Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Dosage</td><td align="center">0.65 nmol<br/>siNM_011661_001</td><td align="left">1.3 nmol<br/>siNM_011661_001</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">Interference<br/> efficiency<math id="M45" xmlns="http://www.w3.org/1998/Math/MathML"><mrow><msup><mrow></mrow><mrow><mi>*</mi></mrow></msup></mrow></math></td><td align="center">25.25% <math id="M46" xmlns="http://www.w3.org/1998/Math/MathML"><mrow><mo>±</mo></mrow></math> 0.0355</td><td align="left">59.85% <math id="M47" xmlns="http://www.w3.org/1998/Math/MathML"><mrow><mo>±</mo></mrow></math> 0.0276</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><math id="M48" xmlns="http://www.w3.org/1998/Math/MathML"><mrow><msup><mrow></mrow><mrow><mi>*</mi></mrow></msup></mrow></math>Twenty-four hours after injection, qRT-PCR showed that the tyrosinase levels in the retinas of mice treated with 0.65 and 1.3 nmol siNM_011661_001 siRNA were reduced by 25.25% <math id="M49" xmlns="http://www.w3.org/1998/Math/MathML"><mrow><mo>±</mo></mrow></math> 0.0355 and 59.85% <math id="M50" xmlns="http://www.w3.org/1998/Math/MathML"><mrow><mo>±</mo></mrow></math> 0.0276 (<i>P  </i><math id="M51" xmlns="http://www.w3.org/1998/Math/MathML"><mo>&lt;</mo><mi>  </mi></math>.05), respectively, compared with retinas of eyes injected with PBS.<br/></td></tr></table>

The tyrosinase mRNA levels in mice receiving different dosages of siNM_011661_001.

Molecular Biology International

tab1

Table 1

Table 1: Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo 