B-factors for X-ray structures of TRTK12-Ca²⁺-S100B (2.0 Å) and Ca²⁺-S100B (1.5 Å) from the PI’s laboratory. (a) B-factors for backbone atoms for each subunit of Ca²⁺-S100B (blue, green) and for TRTK-Ca²⁺-S100B (red). (b) B-factors for sidechains with symbols as in (a). (c) Also shown is a ribbon diagram of the TRTK12-Ca²⁺-S100B structure with residues colored red in EF2 (residues 61–72), which display lower B-factors in the TRTK12-bound state (in panels (a) and (b)). These data are all published in Charpentier et al., 2010 [139].