Chromatogram of 2-amino-1-(4-nitrophenyl)propane-1,3-diol (1) and chloramphenicol (2), 0.05 mg/mL of each. Mobile phase: sodium pentanesulfonate solution, acetonitrile, glacial acetic acid (85 : 15 : 1, v/v), flow rate 2.0 mL/min, injection volume 10 μL. Column: reversed phase C18, 5 μm, 15 cm length, 4.6 mm inner diameter, UV detection: 278 nm. Peak asymmetry and theoretical plates of 2-amino-1-(4-nitrophenyl)propane-1,3-diol are 1.36, and 3287, respectively. Peak asymmetry, theoretical plates, and resolution of chloramphenicol are 1.25, 5781, and 11.3, respectively.