Research Article

Assigning Significance in Label-Free Quantitative Proteomics to Include Single-Peptide-Hit Proteins with Low Replicates

Table 3

Numbers of differentially regulated proteins selected with PLGEM-STN alone or in combination with MPSP .

PLGEM-STN confidence level FP, P, and FDRPLGEM-STNPLGEM-STN-MPSP
Permuted sample pairings Average
IIIIIIIV

0.01FP (c / c )316822464213
P ( / )141155134148145101
FDR0.220.440.160.310.290.13

0.002FP (c / c )6153982
P ( / )475046514944
FDR0.130.300.070.180.160.05

alse positives (FP) were selected from sample pair c /c . Positives (P) were selected from sample pair / . False discovery rate (FDR) was FP/P. The four permuted sample pairings (I–IV) were generated from the four LC/MS injections for a sample pair. See text.