Examinations of tRNA Range of Motion Using Simulations of Cryo-EM Microscopy and X-Ray Data
Figure 1
tRNA Conformations. Both kinked structures for tRNAPhe (PDB code: 1EHZ) and the hybrid state for cryo-EM densities of tRNA are shown. The different orientations of tRNA depict the unique differences caused from kinking or twisting (A/T and P/E states, resp.). The arrows depict the transition from A/T state to A-site (A/A state) to P-site and P/E hybrid state. (a) Secondary structure for Phe-tRNAPhe (1EHZ) [33]. (b) Structure of kinked A/T-tRNA, as it enters the ribosome (pre-accommodation) [13]. (c) A-site tRNA after accommodation occurs (post accommodation). (d) Hybrid P/E-tRNA following peptidyl transfer. (e) Comparison of A-site tRNA and kinked A/T-tRNA from modeling with cryo-EM density. (f) Position of tRNA in A-site indicated in cryo-EM density. (g) Conformation sampled from MD demonstrating an experimentally unidentified twisted conformation for tRNAPhe.
