Research Article
Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis
Table 1
Primers used in this work.
| | Primer | Sequence (5′–3′) | Position* | Amplicon | Gene |
| | BamHI-1-738-F | TACCAGATCGGTTGATGTGC | 1227–1246 | 738 | UL56 | | BamHI-1-738-R | AACAGGAGCGTCTCCGAGTA | 1945–1964 | | BamHI-3-256-F | GTAGGCCGCGTAGAACTGC | 37671–37689 | 256 | UL36 | | BamHI-3-256-R | GCGCATCGAGAGCAAGTA | 37909–37926 | | BamHI-4-357-F | GAGCAGCATGATCGTCGTC | 55372–55390 | 357 | UL44, UL26, UL26.5 | | BamHI-4-357-R | TTCGTCTCGCAGATGATGTC | 55709–55728 | | BamHI-6/7-637-F | GAGTCCAAGGACATGGAGGA | 62380–62399 | 637 | UL22 | | BamHI-6/7-637-R | GTCTCACACACAACCGGGTA | 62997–63016 | | BamHI-16/17-799-F | GAGATGCACCTGATCGACCT | 119085–119104 | 799 | US3, US4 | | BamHI-16/17-799-R | AAGACGAGCACGACGATGTA | 119883–119864 | | BamHI-19/20-519-F | CGCCGTTCTACATCACCAC | 126073–126091 | 519 | US2 | | BamHI-19/20-519-R | ATCCTGCCGTCTAGGAGATG | 126572–126591 |
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*Complete SuHV-1 genome (GenBank access: BK001744).
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