Cardiovascular Psychiatry and Neurology

Research Article

S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation

Table 1

Cholinergic neurons after glucose-oxygen deprivation and neuroprotection with S100b.


Treatment		neurons	p1	p2

Day 0		(14)	—
+1d	GD	(5)	ns
	OD	(6)	ns
	OGD	(6)	ns	—
	OGD + S100b (50 g/mL)	(3)		ns
+2d	GD	(5)	ns
	OD	(8)	ns
	OGD	(6)	ns	—
	OGD + S100b (50 g/mL)	(6)		ns
+3d	(-)	(18)	—
	S100b (50 g/mL)	(19)	ns
	GD	(13)	ns
	OD	(12)	ns
	OGD	(28)	***	—
	OGD + S100b (50 g/mL)	(26)	ns	***
	OGD + S100b (1 g/mL)	(14)	**	ns
	OGD + S100b (0.1 g/mL)	(14)	*	ns
	OGD + FGF-2 (2 ng/mL)	(12)		ns
	OGD + NGF (10 ng/mL)	(18)	*	ns

Brain slices of the nBM were cultured for 2 weeks with 10 ng/mL nerve growth factor (NGF), then for 3 days without NGF and then treated under oxygen-glucose deprivation (OGD) with or without S100b or NGF or fibroblast growth factor-2 (FGF-2) for 1–3 days. Values are given as mean ± SEM ( in parentheses). Statistical analysis was performed by one-way ANOVA with a subsequent Fisher PLSD posthoc test and compared against the untreated control (p1) or against OGD (p2) (*; **; ***; ns not significant). GD, glucose deprivation; OD, oxygen deprivation; OGD, oxygen and glucose deprivation.