In Vitro and Ex Vivo Evaluation of Smart Infra-Red Fluorescent Caspase-3 Probes for Molecular Imaging of Cardiovascular Apoptosis
Figure 1
(a) Typical flow cytometry signal obtained in HME cells. Cells were either untreated (control) or incubated with staurosporine 1 μM for 6 h, and percentage of cell death, derived from the flow cytometry analysis, obtained in control or staurosporine-treated HME cells. Data are mean ± SEM (). (b) Caspase-3 activity in HME cells. Cells were either untreated (control) or treated with staurosporine in the absence or the presence of the caspase-3 inhibitor Z-Asp(OMe)-Gln-Met-Asp(OMe)-CH2F (100 μM). R.L.U.: relative luminescent units. Data are mean ± SEM (). ** versus controls; † versus staurosporine alone.
