Research Article
Assigning Significance in Label-Free Quantitative Proteomics to Include Single-Peptide-Hit Proteins with Low Replicates
Table 1
The five unlabeled protein samples from culture S or R, the five protein sample preparation replicates of the labeled proteins from culture C, and the corresponding 20 quantitation categories (see Methods.
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, , and were the unlabeled protein samples from the S culture triplicates. was the pool of , , and . was the pool of the unlabeled proteins from the culture R triplicates. The subscripts “1” and “2” represent the LC/MS duplicates. Only the 3rd gel fraction for samples , , and along with the labeled control in them was analyzed by LC/MS. All of the five gel fractions were analyzed with LC/MS for samples and along with the labeled control in them. |