Analysis of the Arabidopsis Mitogen-Activated Protein Kinase Families: Organ Specificity and Transcriptional Regulation upon Water Stresses

Moustafa, Khaled; Lefebvre-De Vos, Delphine; Leprince, Anne-Sophie; Savouré, Arnould; Laurière, Christiane

doi:https://doi.org/10.3814/2008/143656

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Volume 2008 | Article ID 143656 | https://doi.org/10.3814/2008/143656

Analysis of the Arabidopsis Mitogen-Activated Protein Kinase Families: Organ Specificity and Transcriptional Regulation upon Water Stresses

Khaled Moustafa,¹Delphine Lefebvre-De Vos,²Anne-Sophie Leprince,²Arnould Savouré,²and Christiane Laurière¹

Received02 Jul 2008

Accepted06 Oct 2008

Published11 Nov 2008

Abstract

The Arabidopsis genome contains 20 MPK, 10 MKK, 60 MAP3K, and 10 MAP4K genes, but the functions of the corresponding proteins are still poorly characterized. To research possible functional MAP kinase modules involved in water stress signalling, original macroarrays were developed for their sensitivity and specificity. The expression patterns of the MPK and MKK gene families, as well as selected key members of the MAP3K and MAP4K families, were studied in relation to organ specificity and to various water stresses. Several kinases of the scarcely studied MPK group containing a TDY activation motif were induced by water stresses. There are four different MPKs (MPK9, MPK11, MPK17, and MPK18), among which three contain the TDY activation motif, and MKK9 and MAP3K6 are induced at the transcriptional level by dehydration, salinity, and hyperosmolarity treatments. They probably constitute MAP kinase key elements involved in water stress signalling.

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Copyright © 2008 Khaled Moustafa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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