated crop genotypes presumably differed in many traits apart from lignification, which may also have influenced their tolerance. This category represents the majority of the studies summarized in Table 2. (ii)The second one includes studies in which genes affecting lignin biosynthesis were specifically manipulated to obtain near-isogenic lines of crops differing in lignin content. This approach was taken in a number of experiments using diverse crop species such as wheat [96], rice [99], tobacco [103, 104, 121, 130], cotton [108], potato [111], and carrot [113]. Either gain of function (to increase lignin) or loss of function (to decrease lignin) mutants were used in these experiments to obtain direct evidence of the involvement of lignin in tolerance against biotic stresses. (iii)The third category includes studies applying pretreatment with elicitors to induce systemically acquired resistance in crops, which involved enhanced lignin content in pretreated plants. These studies used either pretreatment with biological elicitors such as Pseudomonas ssp. [120], fungal extracts [115, 117], chemical compounds such as salicylic acid, dichloroisonicotinic acid, or bion (benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester [109, 119], or mechanical injury [93]. These approaches were shown to enhance lignin levels and, consequently, resistance to biotic stresses in orange fruits [93], cotton roots [109], tomato [115], peanut [117], cucumber [119], and rice [120].

tab2
Table 2: Summary of studies suggesting lignification as an effective defense mechanism against biotic stresses in agricultural crops.

Despite the abundant evidence for the protective role of lignin against biotic stresses, there are also reports in which the lignin level did not positively affect crops resistance. Lignin concentration was not a factor differentiating Fraxinus cultivars resistant or susceptible to emerald ash borer [182]. Also, lignin levels did not explain the resistance of five different switchgrass (Panicum virgatum) populations to aphids and aphid-transmitted virus diseases [183]. In sorghum, low lignin “brown-midrib” genotypes even exhibited reduced colonization by Fusarium ssp. and Alternaria alternata [184]. The authors suggested that impairment of lignin synthesis could shift intermediates of the phenylpropanoid pathway to different branches, which also have protective effects against pathogens. Overall, these exceptions are relatively rare and do not necessarily contradict the majority of studies supporting the important role of lignin in plant defense against biotic stresses.

Because the cell wall constitutes such an important physical barrier, pathogens have evolved a broad array of enzymes to digest lignocelluloses [185]. A class of fungi called white rot fungi possesses the particular ability to decompose lignin via extracellular enzymes such as lignin peroxidase, manganese peroxidase, and laccase [186188]. These fungi are increasingly being used in agricultural or industrial applications that require the removal of lignin from plant material, such as ruminant nutrition or bioethanol production [189191]. The digestive systems of herbivorous insects do not have the capacity of decomposing lignin, but some insects host lignin degrading fungi in their stomachs to facilitate the digestion of lignified plant material [192].

6. Role of Lignin in Animal Nutrition

From a point of view of animal nutritionists, lignin represents an undesired or “antinutritive” component. Being part of the cell wall, lignin forms a limiting factor especially in the diets of ruminant herbivores, which unlike monogastric animals are able to digest cell wall material efficiently. With the aid of their anaerobic rumen microbial population, ruminant herbivores ferment polysaccharide polymers (cellulose and hemicelluloses) into short chain fatty acids, which serve as a source of energy for the animal, while the microbes themselves form a source of protein [193, 194]. The extremely diverse rumen microbial population produces many glycosyl hydrolases, that is, enzymes that hydrolyze the glycosidic bonds between carbohydrates, or between carbohydrate and noncarbohydrate molecules [193]. In contrast, lignin is not readily fermented by the rumen bacteria but is only partly degraded by rumen anaerobic fungi [193]. As a consequence, it limits the feed value of plant materials through two mechanisms. (i) Inaccessible energy content: although lignin has about 30 percent higher gross energy content than cellulose [195], this energy is barely accessible for ruminants. Therefore, the lignin content is negatively correlated with digestible energy in ruminant diets. (ii) Reduced feed intake: due to the association with polysaccharide constituents, lignin forms a physical barrier and thus hinders the access of rumen microbes to fermentable cell wall components. Consequently the passage rate of feeds through the rumen is slowed down, thus reducing the feed intake capacity [196, 197].

The negative correlation between lignin content and digestibility of forage materials in ruminant diets has been documented in numerous experiments. A common approach to determine the feed value of plant materials for ruminates involves the incubation of samples in rumen liquor in vitro to measure digestibility. This can be accompanied by time-course measurements of the amount of gas produced during fermentation, which is positively correlated with digestibility [198]. Using such techniques, lignin was identified as a dominant factor limiting the feed value in perennial grasses [199], maize stems [200, 201], and tropical forages [202]. In an experiment with alfalfa, it was shown that lignin content had a more negative effect in long-term than in short-term in vitro incubations, indicating that it affected the potential extent of digestion rather than the rate of digestion [203]. Similar relationships were also found in artificially modeled diets: in incubation experiments with maize cell walls that were artificially lignified using monolignol treatments, the lignification caused up to a 12-fold increase in the lag time of cellulose fermentation [204]. Animal feeding experiments in principle confirmed these in vitro experiments. For example, it was demonstrated that lignin was the main chemical parameter explaining the in vivo organic matter digestibility of 64 different grass silages fed to cattle [205]. Another study reported a negative correlation between lignin concentration and in vitro digestibility in 36 different forages including legumes, C3 grasses, and C4 grasses and confirmed these results in feeding trials with lambs [206].

Models have been established to predict cell wall digestibility from the degree of lignification. Traxler et al. [207] identified highly positive correlations between lignin content and the indigestible cell wall fraction of 145 different forages and used these data to develop models for predicting digestibility based on lignin concentrations. Similarly, Kramer et al. [22] concluded that the indigestible fraction of plant materials can be estimated from the lignin concentration but also recommended that the same models cannot be applied across different species.

In contrast to the well-documented negative impacts of lignification on forage digestibility, a few studies have also reported positive effects of lignin in ruminant diets relating to greenhouse gas emissions. Ruminant production is one of the most important sources of anthropogenic methane, which constitutes the second most important greenhouse gas next to carbon dioxide [208]. In the rumen, methane is produced during the anaerobic fermentation of organic materials, and released into the atmosphere [209]. When purified lignin was added to lamb diets at different rates, it reduced the feed intake but did not affect growth performance. However, it decreased the methane release in in vitro incubations of lamb feed formulations [210]. Similarly, high lignin diets exhibited relatively low methane release during in vitro incubations in rumen liquor obtained from a cow compared to high sugar diets [211]. In addition, when different types of roughage were incubated in buffalo inoculum, a negative correlation between lignin content and methane release was noted [212]. Together, these studies suggest a positive role of lignin in mitigating methane emissions from ruminant production.

Despite these rare examples of positive effects of lignin, animal nutritionists usually seek to minimize the lignin content of ruminant diets. Two strategies are discussed in the scientific literature: (i) pretreatment of forages to remove lignin prior to feeding them to animals and (ii) breeding of novel low lignin genotypes of forage crops.

Pretreatments to limit the negative effect of lignin on forage digestibility include biological, physical, and chemical processes [213]. The most widely used biological pretreatment of forages involves the use of white rot or brown rot fungi. These lignin degrading fungi produce several types of extracellular oxidative enzymes such as laccases and lignolytic peroxidases [214]. This ability has been used to improve the feed value of low quality forages such as wheat straw [215217], rice straw [218], oil palm fronts [219], Bermuda grass [220], and bamboo [221], just to name a few. In these studies, plant materials were incubated either with fungal inoculum or with isolated enzymes [217] for several days up to fifteen weeks. These treatments were shown to effectively decrease lignification of forages and thus improve their digestibility in ruminant diets. Physical pretreatments such as grinding and steaming usually aim at improving the access of rumen microbes to fermentable cell wall components [213] but may not directly affect lignin content [222]. Chemical treatments involve the extraction of lignin from forages using solvents such as NaOH/ethanol [223] or oxidants such as peracetic acid or hydrogen peroxide [213].

Efforts to breed for low lignin content in forage crops comprised both conventional breeding and biotechnological approaches [224]. Selection for high in vitro digestibility in four perennial forages was associated with simultaneous selection for low lignin content [47]. Naturally occurring or induced brown midrib mutations are known to reduce the lignin content in a number of grass species [174] and were also associated with improved digestibility in maize [200, 225, 226], sorghum, and sudangrass [227, 228].

A number of studies also tested crop or model species that were genetically modified to contain lower lignin content. The downregulation of different monolignol biosynthetic genes to engineer transgenic alfalfa plants containing less lignin led to improved digestibility in independent experiments [229, 230]. Similar results were reported from transgenic maize in which a gene encoding a lignin biosynthetic gene was suppressed [231]. In contrast, changes in lignin composition (but not lignin quantity) due to manipulation of a gene involved in monolignol synthesis (ferulate-5-hydroxylase) did not affect the in vitro digestibility of Arabidopsis thaliana plants [232]. Together, these studies clearly demonstrate that genetic approaches are effective in reducing the lignin content and improving the digestibility of forages.

7. Role of Lignin in the Bioenergy Sector

Lignocellulosic crops or crop residues constitute one of the most abundant resources for the expansion of the renewable energy sector [9, 233, 234]. The role of lignin for energy production from biomass is ambivalent. Whether it constitutes a desired or undesired component essentially depends on the energy conversion process. In thermochemical conversion processes, especially in direct combustion, high lignin content improves the energetic value of biomass. It contains less oxygen than cellulose and hemicellulose and has a heating value of 22–24 kJ g−1, which is 30 to 50 percent more than that of other cell wall components such as cellulose and hemicellulose [195, 235, 236]. In contrast, lignin is inhibitory to biological conversion processes such as microbial fermentation for bioethanol or biogas generation [235, 237].

In direct combustion of lignocellulosic material, the heating value of biomass is strictly positively correlated with its lignin content [238, 239]. Direct combustion has several advantages in small-scale applications: it is straightforward, does not require any processing or investments, and is also cheap and flexible [238]. These potential advantages favor the use of crop byproducts as a source of energy in homes and small industries in developing countries [238, 240]. However, direct combustion has the disadvantage of substantial air pollution and low energy density of unprocessed biomass, making large scale storage and transport unprofitable [238]. Therefore, lignocellulosic biomass is usually processed into more practicable forms such as liquid fuels or combustible gases.

Cell wall material, including cellulose, hemicelluloses, and lignin, can be converted to liquid fuels by pyrolysis. This thermochemical process involves high-temperature heating in the absence of air or oxygen to produce a pyrolysis oil, a complex mixture of components that is generally a low-quality fuel in itself, but can also be upgraded by further processing [241]. Pyrolysis oils are very diverse in their composition, as illustrated by a study which identified 167 different compounds in the pyrolysis oil obtained from rice husks [242]. Pyrolysis has the added benefit of producing char as a byproduct, a stable carbon sink which can be used as a natural soil amendment (so-called “bio-char”), and sequester carbon dioxide [243]. Due to its chemical structure and highly cross-linked nature, lignin has a higher thermal resistance than cellulose and therefore requires higher temperatures for pyrolysis [244]. Boateng et al. [245] compared the performance of 20 alfalfa samples differing in lignin content in two energy conversion processes: high energy pyrolysis and biochemical fermentation by rumen microbes. While biochemical conversion was negatively correlated with lignin content, no negative impact of lignin on pyrolysis yield was noted. Similarly, Fahmi et al. [246] suggested that lignin did not negatively affect pyrolysis yield, but it may lead to the presence of unstable high molecular weight compounds in the pyrolysis oil, which lower the oil quality. Hodgson et al. [247] found little variation in lignin-derived pyrolysis products in a set of Miscanthus genotypes differing in their lignin content and concluded that a substantial proportion of the lignin remained unpyrolyzed at the temperature used in their study (500°C). Another study demonstrated that corn stover pretreated with white rot fungi to break down lignin polymers prior to pyrolysis improved the efficiency of thermochemical conversion of lignin [248]. These examples illustrate that the efficiency and quality of lignin pyrolysis products are variable due to the complex structure and heterogenic composition of lignin. Pyrolysis of lignocelluloses therefore requires optimization of the processing conditions based on the particular species and applications in mind.

Gasification constitutes an alternative thermochemical conversion process. It involves the conversion of solid biomass to syngas (CO + H2) at high temperatures (usually >700°C) with controlled amounts of oxygen, steam, or a mix of gases [249]. After some purification steps, syngas is used in gas turbines or catalytically converted to liquid fuels such as ethanol, although this process remains technically challenging [250]. The gasification of lignin produces four times more hydrogen than cellulose and almost four times more than hemicelluloses [251]. Therefore, high lignin content is considered a favorable trait in biomass used for gasification, and pretreatments often aim at increasing the lignin content. Composting was shown to effectively increase the lignin content of different types of biomass (Leucaena leucocephala, Chamaecytisus palmensis), which in turn led to increases in hydrogen yield in gasification [252, 253]. In summary, it can be concluded that direct combustion and gasification constitute the most effective thermochemical conversions processes for high lignin biomass [236], while pyrolysis may lead to variable results.

In biological energy conversion processes, lignin poses problems very similar to those experienced by animal nutritionists as it constitutes an indigestible component and a mechanical barrier to microbial fermentation of cell wall polysaccharides. The production of bioethanol involves saccharification of cell wall carbohydrates, that is, the enzymatic hydrolysis of cell wall polysaccharides into simple sugars by inoculation with cellulases, followed by fermentation into ethanol by yeast species such as Saccharomyces cerevisiae [254]. Due to its inhibitory role, lignin is sometimes removed from biomass prior to saccharification using biological or chemical pretreatments [254].

The inhibitory role of lignin in bioethanol production has been demonstrated in many studies. For example, lignin content was negatively correlated with sugar release during saccharification of wheat straw [255]. A similar relationship was reported for the bioenergy species Miscanthus, where lignin content was the major determinant of enzymatic biomass degradation [256]. Some authors undertook simultaneous measurements of feed value of forages in ruminant diets and potential ethanol yield and found positive correlations as expected. For example, corn stover in vitro digestibility was positively and lignin content was negatively correlated with ethanol yield in corn stover [257]. Anderson et al. demonstrated a positive correlation between ethanol yield and digestibility for ruminants in 50 Bermuda grass accessions, but lignin explained only a small proportion of the variation [258]. Similar results were obtained in experiments with transgenic crops in which lignin level had been manipulated. Transgenic alfalfa plants engineered to contain less lignin than their wild type showed improved saccharification efficiency [259]. Similarly, the reduction of the lignin content in switchgrass by downregulation of a lignin biosynthesis gene improved the ethanol yield by up to 38% and reduced the need for pretreatment of feedstock, as well as the doses of cellulases required for saccharification [260]. While lignin clearly limits the ethanol production from biomass, the unfermented residues of saccharification and fermentation, which contain high levels of lignin, can be reused for thermochemical energy conversion, especially direct combustion, to produce heat and electricity [261]. Alternatively, lignin may be removed from the biomass prior to the saccharification and recovered for diverse applications using chemical precipitation methods [262].

An alternative biological method to process biomass into energy is the generations of biogas using anaerobic microbial digestion. The term biogas refers to a mix of combustible gases such as methane and hydrogen, which are formed by mixed microbial cultures digesting biomass in anaerobic reactors [263, 264]. There is broad agreement in the scientific literature that lignin is a major factor limiting the biogas yield in anaerobic digestion [264], as illustrated by both experimental and modeling studies. For example, the methane yield in a variety of crops (maize, sorghum, and Miscanthus) depended mostly on the polysaccharide to lignin ratio of the feedstock [265]. Another study tested the biogas production of 57 different plant samples and concluded that a lignin (ADL) content of 10% was a critical threshold for high biodegradability in anaerobic digestion [237]. Also, a significant negative correlation between lignin content and methane production was observed in 285 different maize genotypes [266]. Several predictive models to estimate the biogas yield from lignocellulosic material include lignin as the major negative factor [267269]. Because lignin is such an important factor limiting biogas yield, pretreatments of feedstock often aim at removing lignin from biomass [270, 271]. Such pretreatments include the chemical extraction of lignin using a variety of solvents [270], biological treatments with lignin degrading fungi or enzymes [270, 272, 273], oxidation of lignin using oxidants such as hydrogen peroxide [274], or heat treatments in combination with extraction or oxidation [275].

Regarding the role of lignin, the challenges faced in animal nutrition and in the bioenergy generation via biological conversion are very similar and require better understanding of lignin synthesis and its breakdown. A common strategy that has been proposed in both fields of research is the engineering of modified lignin polymers that are less inhibitory to enzymatic breakdown, while maintaining the functional roles of lignin in adaptation of crops to abiotic and biotic environmental conditions [276, 277].

8. Role of Lignin in Soils

In agricultural soils, lignin has important implications for the soil organic matter (SOM) cycling, thus affecting soil structure, mineralization of nutrients, and carbon (C) sequestration. Traditionally it was assumed that lignin forms a relatively stable component of SOM due to its recalcitrant chemical structure and its resistance to microbial degradation. In this model, the predominant fate of lignin derived from crop residues is the conversion into relatively stable humic substances via aromatic residues of lignin polymers [278, 279]. However, this concept is under debate since more recent research suggests that selective preservation of lignin occurs only in the earlier stages of litter decomposition, whereas found that lignin derived compounds do not accumulate in the refractory C pool of soils [280284]. The low level of associations of lignin with soil minerals has been put forward as a possible explanation for its low accumulation in stable carbon pools [284, 285]. Breakdown of lignin in soil is a predominantly aerobic process mediated by microorganisms such as basidiomycete fungi (brown rot and white rot fungi) and a few species of bacteria such as Streptomyces spp. [278, 286, 287]. These microorganisms produce extracellular enzymes such as phenol oxidases and POX [288] that are also employed in pretreatments of ruminant feed or bioenergy feedstock as described in the previous sections. Besides this predominant biotic decomposition of organic matter, abiotic decomposition also occurs due to photo-degradation. Lignin was shown to be more susceptible to photo-degradation than other SOM components because it acts as an effective light absorbing compound over a wide range of wavelengths [289]. Soil scientists have developed indicators to characterize the lignin degradation state in soils, such as the acid (Ac)/aldehyde Ac/Ald ratio, which is determined after the oxidation of samples with CuO to release single ring phenolics. Ac/Ald indicates the ratio of oxidized (carboxylic acid) to more reduced (aldehyde) forms of lignin derived phenolics (such as vanillic acid to vanillin) and increases upon biodegradation of lignin [286, 290].

Despite the controversy regarding the long-term fate of lignin in soils, there is broad agreement that lignin is a factor that slows down the mineralization of nutrients from crop residues on the time scale of a cropping season. The lignin concentration and the lignin/N ratio are widely used as indicators for the degradability of litter [291, 292]. For example, lignin negatively affected the short-term N release in rice soils from different types of green manure differing in lignin content, including legumes, azolla, and rice straw [293, 294]. Similarly, N release rate was limited by the lignin/N ratio in a study testing mineralization of nutrients from 12 different plant materials in tropical hillside soils [295]. Remarkably, the authors of this study suggested that nutrient mineralization rate from green manures can be estimated by feed value analyses such as in vitro dry matter digestibility, which was confirmed in an investigation of a range of subarctic plant species [296]. The influence of lignin on organic matter decomposition is time dependent and becomes more dominant as decay proceeds, as illustrated in several studies. Taylor et al. [292] suggested that lignin/N ratio was a poor indicator for litter decomposition during the first two months of organic matter incubation in soil but it became more dominant thereafter. This result is congruent with another study [297], in which N released from tropical manure incorporated into soil was not correlated with lignin levels during the first eight weeks of incubation. Similarly, the dissolved organic carbon released from litter decomposition was not affected by lignin during the first five months of litter decomposition, but thereafter it was affected by lignin quantity and quality [298]. In a study on maize roots from 16 different genotypes differing in lignin content and composition, lignin showed no correlation with cumulative C mineralization during the first two weeks of incubation in soil, but showed significant negative correlation from two weeks up to 26 months of incubation [299]. Differences in time scales between studies may be explained with different types of organic matter, different soils, and incubation conditions used. Lastly, lignified biomass with slow mineralization of nutrients could also be interpreted positively as a sustainable fertilizer. Congruent with this concept, artificial ammonoxidized lignin was suggested as a soil amendment combining slow but sustainable nitrogen release with a carbon sequestration function [300].

SOM contains two-thirds of the terrestrial C storage in the world [290, 301] and therefore forms a crucial C sink with respect to global change. Lignin is considered to play an important role in C sequestration in soils [302] and is typically considered a recalcitrant carbon pool in models estimating the CO2 release from SOM decomposition [291]. In addition, a number of experimental studies proposed beneficial roles of lignin in C stabilization in soils. Dijkstra et al. [303] monitored SOM decomposition as affected by N inputs, plant species, and elevated CO2 and concluded that the lignin content of plant litter was a crucial factor determining C stabilization in a grassland ecosystem. Similarly, high lignin content of soil amendments such as compost was considered as a factor leading to stabilization of soil organic C in nonlabile pools in flooded rice ecosystems [304]. In contrast, an increasing number of studies found that lignin derived compounds did not selectively accumulate in the refractory C pool of deeper soil horizons [280283], suggesting that the role of lignin in long-term C sequestration remains to be fully elucidated. Moreover, feedback reactions of global change on carbon cycling in soils are expected, because processes such as lignin decomposition are influenced by environmental factors such as rising temperatures [305]. In a soil warming experiment, the presence of lignin degrading fungi was increased and the degradation of lignin was accelerated at higher temperatures, which would imply faster rates of lignin decomposition in future climates [306]. In contrast, when soil samples were taken from 18 different grassland sites across temperature transects, cool climate favored higher Ac/Ald ratio, indicating a higher degree of microbial lignin decomposition [285]. Such apparent contradictions might be explained with different methodological approaches, that is, a single site experiment in which one factor (soil temperature) was varied versus a multisite study. In conclusion, important questions regarding the potential of lignin to contribute to carbon sequestration in a changing environment are under debate and remain to be answered.

9. Lignin as a Target for Crop Breeding

Lignin content or composition is not a classical target trait in crop breeding, which tends to be more focused on crop yields, stress resistance, or consumer quality of edible crop parts. More recently, molecular breeding techniques in combination with high throughput phenotyping have allowed for more targeted inclusion of specific quality traits such as lignin content in crop breeding schemes. As detailed in the previous sections, lignin content of crops potentially constitutes an important breeding target from the perspectives of several agricultural disciplines. In the past, crop breeding projects were motivated mostly by the role of lignin in animal nutrition and biofuel production and thus aimed at decreasing the lignin content of crops. In principle, three approaches have been adopted in breeding crops with modified lignin content: (i) use of naturally occurring or induced brown midrib mutations, which affect the lignin content; (ii) mapping of quantitative trait loci (QTL) influencing the lignin content, which can be applied in marker assisted selection; (iii) genetic modification of lignin biosynthetic genes to generate transgenic crops with altered lignin content or composition.

Brown midrib mutations were discovered in the 1920s in maize plants, which showed reddish-brown pigmentation of the leaf midrib [307]. Subsequently, four genes causing this phenotype in maize were identified that originated from natural mutations and were named bm1, bm2, bm3, and bm4. It was later discovered that these mutations caused reduced lignin levels [307]. Meanwhile, brown midrib mutants have been isolated in other C4 grasses such as sorghum and pearl millet, arising either through spontaneous or chemically induced mutations. Some genes underlying brown midrib loci have been identified in maize and sorghum. They encode orthologues of lignin biosynthetic genes such as caffeic-O-methyltransferases and O-methyltransferase [174, 308]. Moreover, candidate genes for a further brown midrib locus in maize (bm6) were proposed by genetic mapping [309]. Brown midrib mutants form an excellent model for investigating implications of lignin for crops and have been characterized regarding their resistance to biotic stresses [184], feed quality [227, 310, 311], biofuel potential [312314], and degradability in soil [315].

QTL mapping and marker assisted selection take advantage of the naturally occurring genetic variation in lignin content occurring within crop species [20, 266, 316, 317]. QTL associated with lignin content were reported for a number of crop species (Table 3). The majority of these studies dealt with maize, but a few studies also investigated other species such as barley, sorghum, and rice (Table 3). The primary research objective of most experiments was to improve the feed quality for ruminants by lowering the lignin content, and lignin content was only one among several feed quality parameters for which QTL were reported. However, a few studies, especially on sorghum, were primarily designed to increase the bioenergy potential of the crop (Table 3). In most populations, a fairly large number of QTL were detected with low or intermediate effects, individually explaining up to twenty percent of the phenotypic variation in lignin content, as indicated by partial values (Table 3). In maize, only one major QTL explaining 43 percent of Klason lignin (KL) content was reported in a recent study [142]. Remarkably, this latter study found no colocalization between QTL for KL and ADL, indicating that these two types of lignin represent different fractions of the cell wall. Summarizing their work with six different mapping populations of maize, Barrière et al. [142] assembled an inventory of 50 QTL for ADL, which corresponded to 23 positions in the maize genome. Some QTL for lignin content in crops colocalized with in vitro dry matter digestibility [316], genes involved in lignin biosynthesis [135], or regulatory elements involved in cell wall synthesis [142]. Surprisingly, in some maize populations (e.g., [138]) no colocalization of QTL for lignin and digestibility was observed, indicating that other factors were limiting the digestibility for ruminates. Apart from maize, a major QTL was reported in rapeseed explaining 39 percent of the variation in ADL [149]. Subsequent fine mapping and sequencing of a candidate gene revealed that a polymorphism in the lignin biosynthetic gene cinnamoyl Co-A reductase 1 was probably responsible for differences in seed lignin content. Besides classical QTL mapping for simple quantitative traits such as lignin content, eQTL (expression quantitative trait locus) mapping has more recently been developed to identify genomic regions associated with gene expression patterns associated with a particular phenotype. This approach was used by Shi et al. [318], who selected 439 candidate genes associated with altered cell wall composition in brown midrib maize mutants, and determined eQTL regulating their expression.

tab3
Table 3: Summary of studies reporting QTL for lignin content of different crop species.

While QTL experiments, such as those summarized in Table 3, typically use biparental populations, genome-wide association mapping is emerging as a powerful tool to map genes for quantitative traits in populations of unrelated individuals. This approach has the advantage of sampling more genetic diversity and avoids time consuming generation of crosses necessary for QTL mapping [319]. A genome-wide association study identified loci associated with leaf metabolites in 289 diverse maize lines genotyped with 56 110 SNP markers and reported a locus significantly associated with the level of the lignin precursor p-coumaric acid, which was also correlated with lignin content [320].

Alternatively, lignin content of crops was modified by transgenic approaches [321]. Genetic engineering strategies included the manipulation of lignin biosynthesis at the regulatory level, controlling monolignol biosynthetic enzymes, and modification of lignin polymer structure [277, 321]. Gene knock-down using RNA interference (RNAi) or antisense techniques was successfully employed in a number of crop species targeting different lignin biosynthetic genes, especially those involved in monolignol synthesis. Silencing of genes encoding cinnamyl alcohol dehydrogenase in maize [322] and alfalfa [229] induced changes in lignin composition rather than notable changes in lignin content but significantly affected the digestibility of transgenic lines. O-methyltransferase genes were down-regulated in transgenic maize [231, 323], alfalfa [324], sugarcane [325], and switchgrass [260]. The suppression of gene expression in these species was associated with a decrease in lignin content by up to 30 percent and altered lignin composition, as well as improved digestibility and bioenergy potential. Similarly, the downregulation of three cytochrome P450 genes involved in monolignol synthesis reduced the lignin content by up to 40 percent and altered the lignin composition in transgenic alfalfa [326]. While all of these studies targeted specific monolignol biosynthetic genes, Fornal et al. [327] identified a transcription factor that suppressed the expression of several monolignol biosynthetic genes and proposed it as a good candidate for manipulating the lignin biosynthesis. In addition to these studies with agricultural crops, a large number of mutants of model plants with altered lignin content or composition have been reviewed previously [328].

Concerns have been raised that the breeding of low lignin crops may unintentionally compromise plant fitness by increasing their susceptibility to abiotic or biotic stresses, or by decreasing the plant rigidity and biomass yield [47, 329, 330]. A possible solution to this dilemma could be the breeding of plants with altered lignin composition, which would be less inhibitory towards microbial fermentation in ruminant diets of bioenergy production. More specifically, the substitution of traditional monolignols by alternative monomers with reduced hydrophobicity or cross-linking to structural carbohydrates has been proposed [276, 331, 332].

10. Summary and Conclusions

The previous sections elucidated the processes and factors affecting lignin deposition in crops, as well as the sometimes conflicting role of lignin in various agricultural disciplines. To summarize these considerations, a conceptual model of factors determining lignification of crops and implications for the utilization of lignocellulosic biomass is suggested (Figure 3). Lignification depends on many abiotic and biotic environmental factors. In particular, the presence of environmental stresses tends to increase lignification in most cases, as detailed in the respective sections of this review. Moreover, the lignin content of crops depends on genetic factors such as species, genotype, and specific genes or loci, which are exploited in the breeding of crops with altered lignin content or composition. There are also numerous genotype-by-environment interactions influencing lignification, as evidenced, for example, by the fact that many of the QTL studies summarized in Table 3 detected completely different QTL for lignin content when the same populations were grown in different environments. A better understanding of such genotype-by-environment interactions may be one of the major challenges in developing crops with customized lignin content or composition.

436517.fig.003
Figure 3: Conceptual model of factors influencing the lignification of crops and its implications for different agricultural applications.

Whether high or low lignin is desired depends largely on the use of lignocellulosic material. Applications that favor high lignin content include the breeding of crops resistant to biotic and abiotic stresses, the use of biomass in thermochemical energy conversion processes, and carbon sequestration in recalcitrant biomass (Figure 3). On the other hand, applications that favor low lignin content include the feeding of biomass to ruminant herbivores, biological energy conversion processes such as ethanol or biogas production, and use of crop residues as a nutrient stock for subsequent crops. Exploiting synergies and harmonizing the apparently conflicting roles of lignin remain a major challenge for research, which requires interdisciplinary approaches. A growing number of studies take account of these diverse perspectives by bridging different disciplines. For example, feed digestibility tests using rumen liquor have been used to estimate the degradability of biomass in other media such as soil [295, 296] or bioenergy reactors [257]. Wang et al. [99] reported that enhanced lignin content due to the overexpression of a transcriptional regulator conferred tolerance to both abiotic stress (UV-B) and biotic stresses such as rice blast and white backed planthopper. Breeders have recognized that the breeding for low lignin content to enhance the biological degradability of lignocelluloses may compromise plant fitness and stress resistance [46], although this problem may be overcome by manipulating lignin composition instead of lignin content [276]. However, harmonizing all of the conflicting roles of lignin in the diverse disciplines may not always be possible, thus necessitating priority setting regarding the use of lignocellulosic biomass.

Conflict of Interests

The author declares that there is no conflict of interests regarding the publication of this paper.

Acknowledgment

The author wishes to apologize to those authors whose work was overlooked or not considered due to lack of access or capacity constraints. This work was funded by Deutsche Forschungsgemeinschaft (DFG, FR2951/1-1).

References

  1. P. Sarkar, E. Bosneaga, and M. Auer, “Plant cell walls throughout evolution: towards a molecular understanding of their design principles,” Journal of Experimental Botany, vol. 60, no. 13, pp. 3615–3635, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. N. D. Bonawitz and C. Chapple, “The genetics of lignin biosynthesis: connecting genotype to phenotype,” in Annual Review of Genetics, A. Campbell, M. Lichten, and G. Schupbach, Eds., vol. 44, pp. 337–363, 2010.
  3. E. M. Rubin, “Genomics of cellulosic biofuels,” Nature, vol. 454, no. 7206, pp. 841–845, 2008. View at Publisher · View at Google Scholar · View at Scopus
  4. J. C. Magalhaes Silva Moura, C. A. Valencise Bonine, J. de Oliveira Fernandes Viana, M. C. Dornelas, and P. Mazzafera, “Abiotic and biotic stresses and changes in the lignin content and composition in plants,” Journal of Integrative Plant Biology, vol. 52, no. 4, pp. 360–376, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. S. L. Voelker, B. Lachenbruch, F. C. Meinzer, P. Kitin, and S. H. Strauss, “Transgenic poplars with reduced lignin show impaired xylem conductivity, growth efficiency and survival,” Plant, Cell and Environment, vol. 34, no. 4, pp. 655–668, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. Q. Zhao and R. A. Dixon, “Transcriptional networks for lignin biosynthesis: more complex than we thought?” Trends in Plant Science, vol. 16, no. 4, pp. 227–233, 2011. View at Publisher · View at Google Scholar · View at Scopus
  7. C. O. Tuck, E. Perez, I. T. Horvath, R. A. Sheldon, and M. Poliakoff, “Valorization of biomass: deriving more value from waste,” Science, vol. 337, pp. 695–699, 2012.
  8. R. Lal, “World crop residues production and implications of its use as a biofuel,” Environment International, vol. 31, no. 4, pp. 575–584, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. C. Somerville, H. Youngs, C. Taylor, S. C. Davis, and S. P. Long, “Feedstocks for lignocellulosic biofuels,” Science, vol. 329, no. 5993, pp. 790–792, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. T. Vogt, “Phenylpropanoid biosynthesis,” Molecular Plant, vol. 3, no. 1, pp. 2–20, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. C. J. Liu, “Deciphering the enigma of lignification: precursor transport, oxidation, and the topochemistry of lignin assembly,” Molecular Plant, vol. 5, no. 2, pp. 304–317, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. N. Suzuki, S. Koussevitzky, R. Mittler, and G. Miller, “ROS and redox signalling in the response of plants to abiotic stress,” Plant, Cell and Environment, vol. 35, no. 2, pp. 259–270, 2012. View at Publisher · View at Google Scholar · View at Scopus
  13. L. Almagro, L. V. G. Ros, S. Belchi-Navarro, R. Bru, A. R. Barceló, and M. A. Pedreño, “Class III peroxidases in plant defence reactions,” Journal of Experimental Botany, vol. 60, no. 2, pp. 377–390, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. J. A. O'Brien, A. Daudi, V. S. Butt, and G. P. Bolwell, “Reactive oxygen species and their role in plant defence and cell wall metabolism,” Planta, vol. 236, pp. 765–779, 2012.
  15. K. Apel and H. Hirt, “Reactive oxygen species: metabolism, oxidative stress, and signal transduction,” Annual Review of Plant Biology, vol. 55, pp. 373–399, 2004. View at Publisher · View at Google Scholar · View at Scopus
  16. R. Vanholme, B. Demedts, K. Morreel, J. Ralph, and W. Boerjan, “Lignin biosynthesis and structure,” Plant Physiology, vol. 153, no. 3, pp. 895–905, 2010. View at Publisher · View at Google Scholar · View at Scopus
  17. N. C. Carpita, “Progress in the biological synthesis of the plant cell wall: new ideas for improving biomass for bioenergy,” Current Opinion in Biotechnology, vol. 23, pp. 330–337, 2011.
  18. R. S. Fukushima and R. D. Hatfield, “Comparison of the acetyl bromide spectrophotometric method with other analytical lignin methods for determining lignin concentration in forage samples,” Journal of Agricultural and Food Chemistry, vol. 52, no. 12, pp. 3713–3720, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. G. G. Allison, C. Morris, J. Clifton-Brown, S. J. Lister, and I. S. Donnison, “Genotypic variation in cell wall composition in a diverse set of 244 accessions of Miscanthus,” Biomass & Bioenergy, vol. 35, no. 11, pp. 4740–4747, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. C. E. Jahn, J. K. Mckay, R. Mauleon et al., “Genetic variation in biomass traits among 20 diverse rice varieties,” Plant Physiology, vol. 155, no. 1, pp. 157–168, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. M. Sakiroglu, K. J. Moore, and E. C. Brummer, “Variation in biomass yield, cell wall components, and agronomic traits in a broad range of diploid alfalfa accessions,” Crop Science, vol. 51, no. 5, pp. 1956–1964, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Kramer, M. R. Weisbjerg, P. Lund, C. S. Jensen, and M. G. Pedersen, “Estimation of indigestible NDF in forages and concentrates from cell wall composition,” Animal Feed Science and Technology, vol. 177, pp. 40–51, 2012.
  23. J. B. Lowry, L. L. Conlan, A. C. Schlink, and C. S. McSweeney, “Acid detergent dispersible lignin in tropical grasses,” Journal of the Science of Food and Agriculture, vol. 65, pp. 41–49, 1994.
  24. P. M. Schwab, J. F. S. Lamb, C. C. Sheaffer, and D. K. Barnes, “Germplasm variability and environmental effects on stem cellulose and lignin concentrations in Alfalfa,” Journal of Agronomy and Crop Science, vol. 191, no. 5, pp. 386–392, 2005. View at Publisher · View at Google Scholar · View at Scopus
  25. D. Wilman and P. R. Moghaddam, “In vitro digestibility and neutral detergent fibre and lignin contents of plant parts of nine forage species,” Journal of Agricultural Science, vol. 131, no. 1, pp. 51–58, 1998. View at Publisher · View at Google Scholar · View at Scopus
  26. K. E. B. Knudsen, “Carbohydrate and lignin contents of plant materials used in animal feeding,” Animal Feed Science and Technology, vol. 67, no. 4, pp. 319–338, 1997. View at Scopus
  27. A. D. Iwaasa, K. A. Beauchemin, J. G. Buchanan-Smith, and S. N. Acharya, “Effect of stage of maturity and growth cycle on shearing force and cell wall chemical constituents of alfalfa stems,” Canadian Journal of Animal Science, vol. 76, no. 3, pp. 321–328, 1996. View at Scopus
  28. D. G. Armstrong, H. Cook, and B. Thomas, “The lignin and cellulose contents of certain grassland species at different stages of growth,” Journal of Agricultural Science, vol. 40, pp. 93–99, 1950.
  29. G. G. McBee and F. R. Miller, “Stem carbohydrate and lignin concentrations in sorghum hybrids at 7 growth-stages,” Crop Science, vol. 33, pp. 530–534, 1993.
  30. Y. Arai-Sanoh, M. Ida, R. Zhao et al., “Genotypic variations in non-structural carbohydrate and cell-wall components of the stem in rice, sorghum, and sugar vane,” Bioscience, Biotechnology and Biochemistry, vol. 75, no. 6, pp. 1104–1112, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. S. Abiven, A. Heim, and M. W. I. Schmidt, “Lignin content and chemical characteristics in maize and wheat vary between plant organs and growth stages: consequences for assessing lignin dynamics in soil,” Plant and Soil, vol. 343, no. 1-2, pp. 369–378, 2011. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Frei, H. P. S. Makkar, K. Becker, and M. Wissuwa, “Ozone exposure during growth affects the feeding value of rice shoots,” Animal Feed Science and Technology, vol. 155, no. 1, pp. 74–79, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. Y. Wang and M. Frei, “Stressed food—the impact of abiotic environmental stresses on crop quality,” Agriculture, Ecosystems & Environment, vol. 141, no. 3-4, pp. 271–286, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Frei, Y. Kohno, M. Wissuwa, H. P. S. Makkar, and K. Becker, “Negative effects of tropospheric ozone on the feed value of rice straw are mitigated by an ozone tolerance QTL,” Global Change Biology, vol. 17, no. 7, pp. 2319–2329, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. R. Hatfield and R. S. Fukushima, “Can lignin be accurately measured?” Crop Science, vol. 45, no. 3, pp. 832–839, 2005. View at Publisher · View at Google Scholar · View at Scopus
  36. B. M. Goff, P. T. Murphy, and K. J. Moore, “Comparison of common lignin methods and modifications on forage and lignocellulosic biomass materials,” Journal of the Science of Food and Agriculture, vol. 92, no. 4, pp. 751–758, 2012. View at Publisher · View at Google Scholar · View at Scopus
  37. K. Brinkmann, L. Blaschke, and A. Polle, “Comparison of different methods for lignin determination as a basis for calibration of near-infrared reflectance spectroscopy and implications of lignoproteins,” Journal of Chemical Ecology, vol. 28, no. 12, pp. 2483–2501, 2002. View at Publisher · View at Google Scholar · View at Scopus
  38. R. R. Stange Jr. and R. E. McDonald, “A simple and rapid method for determination of lignin in plant tissues—its usefulness in elicitor screening and comparison to the thioglycolic acid method,” Postharvest Biology and Technology, vol. 15, no. 2, pp. 185–193, 1999. View at Publisher · View at Google Scholar · View at Scopus
  39. P. J. Vansoest, J. B. Robertson, and B. A. Lewis, “Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition,” Journal of Dairy Science, vol. 74, no. 10, pp. 3583–3597, 1991. View at Scopus
  40. K. H. Südekum, K. Voigt, B. Monties, and M. Stangassinger, “Spectrophotometric investigations on lignin in wheat (Triticum aestivum L.): influence of cell wall preparation, solvent, and standard,” Journal of Agricultural and Food Chemistry, vol. 45, no. 4, pp. 1220–1228, 1997. View at Scopus
  41. J. B. Sluiter, R. O. Ruiz, C. J. Scarlata, A. D. Sluiter, and D. W. Templeton, “Compositional analysis of lignocellulosic feedstocks. 1. Review and description of methods,” Journal of Agricultural and Food Chemistry, vol. 58, no. 16, pp. 9043–9053, 2010. View at Publisher · View at Google Scholar · View at Scopus
  42. S. Suzuki, Y. Suzuki, N. Yamamoto, T. Hattori, M. Sakamoto, and T. Umezawa, “High-throughput determination of thioglycolic acid lignin from rice,” Plant Biotechnology, vol. 26, no. 3, pp. 337–340, 2009. View at Scopus
  43. R. S. Fukushima and B. A. Dehority, “Feasibility of using lignin isolated from forages by solubilization in acetyl bromide as a standard for lignin analyses,” Journal of Animal Science, vol. 78, no. 12, pp. 3135–3143, 2000. View at Scopus
  44. C. Huang, L. Han, X. Liu, and L. Ma, “The rapid estimation of cellulose, hemicellulose, and lignin contents in rice straw by near infrared spectroscopy,” Energy Sources A, vol. 33, no. 2, pp. 114–120, 2011. View at Publisher · View at Google Scholar · View at Scopus
  45. H. J. G. Jung, “Analysis of forage fiber and cell walls in ruminant nutrition,” Journal of Nutrition, vol. 127, supplement 5, pp. S810–S813, 1997. View at Scopus
  46. J. F. Pedersen, K. P. Vogel, and D. L. Funnell, “Impact of reduced lignin on plant fitness,” Crop Science, vol. 45, no. 3, pp. 812–819, 2005. View at Publisher · View at Google Scholar · View at Scopus
  47. M. D. Casler, D. R. Buxton, and K. P. Vogel, “Genetic modification of lignin concentration affects fitness of perennial herbaceous plants,” Theoretical and Applied Genetics, vol. 104, no. 1, pp. 127–131, 2002. View at Publisher · View at Google Scholar · View at Scopus
  48. B. R. Lee, K. Y. Kim, W. J. Jung, J. C. Avice, A. Ourry, and T. H. Kim, “Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.),” Journal of Experimental Botany, vol. 58, no. 6, pp. 1271–1279, 2007. View at Publisher · View at Google Scholar · View at Scopus
  49. P. R. Peterson, C. C. Sheaffer, and M. H. Hall, “Drought effects on perennial forage legume yield and quality,” Agronomy Journal, vol. 84, pp. 774–779, 1992.
  50. H. V. Petit, A. R. Pesant, G. M. Barnett, W. N. Mason, and J. L. Dionne, “Quality and morphological-characteristics of alfalfa as affected by soil-moisture, ph and phosphorus fertilization,” Canadian Journal of Plant Science, vol. 72, pp. 147–162, 1992.
  51. D. A. Deetz, H. G. Jung, and D. R. Buxton, “Water-deficit effects on cell-wall composition and in vitro degradability of structural polysaccharides from alfalfa stems,” Crop Science, vol. 36, no. 2, pp. 383–388, 1996. View at Scopus
  52. M. L. Fiasconaro, Y. Gogorcena, F. Munoz, D. Andueza, M. Sanchez-Diaz, and M. C. Antolin, “Effects of nitrogen source and water availability on stem carbohydrates and cellulosic bioethanol traits of alfalfa plants,” Plant Science, vol. 191, pp. 16–23, 2012.
  53. B. R. Lee, S. Muneer, W. J. Jung, J. C. Avice, A. Ourry, and T.-H. Kim, “Mycorrhizal colonization alleviates drought-induced oxidative damage and lignification in the leaves of drought-stressed perennial ryegrass (Lolium perenne),” Physiologia Plantarum, vol. 145, pp. 440–449, 2012. View at Publisher · View at Google Scholar · View at Scopus
  54. O. Guenni, D. Marín, and Z. Baruch, “Responses to drought of five Brachiaria species. I. Biomass production, leaf growth, root distribution, water use and forage quality,” Plant and Soil, vol. 243, no. 2, pp. 229–241, 2002. View at Publisher · View at Google Scholar · View at Scopus
  55. Y. Jiang, Y. Yao, and Y. Wang, “Physiological response, cell wall components, and gene expression of switchgrass under short-term drought stress and recovery,” Crop Science, vol. 52, pp. 2718–2727, 2012.
  56. Y. Hu, W. C. Li, Y. Q. Xu, G. J. Li, Y. Liao, and F. L. Fu, “Differential expression of candidate genes for lignin biosynthesis under drought stress in maize leaves,” Journal of Applied Genetics, vol. 50, no. 3, pp. 213–223, 2009. View at Scopus
  57. D. Vincent, C. Lapierre, B. Pollet, G. Cornic, L. Negroni, and M. Zivy, “Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves. A proteomic investigation,” Plant Physiology, vol. 137, no. 3, pp. 949–960, 2005. View at Publisher · View at Google Scholar · View at Scopus
  58. V. Leinhos and H. Bergmann, “Changes in the yield, lignin content and protein patterns of barley (Hordeum vulgare cv Alexis) induced by drought stress,” Journal of Applied Botany, vol. 69, pp. 206–210, 1995.
  59. H. Mahmoudi, J. Huang, M. Y. Gruber et al., “The impact of genotype and salinity on physiological function, secondary metabolite accumulation, and antioxidative responses in lettuce,” Journal of Agricultural and Food Chemistry, vol. 58, no. 8, pp. 5122–5130, 2010. View at Publisher · View at Google Scholar · View at Scopus
  60. I. Sánchez-Aguayo, J. M. Rodríguez-Galán, R. García, J. Torreblanca, and J. M. Pardo, “Salt stress enhances xylem development and expression of S-adenosyl-L-methionine synthase in lignifying tissues of tomato plants,” Planta, vol. 220, no. 2, pp. 278–285, 2004. View at Publisher · View at Google Scholar · View at Scopus
  61. G. Peyrano, E. Taleisnik, M. Quiroga, S. M. de Forchetti, and H. Tigier, “Salinity effects on hydraulic conductance, lignin content and peroxidase activity in tomato roots,” Plant Physiology and Biochemistry, vol. 35, no. 5, pp. 387–393, 1997. View at Scopus
  62. P. Cachorro, A. Ortiz, A. R. Barcelo, and A. Cerda, “Lignin deposition in vascular tissues of phaseolus-ulgaris roots in response to salt stress and CA2+ ions,” Phyton-Annales Rei Botanicae, vol. 33, pp. 33–40, 1993.
  63. I. Karahara, A. Ikeda, T. Kondo, and Y. Uetake, “Development of the Casparian strip in primary roots of maize under salt stress,” Planta, vol. 219, no. 1, pp. 41–47, 2004. View at Publisher · View at Google Scholar · View at Scopus
  64. H. M. Wang, L. Zhou, Y. P. Fu et al., “Expression of an apoplast-localized BURP-domain protein from soybean (GmRD22) enhances tolerance towards abiotic stress,” Plant Cell and Environment, vol. 35, pp. 1932–1947, 2012.
  65. C. C. Lin and C. H. Kao, “Cell wall peroxidase against ferulic acid, lignin, and NaCl-reduced root growth of rice seedlings,” Journal of Plant Physiology, vol. 158, no. 5, pp. 667–671, 2001. View at Scopus
  66. G. Y. S. Neves, R. Marchiosi, M. L. L. Ferrarese, R. C. Siqueira-Soares, and O. Ferrarese-Filho, “Root growth inhibition and lignification induced by salt stress in soybean,” Journal of Agronomy and Crop Science, vol. 196, no. 6, pp. 467–473, 2010. View at Publisher · View at Google Scholar · View at Scopus
  67. J. Kováčik, F. Štork, B. Klejdus, J. Grúz, and J. Hedbavny, “Effect of metabolic regulators on aluminium uptake and toxicity in Matricaria chamomilla plants,” Plant Physiology and Biochemistry, vol. 54, pp. 140–148, 2012. View at Publisher · View at Google Scholar · View at Scopus
  68. B. Ma, L. Gao, H. Zhang, J. Cui, and Z. Shen, “Aluminum-induced oxidative stress and changes in antioxidant defenses in the roots of rice varieties differing in Al tolerance,” Plant Cell Reports, vol. 31, no. 4, pp. 687–696, 2012. View at Publisher · View at Google Scholar · View at Scopus
  69. M. A. Hossain, A. K. M. Z. Hossain, T. Kihara, H. Koyama, and T. Hara, “Aluminum-induced lipid peroxidation and lignin deposition are associated with an increase in H2O2 generation in wheat seedlings,” Soil Science and Plant Nutrition, vol. 51, no. 2, pp. 223–230, 2005. View at Scopus
  70. M. Sasaki, Y. Yamamoto, and H. Matsumoto, “Lignin deposition induced by aluminum in wheat (Triticum aestivum) roots,” Physiologia Plantarum, vol. 96, no. 2, pp. 193–198, 1996. View at Scopus
  71. M. D. Heidarabadi, F. Ghanati, and T. Fujiwara, “Interaction between boron and aluminum and their effects on phenolic metabolism of Linum usitatissimum L. roots,” Plant Physiology and Biochemistry, vol. 49, no. 12, pp. 1377–1383, 2011. View at Publisher · View at Google Scholar · View at Scopus
  72. A. Finger-Teixeira, M. D. L. Lucio Ferrarese, A. R. Soares, D. da Silva, and O. Ferrarese-Filho, “Cadmium-induced lignification restricts soybean root growth,” Ecotoxicology and Environmental Safety, vol. 73, no. 8, pp. 1959–1964, 2010. View at Publisher · View at Google Scholar · View at Scopus
  73. C. C. Lin, L. M. Chen, and Z. H. Liu, “Rapid effect of copper on lignin biosynthesis in soybean roots,” Plant Science, vol. 168, no. 3, pp. 855–861, 2005. View at Publisher · View at Google Scholar · View at Scopus
  74. L. M. Cervilla, M. A. Rosales, M. M. Rubio-Wilhelmi et al., “Involvement of lignification and membrane permeability in the tomato root response to boron toxicity,” Plant Science, vol. 176, no. 4, pp. 545–552, 2009. View at Publisher · View at Google Scholar · View at Scopus
  75. P. H. Brown, R. D. Graham, and D. J. D. Nicholas, “The effects of manganese and nitrate supply on the levels of phenolics and lignin in young wheat plants,” Plant and Soil, vol. 81, pp. 437–440, 1984.
  76. Z. Rengel, R. D. Graham, and J. F. Pedler, “Time-course of biosynthesis of phenolics and lignin in roots of wheat genotypes differing in manganese efficiency and resistance to take-all fungus,” Annals of Botany, vol. 74, no. 5, pp. 471–477, 1994. View at Publisher · View at Google Scholar · View at Scopus
  77. J. Kováčik, B. Klejdus, F. Štork, and J. Hedbavny, “Nitrate deficiency reduces cadmium and nickel accumulation in chamomile plants,” Journal of Agricultural and Food Chemistry, vol. 59, no. 9, pp. 5139–5149, 2011. View at Publisher · View at Google Scholar · View at Scopus
  78. C. Fritz, N. Palacios-Rojas, R. Feil, and M. Stitt, “Regulation of secondary metabolism by the carbon-nitrogen status in tobacco: nitrate inhibits large sectors of phenylpropanoid metabolism,” Plant Journal, vol. 46, no. 4, pp. 533–548, 2006. View at Publisher · View at Google Scholar · View at Scopus
  79. A. F. Teixeira, A. D. B. Andrade, O. Ferrarese-Filho, and M. D. L. Ferrarese, “Role of calcium on phenolic compounds and enzymes related to lignification in soybean (Glycine max L.) root growth,” Plant Growth Regulation, vol. 49, no. 1, pp. 69–76, 2006. View at Publisher · View at Google Scholar · View at Scopus
  80. W. H. Eppendorfer and B. O. Eggum, “Effects of sulphur, nitrogen, phosphorus, potassium, and water stress on dietary fibre fractions, starch, amino acids and on the biological value of potato protein,” Plant Foods for Human Nutrition, vol. 45, no. 4, pp. 299–313, 1994. View at Publisher · View at Google Scholar · View at Scopus
  81. S. Suzuki, J. F. Ma, N. Yamamoto, T. Hattori, M. Sakamoto, and T. Umezawa, “Silicon deficiency promotes lignin accumulation in rice,” Plant Biotechnology, vol. 29, pp. 391–394, 2012.
  82. M. Frei, M. Wissuwa, J. Pariasca-Tanaka, C. P. Chen, K.-H. Südekum, and Y. Kohno, “Leaf ascorbic acid level—is it really important for ozone tolerance in rice?” Plant Physiology and Biochemistry, vol. 59, pp. 63–70, 2012. View at Publisher · View at Google Scholar · View at Scopus
  83. J. Sanz, R. B. Muntifering, V. Bermejo, B. S. Gimeno, and S. Elvira, “Ozone and increased nitrogen supply effects on the yield and nutritive quality of Trifolium subterraneum,” Atmospheric Environment, vol. 39, no. 32, pp. 5899–5907, 2005. View at Publisher · View at Google Scholar · View at Scopus
  84. R. B. Muntifering, A. H. Chappelka, J. C. Lin, D. F. Karnosky, and G. L. Somers, “Chemical composition and digestibility of Trifolium exposed to elevated ozone and carbon dioxide in a free-air (FACE) fumigation system,” Functional Ecology, vol. 20, no. 2, pp. 269–275, 2006. View at Publisher · View at Google Scholar · View at Scopus
  85. R. B. Muntifering, D. D. Crosby, M. C. Powell, and A. H. Chappelka, “Yield and quality characteristics of bahiagrass (Paspalum notatum) exposed to ground-level ozone,” Animal Feed Science and Technology, vol. 84, no. 3-4, pp. 243–256, 2000. View at Publisher · View at Google Scholar · View at Scopus
  86. M. C. Powell, R. B. Muntifering, J. C. Lin, and A. H. Chappelka, “Yield and nutritive quality of sericea lespedeza (Lespedeza cuneata) and little bluestem (Schizachyrium scoparium) exposed to ground-level ozone,” Environmental Pollution, vol. 122, no. 3, pp. 313–322, 2003. View at Publisher · View at Google Scholar · View at Scopus
  87. J. Bender, R. B. Muntifering, J. C. Lin, and H. J. Weigel, “Growth and nutritive quality of Poa pratensis as influenced by ozone and competition,” Environmental Pollution, vol. 142, no. 1, pp. 109–115, 2006. View at Publisher · View at Google Scholar · View at Scopus
  88. J. Rozema, M. Tosserams, H. J. M. Nelissen, L. van Heerwaarden, R. A. Broekman, and N. Flierman, “Stratospheric ozone reduction and ecosystem processes: enhanced UV-B radiation affects chemical quality and decomposition of leaves of the dune grassland species Calamagrostis epigeios,” Plant Ecology, vol. 128, no. 1-2, pp. 284–294, 1997. View at Scopus
  89. M. T. Charles, A. Goulet, and J. Arul, “Physiological basis of UV-C induced resistance to Botrytis cinerea in tomato fruit. IV. Biochemical modification of structural barriers,” Postharvest Biology and Technology, vol. 47, no. 1, pp. 41–53, 2008. View at Publisher · View at Google Scholar · View at Scopus
  90. S. Yamasaki, N. Noguchi, and K. Mimaki, “Continuous UV-B irradiation induces morphological changes and the accumulation of polyphenolic compounds on the surface of cucumber cotyledons,” Journal of Radiation Research, vol. 48, no. 6, pp. 443–454, 2007. View at Publisher · View at Google Scholar · View at Scopus
  91. M. Hilal, M. F. Parrado, M. Rosa et al., “Epidermal lignin deposition in quinoa cotyledons in response to UV-B radiation,” Photochemistry and Photobiology, vol. 79, pp. 205–210, 2004.
  92. J. A. Zavala, A. L. Scopel, and C. L. Ballaré, “Effects of ambient UV-B radiation on soybean crops: impact on leaf herbivory by Anticarsia gemmatalis,” Plant Ecology, vol. 156, no. 2, pp. 121–130, 2001. View at Publisher · View at Google Scholar · View at Scopus
  93. A.-R. Ballester, M. T. Lafuente, J. Forment et al., “Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance,” Molecular Plant Pathology, vol. 12, no. 9, pp. 879–897, 2011. View at Publisher · View at Google Scholar · View at Scopus
  94. M. C. Valentines, R. Vilaplana, R. Torres, J. Usall, and C. Larrigaudière, “Specific roles of enzymatic browning and lignification in apple disease resistance,” Postharvest Biology and Technology, vol. 36, no. 3, pp. 227–234, 2005. View at Publisher · View at Google Scholar · View at Scopus
  95. N. H. Bhuiyan, G. Selvaraj, Y. Wei, and J. King, “Role of lignification in plant defense,” Plant Signaling & Behavior, vol. 4, no. 2, pp. 158–159, 2009. View at Scopus
  96. N. H. Bhuiyan, G. Selvaraj, Y. Wei, and J. King, “Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion,” Journal of Experimental Botany, vol. 60, no. 2, pp. 509–521, 2009. View at Publisher · View at Google Scholar · View at Scopus
  97. C. Bi, F. Chen, L. Jackson, B. S. Gill, and W. Li, “Expression of lignin biosynthetic genes in wheat during development and upon infection by fungal pathogens,” Plant Molecular Biology Reporter, vol. 29, no. 1, pp. 149–161, 2011. View at Publisher · View at Google Scholar · View at Scopus
  98. L. G. Dushnicky, G. M. Ballance, M. J. Sumner, and A. W. MacGregor, “The role of lignification as a resistance mechanism in wheat to a toxin-producing isolate of Pyrenophora tritici-repentis,” Canadian Journal of Plant Pathology, vol. 20, no. 1, pp. 35–47, 1998. View at Scopus
  99. H. Wang, J. Hao, X. Chen et al., “Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants,” Plant Molecular Biology, vol. 65, no. 6, pp. 799–815, 2007. View at Publisher · View at Google Scholar · View at Scopus
  100. W. Sun, J. Zhang, Q. Fan, G. Xue, Z. Li, and Y. Liang, “Silicon-enhanced resistance to rice blast is attributed to silicon-mediated defence resistance and its role as physical barrier,” European Journal of Plant Pathology, vol. 128, no. 1, pp. 39–49, 2010. View at Publisher · View at Google Scholar · View at Scopus
  101. P. M. Dracatos, N. O. I. Cogan, M. P. Dobrowolski et al., “Discovery and genetic mapping of single nucleotide polymorphisms in candidate genes for pathogen defence response in perennial ryegrass (Lolium perenne L.),” Theoretical and Applied Genetics, vol. 117, no. 2, pp. 203–219, 2008. View at Publisher · View at Google Scholar · View at Scopus
  102. C. Eynck, G. Séguin-Swartz, W. E. Clarke, and I. A. P. Parkin, “Monolignol biosynthesis is associated with resistance to Sclerotinia sclerotiorum in Camelina sativa,” Molecular Plant Pathology, vol. 13, pp. 887–899, 2012. View at Publisher · View at Google Scholar · View at Scopus
  103. S. Guillaumie, R. Mzid, V. Méchin et al., “The grapevine transcription factor WRKY2 influences the lignin pathway and xylem development in tobacco,” Plant Molecular Biology, vol. 72, no. 1-2, pp. 215–234, 2010. View at Publisher · View at Google Scholar · View at Scopus
  104. R. Mzid, C. Marchive, D. Blancard et al., “Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens,” Physiologia Plantarum, vol. 131, no. 3, pp. 434–447, 2007. View at Publisher · View at Google Scholar · View at Scopus
  105. L. G. Kamphuis, A. H. Williams, H. Küster et al., “Phoma medicaginis stimulates the induction of the octadecanoid and phenylpropanoid pathways in Medicago truncatula,” Molecular Plant Pathology, vol. 13, pp. 593–603, 2012. View at Publisher · View at Google Scholar · View at Scopus
  106. M. Kozlowska and Z. Krzywanski, “Lignification in red raspberry canes upon wounding and fungal infection,” Acta Physiologiae Plantarum, vol. 13, pp. 115–121, 1991.
  107. A. V. Lygin, S. Li, R. Vittal, J. M. Widholm, G. L. Hartman, and V. V. Lozovaya, “The importance of phenolic metabolism to limit the growth of Phakopsora pachyrhizi,” Phytopathology, vol. 99, no. 12, pp. 1412–1420, 2009. View at Publisher · View at Google Scholar · View at Scopus
  108. H. Shi, Z. Liu, L. Zhu et al., “Overexpression of cotton (Gossypium hirsutum) dirigent1 gene enhances lignification that blocks the spread of Verticillium dahliae,” Acta Biochimica et Biophysica Sinica, vol. 44, pp. 555–564, 2012.
  109. K. A. M. Abo-Elyousr, M. Hashem, and E. H. Ali, “Integrated control of cotton root rot disease by mixing fungal biocontrol agents and resistance inducers,” Crop Protection, vol. 28, no. 4, pp. 295–301, 2009. View at Publisher · View at Google Scholar · View at Scopus
  110. L. Xu, L. Zhu, L. Tu et al., “Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry,” Journal of Experimental Botany, vol. 62, no. 15, pp. 5607–5621, 2011. View at Publisher · View at Google Scholar · View at Scopus
  111. G. S. Wu, B. J. Shortt, E. B. Lawrence et al., “Activation of host defense mechanisms by elevated production of H2O2 in transgenic plants,” Plant Physiology, vol. 115, no. 2, pp. 427–435, 1997. View at Scopus
  112. A. B. Andreu, M. G. Guevara, E. A. Wolski, G. R. Daleo, and D. O. Caldiz, “Enhancement of natural disease resistance in potatoes by chemicals,” Pest Management Science, vol. 62, no. 2, pp. 162–170, 2006. View at Publisher · View at Google Scholar · View at Scopus
  113. O. Wally and Z. K. Punja, “Enhanced disease resistance in transgenic carrot (Daucus carota L.) plants over-expressing a rice cationic peroxidase,” Planta, vol. 232, no. 5, pp. 1229–1239, 2010. View at Publisher · View at Google Scholar · View at Scopus
  114. B. Garrod, R. G. Lewis, M. J. Brittain, and W. P. Davies, “Studies on the contribution of lignin and suberin to the impedance of wounded carrot root-tissue to fungal invasion,” New Phytologist, vol. 90, pp. 99–108, 1982.
  115. S. Mandal and A. Mitra, “Reinforcement of cell wall in roots of Lycopersicon esculentum through induction of phenolic compounds and lignin by elicitors,” Physiological and Molecular Plant Pathology, vol. 71, no. 4–6, pp. 201–209, 2007. View at Publisher · View at Google Scholar · View at Scopus
  116. K. C. Nagarathna, S. A. Shetty, and H. S. Shetty, “Phenylalanine ammonia lyase activity in pearl millet seedlings and its relation to downy mildew disease resistance,” Journal of Experimental Botany, vol. 44, no. 8, pp. 1291–1296, 1993. View at Publisher · View at Google Scholar · View at Scopus
  117. D. Nandini, J. S. S. Mohan, and G. Singh, “Induction of systemic acquired resistance in arachis hypogaea l. by sclerotium rolfsii derived elicitors,” Journal of Phytopathology, vol. 158, no. 9, pp. 594–600, 2010. View at Publisher · View at Google Scholar · View at Scopus
  118. F. Pomar, M. Novo, M. A. Bernal, F. Merino, and A. R. Barceló, “Changes in stem lignins (monomer composition and crosslinking) and peroxidase are related with the maintenance of leaf photosynthetic integrity during Verticillium wilt in Capsicum annuum,” New Phytologist, vol. 163, no. 1, pp. 111–123, 2004. View at Publisher · View at Google Scholar · View at Scopus
  119. J. Siegrist, W. Jeblick, and H. Kauss, “Defense responses in infected and elicited cucumber (Cucumis sativus L.) hypocotyl segments exhibiting acquired resistance,” Plant Physiology, vol. 105, no. 4, pp. 1365–1374, 1994. View at Scopus
  120. P. Vidhyasekaran, N. Kamala, A. Ramanathan, K. Rajappan, V. Paranidharan, and R. Velazhahan, “Induction of systemic resistance by Pseudomonas fluorescens Pf1 against Xanthomonas oryzae pv. Oryzae in rice leaves,” Phytoparasitica, vol. 29, no. 2, pp. 155–166, 2001. View at Scopus
  121. M. Elfstrand, F. Sitbon, C. Lapierre, A. Bottin, and S. von Arnold, “Altered lignin structure and resistance to pathogens in spi 2-expressing tobacco plants,” Planta, vol. 214, no. 5, pp. 708–716, 2002. View at Publisher · View at Google Scholar · View at Scopus
  122. T. Ishihara, I. Mitsuhara, H. Takahashi, and K. Nakaho, “Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato,” PLoS ONE, vol. 7, no. 10, Article ID e46763, 2012.
  123. R. Fogain and S. R. Gowen, “Investigations on possible mechanisms of resistance to nematodes in Musa,” Euphytica, vol. 92, no. 3, pp. 375–381, 1996. View at Scopus
  124. R. S. Kavitha, T. N. Balamohan, M. Kavitha, and B. S. Selvi, “Biochemical interactions of banana hybrids to root lesion nematodes (Pratylenchus coffeae),” Plant Archives, vol. 8, pp. 105–110, 2008.
  125. C. Valette, C. Andary, J. P. Geiger, J. L. Sarah, and M. Nicole, “Histochemical and cytochemical investigations of phenols in roots of banana infected by the burrowing nematode Radopholus similis,” Phytopathology, vol. 88, no. 11, pp. 1141–1148, 1998. View at Scopus
  126. G. Zacheo, T. Bleve-Zacheo, D. Pacoda, C. Orlando, and R. D. Durbin, “The association between heat-induced susceptibility of tomato to Meloidogyne incognita and peroxidase activity,” Physiological and Molecular Plant Pathology, vol. 46, no. 6, pp. 491–507, 1995. View at Publisher · View at Google Scholar · View at Scopus
  127. N. Ithal, J. Recknor, D. Nettleton et al., “Parallel genome-wide expression profiling of host and pathogen during soybean cyst nematode infection of soybean,” Molecular Plant-Microbe Interactions, vol. 20, no. 3, pp. 293–305, 2007. View at Publisher · View at Google Scholar · View at Scopus
  128. J. Barros-Rios, R. A. Malvar, H.-J. G. Jung, and R. Santiago, “Cell wall composition as a maize defense mechanism against corn borers,” Phytochemistry, vol. 72, no. 4-5, pp. 365–371, 2011. View at Publisher · View at Google Scholar · View at Scopus
  129. H. H. Beeghly, J. G. Coors, and M. Lee, “Plant fiber composition and resistance to European corn borer in four maize populations,” Maydica, vol. 42, no. 3, pp. 297–303, 1997. View at Scopus
  130. S. N. Johnson, P. D. Hallett, T. L. Gillespie, and C. Halpin, “Below-ground herbivory and root toughness: a potential model system using lignin-modified tobacco,” Physiological Entomology, vol. 35, no. 2, pp. 186–191, 2010. View at Publisher · View at Google Scholar · View at Scopus
  131. J. Loranger, S. T. Meyer, B. Shipley et al., “Predicting invertebrate herbivory from plant traits: evidence from 51 grassland species in experimental monocultures,” Ecology, vol. 93, pp. 2674–2682, 2012.
  132. V. Méchin, O. Argillier, Y. Hébert et al., “Genetic analysis and QTL mapping of cell wall digestibility and lignification in silage maize,” Crop Science, vol. 41, no. 3, pp. 690–697, 2001. View at Scopus
  133. V. Roussel, C. Gibelin, A. S. Fontaine, and Y. Barrière, “Genetic analysis in recombinant inbred lines of early dent forage maize. II—QTL mapping for cell wall constituents and cell wall digestibility from per se value and top cross experiments,” Maydica, vol. 47, no. 1, pp. 9–20, 2002. View at Scopus
  134. A. J. Cardinal, M. Lee, and K. J. Moore, “Genetic mapping and analysis of quantitative trait loci affecting fiber and lignin content in maize,” Theoretical and Applied Genetics, vol. 106, no. 5, pp. 866–874, 2003. View at Scopus
  135. M. D. Krakowsky, M. Lee, and J. G. Coors, “Quantitative trait loci for cell-wall components in recombinant inbred lines of maize (Zea mays L.) I: stalk tissue,” Theoretical and Applied Genetics, vol. 111, no. 2, pp. 337–346, 2005. View at Publisher · View at Google Scholar · View at Scopus
  136. M. D. Krakowsky, M. Lee, and J. G. Coors, “Quantitative trait loci for cell wall components in recombinant inbred lines of maize (Zea mays L.) II: leaf sheath tissue,” Theoretical and Applied Genetics, vol. 112, no. 4, pp. 717–726, 2006. View at Publisher · View at Google Scholar · View at Scopus
  137. Y. Barrière, J. Thomas, and D. Denoue, “QTL mapping for lignin content, lignin monomeric composition, p-hydroxycinnamate content, and cell wall digestibility in the maize recombinant inbred line progeny F838 × F286,” Plant Science, vol. 175, no. 4, pp. 585–595, 2008. View at Publisher · View at Google Scholar · View at Scopus
  138. C. Riboulet, F. Fabre, D. Dénoue, J. P. Martinantä, B. Lefèvre, and Y. Barrière, “QTL mapping and candidate gene research for lignin content and cell wall digestibility in a top-cross of a flint maize recombinant inbred line progeny harvested at silage stage,” Maydica, vol. 53, no. 1, pp. 1–9, 2008. View at Scopus
  139. Y. Barrière, V. Méchin, D. Denoue, C. Bauland, and J. Laborde, “QTL for yield, earliness, and cell wall quality traits in topcross experiments of the F838 × F286 early maize RIL progeny,” Crop Science, vol. 50, no. 5, pp. 1761–1772, 2010. View at Publisher · View at Google Scholar · View at Scopus
  140. R. E. Lorenzana, M. F. Lewis, H.-J. G. Jung, and R. Bernardo, “Quantitative trait loci and trait correlations for maize stover cell wall composition and glucose release for cellulosic ethanol,” Crop Science, vol. 50, no. 2, pp. 541–555, 2010. View at Publisher · View at Google Scholar · View at Scopus
  141. A. J. Lorenz, J. G. Coors, C. N. Hansey, S. M. Kaeppler, and N. de Leon, “Genetic analysis of cell wall traits relevant to cellulosic ethanol production in maize (Zea mays L.),” Crop Science, vol. 50, no. 3, pp. 842–852, 2010. View at Publisher · View at Google Scholar · View at Scopus
  142. Y. Barrière, V. Méchin, B. Lefevre, and S. Maltese, “QTLs for agronomic and cell wall traits in a maize RIL progeny derived from a cross between an old Minnesota13 line and a modern Iodent line,” Theoretical and Applied Genetics, vol. 125, pp. 531–549, 2012. View at Publisher · View at Google Scholar · View at Scopus
  143. S. Grando, M. Baum, S. Ceccarelli et al., “QTLs for straw quality characteristics identified in recombinant inbred lines of a Hordeum vulgare × H. spontaneum cross in a Mediterranean environment,” Theoretical and Applied Genetics, vol. 110, no. 4, pp. 688–695, 2005. View at Publisher · View at Google Scholar · View at Scopus
  144. B. A. Siahsar, S. A. Peighambari, A. R. Taleii, M. R. Naghavi, A. Nabipour, and A. Sarrafi, “QTL analysis of forage quality traits in barley (Hordeum vulgare L.),” Cereal Research Communications, vol. 37, pp. 479–488, 2009.
  145. S. C. Murray, W. L. Rooney, S. E. Mitchell et al., “Genetic improvement of sorghum as a biofuel feedstock: II. QTL for stem and leaf structural carbohydrates,” Crop Science, vol. 48, no. 6, pp. 2180–2193, 2008. View at Publisher · View at Google Scholar · View at Scopus
  146. A. L. Shiringani and W. Friedt, “QTL for fibre-related traits in grain × sweet sorghum as a tool for the enhancement of sorghum as a biomass crop,” Theoretical and Applied Genetics, vol. 123, no. 6, pp. 999–1011, 2011. View at Scopus
  147. J. S. Bao, L. Jin, Y. Shen, and J. K. Xie, “Genetic mapping of quantitative trait loci associated with fiber and lignin content in rice,” Cereal Research Communications, vol. 35, no. 1, pp. 23–30, 2007. View at Publisher · View at Google Scholar · View at Scopus
  148. J.-K. Xie, X.-L. Kong, J. Chen et al., “Mapping of quantitative trait loci for fiber and lignin contents from an interspecific cross Oryza sativa × Oryza rufipogon,” Journal of Zhejiang University B, vol. 12, no. 7, pp. 518–526, 2011. View at Publisher · View at Google Scholar · View at Scopus
  149. L. Liu, A. Stein, B. Wittkop et al., “A knockout mutation in the lignin biosynthesis gene CCR1 explains a major QTL for acid detergent lignin content in Brassica napus seeds,” Theoretical and Applied Genetics, vol. 124, pp. 1573–1586, 2012. View at Publisher · View at Google Scholar · View at Scopus
  150. W. Vermerris, D. M. Sherman, and L. M. McIntyre, “Phenotypic plasticity in cell walls of maize brown midrib mutants is limited by lignin composition,” Journal of Experimental Botany, vol. 61, no. 9, pp. 2479–2490, 2010. View at Publisher · View at Google Scholar · View at Scopus
  151. Y.-H. Kim, C. Y. Kim, W.-K. Song et al., “Overexpression of sweetpotato swpa4 peroxidase results in increased hydrogen peroxide production and enhances stress tolerance in tobacco,” Planta, vol. 227, no. 4, pp. 867–881, 2008. View at Publisher · View at Google Scholar · View at Scopus
  152. L. Gallego-Giraldo, Y. Jikumaru, Y. Kamiya, Y. Tang, and R. A. Dixon, “Selective lignin downregulation leads to constitutive defense response expression in alfalfa (Medicago sativa L.),” New Phytologist, vol. 190, no. 3, pp. 627–639, 2011. View at Publisher · View at Google Scholar · View at Scopus
  153. L. Schreiber, “Chemical composition of Casparian strips isolated from Clivia miniata Reg. roots: evidence for lignin,” Planta, vol. 199, no. 4, pp. 596–601, 1996. View at Scopus
  154. E. Hose, D. T. Clarkson, E. Steudle, L. Schreiber, and W. Hartung, “The exodermis: a variable apoplastic barrier,” Journal of Experimental Botany, vol. 52, no. 365, pp. 2245–2264, 2001. View at Scopus
  155. S. Naseer, Y. Lee, C. Lapierre, R. Franke, C. Nawrath, and N. Geldner, “Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberin,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, pp. 10101–10106, 2012.
  156. J. Kovacik, B. Klejdus, J. Hedbavny, F. Stork, and J. Gruz, “Modulation of copper uptake and toxicity by abiotic stresses in Matricaria chamomilla plants,” Journal of Agricultural and Food Chemistry, vol. 60, pp. 6755–6763, 2012.
  157. J. E. van de Mortel, L. A. Villanueva, H. Schat et al., “Large expression differences in genes for iron and zinc homeostasis, stress response, and lignin biosynthesis distinguish roots of Arabidopsis thaliana and the related metal hyperaccumulator Thlaspi caerulescens,” Plant Physiology, vol. 142, no. 3, pp. 1127–1147, 2006. View at Publisher · View at Google Scholar · View at Scopus
  158. H. Matsumoto and H. Motoda, “Aluminum toxicity recovery processes in root apices. Possible association with oxidative stress,” Plant Science, vol. 185–186, pp. 1–8, 2012. View at Publisher · View at Google Scholar · View at Scopus
  159. C. Dordas, “Role of nutrients in controlling plant diseases in sustainable agriculture. A review,” Agronomy for Sustainable Development, vol. 28, no. 1, pp. 33–46, 2008. View at Publisher · View at Google Scholar · View at Scopus
  160. B. R. Hames, B. Kurek, B. Pollet, C. Lapierre, and B. Monties, “Interaction between MnO2 and oxalate: formation of a natural and abiotic lignin oxidizing system,” Journal of Agricultural and Food Chemistry, vol. 46, no. 12, pp. 5362–5367, 1998. View at Scopus
  161. M. Baier, A. Kandlbinder, D. Golldack, and K. J. Dietz, “Oxidative stress and ozone: perception, signalling and response,” Plant, Cell and Environment, vol. 28, no. 8, pp. 1012–1020, 2005. View at Publisher · View at Google Scholar · View at Scopus
  162. E. L. Fiscus, F. L. Booker, and K. O. Burkey, “Crop responses to ozone: uptake, modes of action, carbon assimilation and partitioning,” Plant, Cell and Environment, vol. 28, no. 8, pp. 997–1011, 2005. View at Publisher · View at Google Scholar · View at Scopus
  163. J. Kangasjärvi, P. Jaspers, and H. Kollist, “Signalling and cell death in ozone-exposed plants,” Plant, Cell and Environment, vol. 28, no. 8, pp. 1021–1036, 2005. View at Publisher · View at Google Scholar · View at Scopus
  164. M. Frei, J. P. Tanaka, C. P. Chen, and M. Wissuwa, “Mechanisms of ozone tolerance in rice: characterization of two QTLs affecting leaf bronzing by gene expression profiling and biochemical analyses,” Journal of Experimental Botany, vol. 61, no. 5, pp. 1405–1417, 2010. View at Publisher · View at Google Scholar · View at Scopus
  165. L. Guidi, E. Degl'Innocenti, S. Genovesi, and G. F. Soldatini, “Photosynthetic process and activities of enzymes involved in the phenylpropanoid pathway in resistant and sensitive genotypes of Lycopersicon esculentum L. exposed to ozone,” Plant Science, vol. 168, no. 1, pp. 153–160, 2005. View at Publisher · View at Google Scholar · View at Scopus
  166. J. Davidson and M. Phillips, “Lignin as a possible factor in lodging of cereals,” Science, vol. 72, no. 1868, pp. 401–402, 1930. View at Scopus
  167. Q.-H. Ma, “Functional analysis of a cinnamyl alcohol dehydrogenase involved in lignin biosynthesis in wheat,” Journal of Experimental Botany, vol. 61, no. 10, pp. 2735–2744, 2010. View at Publisher · View at Google Scholar · View at Scopus
  168. Q.-H. Ma, “The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance,” Journal of Experimental Botany, vol. 60, no. 9, pp. 2763–2771, 2009. View at Publisher · View at Google Scholar · View at Scopus
  169. S. Banniza, P. Hashemi, T. D. Warkentin, A. Vandenberg, and A. R. Davis, “The relationships among lodging, stem anatomy, degree of lignification, and resistance to mycosphaerella blight in field pea (Pisum sativum),” Canadian Journal of Botany, vol. 83, no. 8, pp. 954–967, 2005. View at Publisher · View at Google Scholar · View at Scopus
  170. M. Inoue, Z. S. Gao, and H. W. Cai, “QTL analysis of lodging resistance and related traits in Italian ryegrass (Lolium multiflorum Lam.),” Theoretical and Applied Genetics, vol. 109, no. 8, pp. 1576–1585, 2004. View at Publisher · View at Google Scholar · View at Scopus
  171. T. Ookawa and K. Ishihara, “Varietal difference of the cell wall components affecting the bending stress of the culm in relation to the lodging resistance in paddy rice,” Japanese Journal of Crop Science, vol. 62, no. 3, pp. 378–384, 1993. View at Scopus
  172. A. J. Travis, S. D. Murison, D. J. Hirst, K. C. Walker, and A. Chesson, “Comparison of the anatomy and degradability of straw from varieties of wheat and barley that differ in susceptibility to lodging,” Journal of Agricultural Science, vol. 127, no. 1, pp. 1–10, 1996. View at Scopus
  173. J. Wang, J. Zhu, R. Huang, and Y. Yang, “Investigation of cell wall composition related to stem lodging resistance in wheat (Triticum aestivum L. by FTIR spectroscopy),” Plant Signaling & Behavior, vol. 7, pp. 856–863, 2012.
  174. S. E. Sattler, D. L. Funnell-Harris, and J. F. Pedersen, “Brown midrib mutations and their importance to the utilization of maize, sorghum, and pearl millet lignocellulosic tissues,” Plant Science, vol. 178, no. 3, pp. 229–238, 2010. View at Publisher · View at Google Scholar · View at Scopus
  175. A. Ching, K. S. Dhugga, L. Appenzeller et al., “Brittle stalk 2 encodes a putative glycosylphosphatidylinositol-anchored protein that affects mechanical strength of maize tissues by altering the composition and structure of secondary cell walls,” Planta, vol. 224, no. 5, pp. 1174–1184, 2006. View at Publisher · View at Google Scholar · View at Scopus
  176. C. P. Vance, T. K. Kirk, and R. T. Sherwood, “Lignification as a mechanism of disease resistance,” Annual Review of Phytopathology, vol. 18, pp. 259–288, 1980.
  177. D. B. Collinge, “Cell wall appositions: the first line of defence,” Journal of Experimental Botany, vol. 60, no. 2, pp. 351–352, 2009. View at Publisher · View at Google Scholar · View at Scopus
  178. T. Mateille, “Biology of the plant nematode relationship—physiological changes and the defence mechanism of plants,” Nematologica, vol. 40, no. 2, pp. 276–311, 1994. View at Scopus
  179. A. Bhattacharya, P. Sood, and V. Citovsky, “The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection,” Molecular Plant Pathology, vol. 11, no. 5, pp. 705–719, 2010. View at Publisher · View at Google Scholar · View at Scopus
  180. C. J. Baker and E. W. Orlandi, “Active oxygen in plant pathogenesis,” Annual Review of Phytopathology, vol. 33, pp. 299–321, 1995. View at Scopus
  181. R. Angelini, A. Tisi, G. Rea et al., “Involvement of polyamine oxidase in wound healing,” Plant Physiology, vol. 146, no. 1, pp. 162–177, 2008. View at Publisher · View at Google Scholar · View at Scopus
  182. D. Cipollini, Q. Wang, J. G. A. Whitehill, J. R. Powell, P. Bonello, and D. A. Herms, “Distinguishing defensive characteristics in the phloem of ash species resistant and susceptible to emerald ash borer,” Journal of Chemical Ecology, vol. 37, no. 5, pp. 450–459, 2011. View at Publisher · View at Google Scholar · View at Scopus
  183. A. C. Schrotenboer, M. S. Allen, and C. M. Malmstrom, “Modification of native grasses for biofuel production may increase virus susceptibility,” Global Change Biology Bioenergy, vol. 3, pp. 360–374, 2011.
  184. D. L. Funnell-Harris, J. F. Pedersen, and S. E. Sattler, “Alteration in lignin biosynthesis restricts growth of Fusarium spp. in brown midrib sorghum,” Phytopathology, vol. 100, no. 7, pp. 671–681, 2010. View at Publisher · View at Google Scholar · View at Scopus
  185. D. M. Gibson, B. C. King, M. L. Hayes, and G. C. Bergstrom, “Plant pathogens as a source of diverse enzymes for lignocellulose digestion,” Current Opinion in Microbiology, vol. 14, no. 3, pp. 264–270, 2011. View at Publisher · View at Google Scholar · View at Scopus
  186. X. Geng and K. Li, “Degradation of non-phenolic lignin by the white-rot fungus Pycnoporus cinnabarinus,” Applied Microbiology and Biotechnology, vol. 60, no. 3, pp. 342–346, 2002. View at Publisher · View at Google Scholar · View at Scopus
  187. C. Eggert, U. Temp, and K. E. L. Eriksson, “Laccase is essential for lignin degradation by the white-rot fungus Pycnoporus cinnabarinus,” FEBS Letters, vol. 407, no. 1, pp. 89–92, 1997. View at Publisher · View at Google Scholar · View at Scopus
  188. D. Singh and S. Chen, “The white-rot fungus Phanerochaete chrysosporium: conditions for the production of lignin-degrading enzymes,” Applied Microbiology and Biotechnology, vol. 81, no. 3, pp. 399–417, 2008. View at Publisher · View at Google Scholar · View at Scopus
  189. M. J. Dinis, R. M. F. Bezerra, F. Nunes et al., “Modification of wheat straw lignin by solid state fermentation with white-rot fungi,” Bioresource Technology, vol. 100, no. 20, pp. 4829–4835, 2009. View at Publisher · View at Google Scholar · View at Scopus
  190. I. Isroi, R. Millati, S. Syamsiah et al., “Biological pretreatment of lignocelluloses with white-rot fungi and its applications: a review,” BioResources, vol. 6, no. 4, pp. 5224–5259, 2011. View at Scopus
  191. P. Kumar, D. M. Barrett, M. J. Delwiche, and P. Stroeve, “Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production,” Industrial & Engineering Chemistry Research, vol. 48, no. 8, pp. 3713–3729, 2009. View at Publisher · View at Google Scholar · View at Scopus
  192. S. M. Geib, T. R. Filley, P. G. Hatcher et al., “Lignin degradation in wood-feeding insects,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 35, pp. 12932–12937, 2008. View at Publisher · View at Google Scholar · View at Scopus
  193. D. O. Krause, S. E. Denman, R. I. Mackie et al., “Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics,” FEMS Microbiology Reviews, vol. 27, no. 5, pp. 663–693, 2003. View at Publisher · View at Google Scholar · View at Scopus
  194. G. A. Varga and E. S. Kolver, “Microbial and animal limitations to fiber digestion and utilization,” Journal of Nutrition, vol. 127, supplement 5, pp. S819–S823, 1997. View at Scopus
  195. E. Novaes, M. Kirst, V. Chiang, H. Winter-Sederoff, and R. Sederoff, “Lignin and biomass: a negative correlation for wood formation and lignin content in trees,” Plant Physiology, vol. 154, no. 2, pp. 555–561, 2010. View at Publisher · View at Google Scholar · View at Scopus
  196. M. Herrero, C. B. do Valle, N. R. G. Hughes, V. O. de Sabatel, and N. S. Jessop, “Measurements of physical strength and their relationship to the chemical composition of four species of Brachiaria,” Animal Feed Science and Technology, vol. 92, no. 3-4, pp. 149–158, 2001. View at Publisher · View at Google Scholar · View at Scopus
  197. A. Cannas, P. J. Van Soest, and A. N. Pell, “Use of animal and dietary information to predict rumen turnover,” Animal Feed Science and Technology, vol. 106, no. 1–4, pp. 95–117, 2003. View at Publisher · View at Google Scholar · View at Scopus
  198. G. Getachew, M. Blümmel, H. P. S. Makkar, and K. Becker, “In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review,” Animal Feed Science and Technology, vol. 72, no. 3-4, pp. 261–281, 1998. View at Scopus
  199. M. D. Casler and H. J. G. Jung, “Relationships of fibre, lignin, and phenolics to in vitro fibre digestibility in three perennial grasses,” Animal Feed Science and Technology, vol. 125, no. 1-2, pp. 151–161, 2006. View at Publisher · View at Google Scholar · View at Scopus
  200. V. Mechin, O. Argillier, V. Menanteau et al., “Relationship of cell wall composition to in vitro cell wall digestibility of maize inbred line stems,” Journal of the Science of Food and Agriculture, vol. 80, pp. 574–580, 2000.
  201. C. Riboulet, B. Lefèvre, D. Dénoue, and Y. Barrière, “Genetic variation in maize cell wall for lignin content, lignin structure, p-hydroxycinnamic acid content, and digestibility in set of 19 lines at silage harvest maturity,” Maydica, vol. 53, no. 1, pp. 11–19, 2008. View at Scopus
  202. D. I. Gomes, E. Detmann, S. D. C. Valadares Filho et al., “Evaluation of lignin contents in tropical forages using different analytical methods and their correlations with degradation of insoluble fiber,” Animal Feed Science and Technology, vol. 168, no. 3-4, pp. 206–222, 2011. View at Publisher · View at Google Scholar · View at Scopus
  203. H. J. G. Jung and J. F. S. Lamb, “Identification of lucerne stem cell wall traits related to in vitro neutral detergent fibre digestibility,” Animal Feed Science and Technology, vol. 110, no. 1–4, pp. 17–29, 2003. View at Publisher · View at Google Scholar · View at Scopus
  204. J. H. Grabber, D. R. Mertens, H. Kim, C. Funk, F. Lu, and J. Ralph, “Cell wall fermentation kinetics are impacted more by lignin content and ferulate cross-linking than by lignin composition,” Journal of the Science of Food and Agriculture, vol. 89, no. 1, pp. 122–129, 2009. View at Publisher · View at Google Scholar · View at Scopus
  205. J. L. de Boever, B. G. Cottyn, D. L. de Brabander, J. M. Vanacker, and C. V. Boucqué, “Prediction of the feeding value of grass silages by chemical parameters, in vitro digestibility and near-infrared reflectance spectroscopy,” Animal Feed Science and Technology, vol. 60, no. 1-2, pp. 103–115, 1996. View at Scopus
  206. H. G. Jung, D. R. Mertens, and A. J. Payne, “Correlation of acid detergent lignin and klason lignin with digestibility of forage dry matter and neutral detergent fiber,” Journal of Dairy Science, vol. 80, no. 8, pp. 1622–1628, 1997. View at Scopus
  207. M. J. Traxler, D. G. Fox, P. J. van Soest et al., “Predicting forage indigestible NDF from lignin concentration,” Journal of Animal Science, vol. 76, no. 5, pp. 1469–1480, 1998. View at Scopus
  208. I. Karakurt, G. Aydin, and K. Aydiner, “Sources and mitigation of methane emissions by sectors: a critical review,” Renewable Energy, vol. 39, no. 1, pp. 40–48, 2012. View at Publisher · View at Google Scholar · View at Scopus
  209. R. Bodas, N. Prieto, R. Garcia-Gonzalez, S. Andres, F. J. Giraldez, and S. Lopez, “Manipulation of rumen fermentation and methane production with plant secondary metabolites,” Animal Feed Science and Technology, vol. 176, pp. 78–93, 2012.
  210. Y. Wang, T. Marx, J. Lora, L. E. Phillip, and T. A. McAllister, “Effects of purified lignin on in vitro ruminal fermentation and growth performance, carcass traits and fecal shedding of Escherichia coli by feedlot lambs,” Animal Feed Science and Technology, vol. 151, no. 1-2, pp. 21–31, 2009. View at Publisher · View at Google Scholar · View at Scopus
  211. I. K. Hindrichsen, H. R. Wettstein, A. Machmüller et al., “Effects of feed carbohydrates with contrasting properties on rumen fermentation and methane release in vitro,” Canadian Journal of Animal Science, vol. 84, no. 2, pp. 265–276, 2004. View at Scopus
  212. S. Singh, B. P. Kushwaha, S. K. Nag et al., “In vitro methane emission from Indian dry roughages in relation to chemical composition,” Current Science, vol. 101, no. 1, pp. 57–65, 2011. View at Scopus
  213. A. T. W. M. Hendriks and G. Zeeman, “Pretreatments to enhance the digestibility of lignocellulosic biomass,” Bioresource Technology, vol. 100, no. 1, pp. 10–18, 2009. View at Publisher · View at Google Scholar · View at Scopus
  214. T. D. H. Bugg, M. Ahmad, E. M. Hardiman, and R. Rahmanpour, “Pathways for degradation of lignin in bacteria and fungi,” Natural Product Reports, vol. 28, no. 12, pp. 1883–1896, 2011. View at Publisher · View at Google Scholar · View at Scopus
  215. T. van Dinh, J. W. Cone, J. J. P. Baars, A. S. M. Sonnenberg, and W. H. Hendriks, “Fungal strain and incubation period affect chemical composition and nutrient availability of wheat straw for rumen fermentation,” Bioresource Technology, vol. 111, pp. 336–342, 2012. View at Publisher · View at Google Scholar · View at Scopus
  216. B. Shrivastava, S. Thakur, Y. P. Khasa, A. Gupte, A. K. Puniya, and R. C. Kuhad, “White-rot fungal conversion of wheat straw to energy rich cattle feed,” Biodegradation, vol. 22, no. 4, pp. 823–831, 2011. View at Publisher · View at Google Scholar · View at Scopus
  217. M. A. M. Rodrigues, P. Pinto, R. M. F. Bezerra et al., “Effect of enzyme extracts isolated from white-rot fungi on chemical composition and in vitro digestibility of wheat straw,” Animal Feed Science and Technology, vol. 141, no. 3-4, pp. 326–338, 2008. View at Publisher · View at Google Scholar · View at Scopus
  218. C. Sarnklong, J. W. Coneja, W. Pellikaan, and W. H. Hendriks, “Utilization of rice straw and different treatments to improve its feed value for ruminants: a review,” Asian-Australasian Journal of Animal Sciences, vol. 23, no. 5, pp. 680–692, 2010. View at Scopus
  219. M. M. Rahman, M. Lourenço, H. A. Hassim et al., “Improving ruminal degradability of oil palm fronds using white rot fungi,” Animal Feed Science and Technology, vol. 169, no. 3-4, pp. 157–166, 2011. View at Publisher · View at Google Scholar · View at Scopus
  220. D. E. Akin, A. Sethuraman, W. H. Morrison III, S. A. Martin, and K. E. L. Eriksson, “Microbial delignification with white rot fungi improves forage digestibility,” Applied and Environmental Microbiology, vol. 59, no. 12, pp. 4274–4282, 1993. View at Scopus
  221. K. Okano, N. Ohkoshi, A. Nishiyama, T. Usagawa, and M. Kitagawa, “Improving the nutritive value of madake bamboo, Phyllostachys bambusoides, for ruminants by culturing with the white-rot fungus Ceriporiopsis subvermispora,” Animal Feed Science and Technology, vol. 152, no. 3-4, pp. 278–285, 2009. View at Publisher · View at Google Scholar · View at Scopus
  222. J. X. Liu, E. R. Orskov, and X. B. Chen, “Optimization of steam treatment as a method for upgrading rice straw as feeds,” Animal Feed Science and Technology, vol. 76, no. 3-4, pp. 345–357, 1999. View at Publisher · View at Google Scholar · View at Scopus
  223. V. J. H. Sewalt, K. A. Beauchemin, L. M. Rode, S. Acharya, and V. S. Baron, “Lignin impact on fiber degradation. IV. Enzymatic saccharification and in vitro digestibility of alfalfa and grasses following selective solvent delignification,” Bioresource Technology, vol. 61, no. 3, pp. 199–206, 1997. View at Publisher · View at Google Scholar · View at Scopus
  224. Y. Barrière, C. Guillet, D. Goffner, and M. Pichon, “Genetic variation and breeding strategies for improved cell wall digestibility in annual forage crops. A review,” Animal Research, vol. 52, no. 3, pp. 193–228, 2003. View at Scopus
  225. E. M. G. Thorstensson, D. R. Buxton, and J. H. Cherney, “Apparent inhibition to digestion by lignin in normal and brown midrib stems,” Journal of the Science of Food and Agriculture, vol. 59, pp. 183–188, 1992.
  226. M. Goto, J. Matsuoka, T. Sato, H. Ehara, and O. Morita, “Brown midrib mutant maize with reduced levels of phenolic acids ether-linked to the cell walls,” Animal Feed Science and Technology, vol. 48, no. 1-2, pp. 27–38, 1994. View at Scopus
  227. D. N. Ledgerwood, E. J. DePeters, P. H. Robinson, S. J. Taylor, and J. M. Heguy, “Assessment of a brown midrib (BMR) mutant gene on the nutritive value of sudangrass using in vitro and in vivo techniques,” Animal Feed Science and Technology, vol. 150, no. 3-4, pp. 207–222, 2009. View at Publisher · View at Google Scholar · View at Scopus
  228. S. Singh, S. V. S. Prasad, and D. S. Katiyar, “Genetic variability in the fodder yield, chemical composition and disappearance of nutrients in brown midrib and white midrib sorghum genotypes,” Asian-Australasian Journal of Animal Sciences, vol. 16, no. 9, pp. 1303–1308, 2003. View at Scopus
  229. M. Baucher, M. A. Bernard-Vailhé, B. Chabbert et al., “Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility,” Plant Molecular Biology, vol. 39, no. 3, pp. 437–447, 1999. View at Publisher · View at Google Scholar · View at Scopus
  230. G. Getachew, A. M. Ibáñez, W. Pittroff et al., “A comparative study between lignin down regulated alfalfa lines and their respective unmodified controls on the nutritional characteristics of hay,” Animal Feed Science and Technology, vol. 170, no. 3-4, pp. 192–200, 2011. View at Publisher · View at Google Scholar · View at Scopus
  231. X. He, M. B. Hall, M. Gallo-Meagher, and R. L. Smith, “Improvement of forage quality by downregulation of maize O-methyltransferase,” Crop Science, vol. 43, no. 6, pp. 2240–2251, 2003. View at Scopus
  232. H. J. G. Jung, W. T. Ni, C. C. S. Chapple, and K. Meyer, “Impact of lignin composition on cell-wall degradability in an Arabidopsis mutant,” Journal of the Science of Food and Agriculture, vol. 79, pp. 922–928, 1999.
  233. T. Sang, “Toward the domestication of lignocellulosic energy crops: learning from food crop domestication,” Journal of Integrative Plant Biology, vol. 53, no. 2, pp. 96–104, 2011. View at Publisher · View at Google Scholar · View at Scopus
  234. J. S. Yuan, K. H. Tiller, H. Al-Ahmad, N. R. Stewart, and C. N. Stewart Jr., “Plants to power: bioenergy to fuel the future,” Trends in Plant Science, vol. 13, no. 8, pp. 421–429, 2008. View at Publisher · View at Google Scholar · View at Scopus
  235. E. M. Hodgson, S. J. Lister, A. V. Bridgwater, J. Clifton-Brown, and I. S. Donnison, “Genotypic and environmentally derived variation in the cell wall composition of Miscanthus in relation to its use as a biomass feedstock,” Biomass & Bioenergy, vol. 34, no. 5, pp. 652–660, 2010. View at Publisher · View at Google Scholar · View at Scopus
  236. L. Petrus and M. A. Noordermeer, “Biomass to biofuels, a chemical perspective,” Green Chemistry, vol. 8, no. 10, pp. 861–867, 2006. View at Publisher · View at Google Scholar · View at Scopus
  237. J. M. Triolo, L. Pedersen, H. Qu, and S. G. Sommer, “Biochemical methane potential and anaerobic biodegradability of non-herbaceous and herbaceous phytomass in biogas production,” Bioresource Technology, vol. 125, pp. 226–232, 2012.
  238. R. Saidur, E. A. Abdelaziz, A. Demirbas, M. S. Hossain, and S. Mekhilef, “A review on biomass as a fuel for boilers,” Renewable & Sustainable Energy Reviews, vol. 15, no. 5, pp. 2262–2289, 2011. View at Publisher · View at Google Scholar · View at Scopus
  239. C. Gary, J. S. Frossard, and D. Chenevard, “Heat of combustion, degree of reduction and carbon content. 3. Interrelated methods of estimating the construction cost of plant-tissues,” Agronomie, vol. 15, no. 1, pp. 59–69, 1995. View at Scopus
  240. V. Mendu, T. Shearin, J. E. Campbell Jr. et al., “Global bioenergy potential from high-lignin agricultural residue,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 10, pp. 4014–4019, 2012. View at Publisher · View at Google Scholar · View at Scopus
  241. W. J. Orts, K. M. Holtman, and J. N. Seiber, “Agricultural chemistry and bioenergy,” Journal of Agricultural and Food Chemistry, vol. 56, no. 11, pp. 3892–3899, 2008. View at Publisher · View at Google Scholar · View at Scopus
  242. Y. Lu, X.-Y. Wei, J.-P. Cao et al., “Characterization of a bio-oil from pyrolysis of rice husk by detailed compositional analysis and structural investigation of lignin,” Bioresource Technology, vol. 116, pp. 114–119, 2012.
  243. F. Melligan, K. Dussan, R. Auccaise et al., “Characterisation of the products from pyrolysis of residues after acid hydrolysis of Miscanthus,” Bioresource Technology, vol. 108, pp. 258–263, 2012. View at Publisher · View at Google Scholar · View at Scopus
  244. P. Rutkowski, “Pyrolysis of cellulose, xylan and lignin with the K2CO3 and ZnCl2 addition for bio-oil production,” Fuel Processing Technology, vol. 92, no. 3, pp. 517–522, 2011. View at Publisher · View at Google Scholar · View at Scopus
  245. A. A. Boateng, P. J. Weimer, H. G. Jung, and J. F. S. Lamb, “Response of thermochemical and biochemical conversion processes to lignin concentration in alfalfa stems,” Energy and Fuels, vol. 22, no. 4, pp. 2810–2815, 2008. View at Publisher · View at Google Scholar · View at Scopus
  246. R. Fahmi, A. V. Bridgwater, I. Donnison, N. Yates, and J. M. Jones, “The effect of lignin and inorganic species in biomass on pyrolysis oil yields, quality and stability,” Fuel, vol. 87, no. 7, pp. 1230–1240, 2008. View at Publisher · View at Google Scholar · View at Scopus
  247. E. M. Hodgson, D. J. Nowakowski, I. Shield et al., “Variation in Miscanthus chemical composition and implications for conversion by pyrolysis and thermo-chemical bio-refining for fuels and chemicals,” Bioresource Technology, vol. 102, no. 3, pp. 3411–3418, 2011. View at Publisher · View at Google Scholar · View at Scopus
  248. Y. Zeng, X. Yang, H. Yu, X. Zhang, and F. Ma, “Comparative studies on thermochemical characterization of corn stover pretreated by white-rot and brown-rot fungi,” Journal of Agricultural and Food Chemistry, vol. 59, no. 18, pp. 9965–9971, 2011. View at Publisher · View at Google Scholar · View at Scopus
  249. E. G. Pereira, J. N. da Silva, J. L. de Oliveira, and C. S. Machado, “Sustainable energy: a review of gasification technologies,” Renewable & Sustainable Energy Reviews, vol. 16, pp. 4753–4762, 2012.
  250. V. Subramani and S. K. Gangwal, “A review of recent literature to search for an efficient catalytic process for the conversion of syngas to ethanol,” Energy and Fuels, vol. 22, no. 2, pp. 814–839, 2008. View at Publisher · View at Google Scholar · View at Scopus
  251. H. P. Yang, R. Yan, H. P. Chen, D. H. Lee, and C. G. Zheng, “Characteristics of hemicellulose, cellulose and lignin pyrolysis,” Fuel, vol. 86, no. 12-13, pp. 1781–1788, 2007. View at Publisher · View at Google Scholar · View at Scopus
  252. A. G. Barneto, J. A. Carmona, A. Gálvez, and J. A. Conesa, “Effects of the composting and the heating rate on biomass gasification,” Energy and Fuels, vol. 23, no. 2, pp. 951–957, 2009. View at Publisher · View at Google Scholar · View at Scopus
  253. A. G. Barneto, J. A. Carmona, J. A. C. Ferrer, and M. J. D. Blanco, “Kinetic study on the thermal degradation of a biomass and its compost: composting effect on hydrogen production,” Fuel, vol. 89, no. 2, pp. 462–473, 2010. View at Publisher · View at Google Scholar · View at Scopus
  254. N. Sarkar, S. K. Ghosh, S. Bannerjee, and K. Aikat, “Bioethanol production from agricultural wastes: an overview,” Renewable Energy, vol. 37, no. 1, pp. 19–27, 2012. View at Publisher · View at Google Scholar · View at Scopus
  255. J. Lindedam, S. B. Andersen, J. DeMartini et al., “Cultivar variation and selection potential relevant to the production of cellulosic ethanol from wheat straw,” Biomass & Bioenergy, vol. 37, pp. 221–228, 2012. View at Publisher · View at Google Scholar · View at Scopus
  256. A. V. Lygin, J. Upton, F. G. Dohleman et al., “Composition of cell wall phenolics and polysaccharides of the potential bioenergy crop—Miscanthus,” Global Change Biology Bioenergy, vol. 3, pp. 333–345, 2011.
  257. A. J. Lorenz, R. P. Anex, A. Isci, J. G. Coors, N. de Leon, and P. J. Weimer, “Forage quality and composition measurements as predictors of ethanol yield from maize (Zea mays L.) stover,” Biotechnology for Biofuels, vol. 2, article 5, 2009. View at Publisher · View at Google Scholar · View at Scopus
  258. W. F. Anderson, B. S. Dien, H.-J. G. Jung, K. P. Vogel, and P. J. Weimer, “Effects of forage quality and cell wall constituents of Bermuda grass on biochemical conversion to ethanol,” Bioenergy Research, vol. 3, no. 3, pp. 225–237, 2010. View at Publisher · View at Google Scholar · View at Scopus
  259. F. Chen and R. A. Dixon, “Lignin modification improves fermentable sugar yields for biofuel production,” Nature Biotechnology, vol. 25, no. 7, pp. 759–761, 2007. View at Publisher · View at Google Scholar · View at Scopus
  260. C. Fu, J. R. Mielenz, X. Xiao et al., “Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass,” Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 9, pp. 3803–3808, 2011. View at Publisher · View at Google Scholar · View at Scopus
  261. J. M. Kusiima and S. E. Powers, “Monetary value of the environmental and health externalities associated with production of ethanol from biomass feedstocks,” Energy Policy, vol. 38, no. 6, pp. 2785–2796, 2010. View at Publisher · View at Google Scholar · View at Scopus
  262. K. Minu, K. K. Jiby, and V. V. N. Kishore, “Isolation and purification of lignin and silica from the black liquor generated during the production of bioethanol from rice straw,” Biomass & Bioenergy, vol. 39, pp. 210–217, 2012. View at Publisher · View at Google Scholar · View at Scopus
  263. F. Monlau, C. Sambusiti, A. Barakat et al., “Predictive models of biohydrogen and biomethane production based on the compositional and structural features of lignocellulosic materials,” Environmental Science & Technology, vol. 46, pp. 12217–12225, 2012.
  264. E. A. Tsavkelova and A. I. Netrusov, “Biogas production from cellulose-containing substrates: a review,” Applied Biochemistry and Microbiology, vol. 48, pp. 421–433, 2012.
  265. E. Klimiuk, T. Pokój, W. Budzyński, and B. Dubis, “Theoretical and observed biogas production from plant biomass of different fibre contents,” Bioresource Technology, vol. 101, no. 24, pp. 9527–9535, 2010. View at Publisher · View at Google Scholar · View at Scopus
  266. C. Grieder, B. S. Dhillon, W. Schipprack, and A. E. Melchinger, “Breeding maize as biogas substrate in Central Europe: II. Quantitative-genetic parameters for inbred lines and correlations with testcross performance,” Theoretical and Applied Genetics, vol. 124, pp. 981–988, 2012. View at Publisher · View at Google Scholar · View at Scopus
  267. J. M. Triolo, S. G. Sommer, H. B. Møller, M. R. Weisbjerg, and X. Y. Jiang, “A new algorithm to characterize biodegradability of biomass during anaerobic digestion: Influence of lignin concentration on methane production potential,” Bioresource Technology, vol. 102, no. 20, pp. 9395–9402, 2011. View at Publisher · View at Google Scholar · View at Scopus
  268. D. P. Komilis and R. K. Ham, “The effect of lignin and sugars to the aerobic decomposition of solid wastes,” Waste Management, vol. 23, no. 5, pp. 419–423, 2003. View at Publisher · View at Google Scholar · View at Scopus
  269. P. Buffiere, D. Loisel, N. Bernet, and J. P. Delgenes, “Towards new indicators for the prediction of solid waste anaerobic digestion properties,” Water Science and Technology, vol. 53, no. 8, pp. 233–241, 2006. View at Publisher · View at Google Scholar · View at Scopus
  270. M. J. Taherzadeh and K. Karimi, “Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review,” International Journal of Molecular Sciences, vol. 9, no. 9, pp. 1621–1651, 2008. View at Publisher · View at Google Scholar · View at Scopus
  271. M. Carlsson, A. Lagerkvist, and F. Morgan-Sagastume, “The effects of substrate pre-treatment on anaerobic digestion systems: a review,” Waste Management, vol. 32, pp. 1634–1650, 2012.
  272. T. Mackulak, J. Prousek, L. Svorc, and M. Drtil, “Increase of biogas production from pretreated hay and leaves using wood-rotting fungi,” Chemical Papers, vol. 66, pp. 649–653, 2012.
  273. K. Ziemiński, I. Romanowska, and M. Kowalska, “Enzymatic pretreatment of lignocellulosic wastes to improve biogas production,” Waste Management, vol. 32, no. 6, pp. 1131–1137, 2012. View at Publisher · View at Google Scholar · View at Scopus
  274. Z. Song, G. Yang, Y. Guo, and T. Zhang, “Comparison of two chemical pretreatments of rice straw for biogas production by anaerobic digestion,” BioResources, vol. 7, pp. 3223–3236, 2012.
  275. F. Monlau, A. Barakat, J. P. Steyer, and H. Carrere, “Comparison of seven types of thermo-chemical pretreatments on the structural features and anaerobic digestion of sunflower stalks,” Bioresource Technology, vol. 120, pp. 241–247, 2012.
  276. R. Vanholme, K. Morreel, C. Darrah et al., “Metabolic engineering of novel lignin in biomass crops,” New Phytologist, vol. 196, pp. 978–1000, 2012.
  277. J.-K. Weng, X. Li, N. D. Bonawitz, and C. Chapple, “Emerging strategies of lignin engineering and degradation for cellulosic biofuel production,” Current Opinion in Biotechnology, vol. 19, no. 2, pp. 166–172, 2008. View at Publisher · View at Google Scholar · View at Scopus
  278. M. Tuomela, M. Vikman, A. Hatakka, and M. Itävaara, “Biodegradation of lignin in a compost environment: a review,” Bioresource Technology, vol. 72, no. 2, pp. 169–183, 2000. View at Publisher · View at Google Scholar · View at Scopus
  279. W. Zech, N. Senesi, G. Guggenberger et al., “Factors controlling humification and mineralization of soil organic matter in the tropics,” Geoderma, vol. 79, no. 1–4, pp. 117–161, 1997. View at Publisher · View at Google Scholar · View at Scopus
  280. B. Marschner, S. Brodowski, A. Dreves et al., “How relevant is recalcitrance for the stabilization of organic matter in soils?” Journal of Plant Nutrition and Soil Science, vol. 171, no. 1, pp. 91–110, 2008. View at Publisher · View at Google Scholar · View at Scopus
  281. W. Amelung, K. W. Flach, and W. Zech, “Climatic effects on soil organic matter composition in the Great Plains,” Soil Science Society of America Journal, vol. 61, no. 1, pp. 115–123, 1997. View at Scopus
  282. R. Kiem and I. Kögel-Knabner, “Contribution of lignin and polysaccharides to the refractory carbon pool in C-depleted arable soils,” Soil Biology & Biochemistry, vol. 35, no. 1, pp. 101–118, 2003. View at Publisher · View at Google Scholar · View at Scopus
  283. G. Gleixner, N. Poirier, R. Bol, and J. Balesdent, “Molecular dynamics of organic matter in a cultivated soil,” Organic Geochemistry, vol. 33, no. 3, pp. 357–366, 2002. View at Publisher · View at Google Scholar · View at Scopus
  284. E. M. Carrington, P. J. Hernes, R. Y. Dyda, A. F. Plante, and J. Six, “Biochemical changes across a carbon saturation gradient: lignin, cutin, and suberin decomposition and stabilization in fractionated carbon pools,” Soil Biology & Biochemistry, vol. 47, pp. 179–190, 2012. View at Publisher · View at Google Scholar · View at Scopus
  285. W. Amelung, K.-W. Flach, and W. Zech, “Lignin in particle-size fractions of native grassland soils as influenced by climate,” Soil Science Society of America Journal, vol. 63, no. 5, pp. 1222–1228, 1999. View at Scopus
  286. M. Thevenot, M.-F. Dignac, and C. Rumpel, “Fate of lignins in soils: a review,” Soil Biology & Biochemistry, vol. 42, no. 8, pp. 1200–1211, 2010. View at Publisher · View at Google Scholar · View at Scopus
  287. T. Osono, “Ecology of ligninolytic fungi associated with leaf litter decomposition,” Ecological Research, vol. 22, no. 6, pp. 955–974, 2007. View at Publisher · View at Google Scholar · View at Scopus
  288. R. L. Sinsabaugh, “Phenol oxidase, peroxidase and organic matter dynamics of soil,” Soil Biology & Biochemistry, vol. 42, no. 3, pp. 391–404, 2010. View at Publisher · View at Google Scholar · View at Scopus
  289. A. T. Austin and C. L. Ballaré, “Dual role of lignin in plant litter decomposition in terrestrial ecosystems,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 10, pp. 4618–4622, 2010. View at Publisher · View at Google Scholar · View at Scopus
  290. M. J. Simpson and A. J. Simpson, “The chemical ecology of soil organic matter molecular constituents,” Journal of Chemical Ecology, vol. 38, no. 6, pp. 768–784, 2012. View at Publisher · View at Google Scholar · View at Scopus
  291. E. C. Adair, W. J. Parton, S. J. del Grosso et al., “Simple three-pool model accurately describes patterns of long-term litter decomposition in diverse climates,” Global Change Biology, vol. 14, no. 11, pp. 2636–2660, 2008. View at Publisher · View at Google Scholar · View at Scopus
  292. B. R. Taylor, D. Parkinson, and W. F. J. Parsons, “Nitrogen and lignin content as predictors of litter decay rates—a microcosm test,” Ecology, vol. 70, no. 1, pp. 97–104, 1989. View at Scopus
  293. M. Becker, J. K. Ladha, and J. C. G. Ottow, “Nitrogen losses and lowland rice yield as affected by residue nitrogen release,” Soil Science Society of America Journal, vol. 58, no. 6, pp. 1660–1665, 1994. View at Scopus
  294. M. Becker, J. K. Ladha, I. C. Simpson, and J. C. G. Ottow, “Parameters affecting residue nitrogen mineralization in flooded soils,” Soil Science Society of America Journal, vol. 58, no. 6, pp. 1666–1671, 1994. View at Scopus
  295. J. G. Cobo, E. Barrios, D. C. L. Kass, and R. J. Thomas, “Decomposition and nutrient release by green manures in a tropical hillside agroecosystem,” Plant and Soil, vol. 240, no. 2, pp. 331–342, 2002. View at Publisher · View at Google Scholar · View at Scopus
  296. J. H. C. Cornelissen, H. M. Quested, D. Gwynn-Jones et al., “Leaf digestibility and litter decomposability are related in a wide range of subarctic plant species and types,” Functional Ecology, vol. 18, no. 6, pp. 779–786, 2004. View at Publisher · View at Google Scholar · View at Scopus
  297. C. A. Palm and P. A. Sanchez, “Nitrogen release from the leaves of some tropical legumes as affected by their lignin and polyphenolic contents,” Soil Biology & Biochemistry, vol. 23, no. 1, pp. 83–88, 1991. View at Scopus
  298. K. Kalbitz, K. Kaiser, J. Bargholz, and P. Dardenne, “Lignin degradation controls the production of dissolved organic matter in decomposing foliar litter,” European Journal of Soil Science, vol. 57, no. 4, pp. 504–516, 2006. View at Publisher · View at Google Scholar · View at Scopus
  299. G. E. Machinet, I. Bertrand, Y. Barrière, B. Chabbert, and S. Recous, “Impact of plant cell wall network on biodegradation in soil: role of lignin composition and phenolic acids in roots from 16 maize genotypes,” Soil Biology & Biochemistry, vol. 43, no. 7, pp. 1544–1552, 2011. View at Publisher · View at Google Scholar · View at Scopus
  300. F. Liebner, G. P. Four, J. M. de la Rosa Arranz, A. Hilseher, T. Rosenau, and H. Knicker, “Ammonoxidised lignins as slow nitrogen-releasing soil amendments and CO2-binding matrix,” Angewandte Chemie, vol. 50, no. 37, pp. A34–A39, 2011. View at Scopus
  301. N. H. Batjes, “Total carbon and nitrogen in the soils of the world,” European Journal of Soil Science, vol. 47, no. 2, pp. 151–163, 1996. View at Scopus
  302. H. Blanco-Canqui and R. Lal, “Mechanisms of carbon sequestration in soil aggregates,” Critical Reviews in Plant Sciences, vol. 23, no. 6, pp. 481–504, 2004. View at Publisher · View at Google Scholar · View at Scopus
  303. F. A. Dijkstra, S. E. Hobbie, J. M. H. Knops, and P. B. Reich, “Nitrogen deposition and plant species interact to influence soil carbon stabilization,” Ecology Letters, vol. 7, no. 12, pp. 1192–1198, 2004. View at Publisher · View at Google Scholar · View at Scopus
  304. B. Mandal, B. Majumder, T. K. Adhya et al., “Potential of double-cropped rice ecology to conserve organic carbon under subtropical climate,” Global Change Biology, vol. 14, no. 9, pp. 2139–2151, 2008. View at Publisher · View at Google Scholar · View at Scopus
  305. E. A. Davidson and I. A. Janssens, “Temperature sensitivity of soil carbon decomposition and feedbacks to climate change,” Nature, vol. 440, no. 7081, pp. 165–173, 2006. View at Publisher · View at Google Scholar · View at Scopus
  306. X. Feng, A. J. Simpson, K. P. Wilson, D. D. Williams, and M. J. Simpson, “Increased cuticular carbon sequestration and lignin oxidation in response to soil warming,” Nature Geoscience, vol. 1, no. 12, pp. 836–839, 2008. View at Publisher · View at Google Scholar · View at Scopus
  307. Y. Barriere and O. Argillier, “Brown-midrib genes of maize—a review,” Agronomie, vol. 13, no. 10, pp. 865–876, 1993. View at Scopus
  308. F. Vignols, J. Rigau, M. A. Torres, M. Capellades, and P. Puigdomenech, “The brown midrib3 (bm3) mutation in maize occurs in the gene encoding caffeic acid O-methyltransferase,” Plant Cell, vol. 7, no. 4, pp. 407–416, 1995. View at Publisher · View at Google Scholar · View at Scopus
  309. Y. Chen, H. Liu, F. Ali et al., “Genetic and physical fine mapping of the novel brown midrib gene bm6 in maize (Zea mays L.) to a 180 kb region on chromosome 2,” Theoretical and Applied Genetics, vol. 125, pp. 1223–1235, 2012.
  310. K. E. Tjardes, D. D. Buskirk, M. S. Allen, N. K. Ames, L. D. Bourquin, and S. R. Rust, “Brown midrib-3 corn silage improves digestion but not performance of growing beef steers,” Journal of Animal Science, vol. 78, no. 11, pp. 2957–2965, 2000. View at Scopus
  311. A. L. Oliver, R. J. Grant, J. F. Pedersen, and J. O'Rear, “Comparison of brown midrib-6 and -18 forage sorghum with conventional sorghum and corn silage in diets of lactating dairy cows,” Journal of Dairy Science, vol. 87, no. 3, pp. 637–644, 2004. View at Scopus
  312. B. S. Dien, G. Sarath, J. F. Pedersen et al., “Improved sugar conversion and ethanol yield for forage sorghum (Sorghum bicolor L. Moench) lines with reduced lignin contents,” Bioenergy Research, vol. 2, no. 3, pp. 153–164, 2009. View at Publisher · View at Google Scholar · View at Scopus
  313. A. J. Lorenz, J. G. Coors, N. de Leon et al., “Characterization, genetic variation, and combining ability of maize traits relevant to the production of cellulosic ethanol,” Crop Science, vol. 49, no. 1, pp. 85–98, 2009. View at Publisher · View at Google Scholar · View at Scopus
  314. A. Saballos, W. Vermerris, L. Rivera, and G. Ejeta, “Allelic association, chemical characterization and saccharification properties of brown midrib mutants of sorghum (Sorghum bicolor (L.) Moench),” Bioenergy Research, vol. 1, pp. 193–204, 2008.
  315. P. M. White, C. W. Rice, J. A. Baldock, and M. R. Tuinstra, “Soil biological properties following additions of bmr mutant grain sorghum,” Soil Biology & Biochemistry, vol. 39, no. 7, pp. 1518–1532, 2007. View at Publisher · View at Google Scholar · View at Scopus
  316. Y. Barrière, V. Méchin, F. Lafarguette et al., “Toward the discovery of maize cell wall genes involved in silage quality and capacity to biofuel production,” Maydica, vol. 54, no. 2-3, pp. 161–198, 2009. View at Scopus
  317. Z. Dimov, E. Suprianto, F. Hermann, and C. Möllers, “Genetic variation for seed hull and fibre content in a collection of European winter oilseed rape material (Brassica napus L.) and development of NIRS calibrations,” Plant Breeding, vol. 131, pp. 361–368, 2012. View at Publisher · View at Google Scholar · View at Scopus
  318. C. Shi, A. Uzarowska, M. Ouzunova, M. Landbeck, G. Wenzel, and T. Lübberstedt, “Identification of candidate genes associated with cell wall digestibility and eQTL (expression quantitative trait loci) analysis in a Flint × Flint maize recombinant inbred line population,” BMC Genomics, vol. 8, article 22, 2007. View at Publisher · View at Google Scholar · View at Scopus
  319. P. L. Morrell, E. S. Buckler, and J. Ross-Ibarra, “Crop genomics: advances and applications,” Nature Reviews Genetics, vol. 13, no. 2, pp. 85–96, 2012. View at Publisher · View at Google Scholar · View at Scopus
  320. C. Riedelsheimer, J. Lisec, A. Czedik-Eysenberg et al., “Genome-wide association mapping of leaf metabolic profiles for dissecting complex traits in maize,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, pp. 8872–8877, 2012.
  321. H. Hisano, R. Nandakumar, and Z.-Y. Wang, “Genetic modification of lignin biosynthesis for improved biofuel production,” In Vitro Cellular & Developmental Biology, vol. 45, no. 3, pp. 306–313, 2009. View at Publisher · View at Google Scholar · View at Scopus
  322. S. Fornale, M. Capellades, A. Encina et al., “Altered lignin biosynthesis improves cellulosic bioethanol production in transgenic maize plants down-regulated for cinnamyl alcohol dehydrogenase,” Molecular Plant, vol. 5, pp. 817–830, 2012.
  323. J. Piquemal, S. Chamayou, I. Nadaud et al., “Down-regulation of caffeic acid O-methyltransferase in maize revisited using a transgenic approach,” Plant Physiology, vol. 130, no. 4, pp. 1675–1685, 2002. View at Publisher · View at Google Scholar · View at Scopus
  324. D. G. Guo, F. Chen, J. Wheeler et al., “Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferases,” Transgenic Research, vol. 10, no. 5, pp. 457–464, 2001. View at Publisher · View at Google Scholar · View at Scopus
  325. J. H. Jung, W. M. Fouad, W. Vermerris, M. Gallo, and F. Altpeter, “RNAi suppression of lignin biosynthesis in sugarcane reduces recalcitrance for biofuel production from lignocellulosic biomass,” Plant Biotechnology Journal, vol. 10, pp. 1067–1076, 2012.
  326. M. S. S. Reddy, F. Chen, G. Shadle, L. Jackson, H. Aljoe, and R. A. Dixon, “Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.),” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 46, pp. 16573–16578, 2005. View at Publisher · View at Google Scholar · View at Scopus
  327. F. Fornal, X. Shi, C. Chai et al., “ZmMYB31 directly represses maize lignin genes and redirects the phenylpropanoid metabolic flux,” Plant Journal, vol. 64, no. 4, pp. 633–644, 2010. View at Publisher · View at Google Scholar · View at Scopus
  328. S. Rastogi and U. N. Dwivedi, “Manipulation of lignin in plants with special reference to O-methyltransferase,” Plant Science, vol. 174, no. 3, pp. 264–277, 2008. View at Publisher · View at Google Scholar · View at Scopus
  329. C. E. L. Fonseca, D. R. Viands, J. L. Hansen, and A. N. Pell, “Associations among forage quality traits, vigor, and disease resistance in alfalfa,” Crop Science, vol. 39, no. 5, pp. 1271–1276, 1999. View at Scopus
  330. Y. Chen, I. Zein, E. A. Brenner et al., “Polymorphisms in monolignol biosynthetic genes are associated with biomass yield and agronomic traits in European maize (Zea mays L.),” BMC Plant Biology, vol. 10, article 12, 2010. View at Publisher · View at Google Scholar · View at Scopus
  331. J. H. Grabber, D. Ress, and J. Ralph, “identifying new lignin bioengineering targets: impact of epicatechin, quercetin glycoside, and gallate derivatives on the lignification and fermentation of maize cell walls,” Journal of Agricultural and Food Chemistry, vol. 60, pp. 5152–5160, 2012.
  332. J. H. Grabber, P. F. Schatz, H. Kim, F. Lu, and J. Ralph, “Identifying new lignin bioengineering targets: 1. Monolignol-substitute impacts on lignin formation and cell wall fermentability,” BMC Plant Biology, vol. 10, article 114, 2010. View at Publisher · View at Google Scholar · View at Scopus