Research Article
Separation and Quantification of Eight Antidiabetic Drugs on A High-Performance Liquid Chromatography: Its Application to Human Plasma Assay
Table 1
Results of the linearity, LLOQ, and system suitability parameters ().
| | Compound | | LLOQ (ng/mL) | | | | | | | | HETP (mm) |
| | ROS | 245 | 24 | 0.9983 | 3.14 | 2.31 | — | 1.17 | 0.2876 | 9945 | 0.0101 | | PIO | 265 | 36 | 0.9981 | 4.58 | 4.59 | 2.42 | 1.09 | 0.2912 | 9233 | 0.0108 | | GLP | 270 | 35 | 0.9989 | 7.46 | 2.17 | 5.52 | 1.34 | 0.2764 | 10637 | 0.0094 | | GLC | 225 | 30 | 0.9990 | 8.95 | 2.72 | 3.14 | 1.27 | 0.2819 | 7425 | 0.0135 | | REP | 245 | 20 | 0.9987 | 12.17 | 3.11 | 7.01 | 1.25 | 0.3011 | 9910 | 0.0101 | | NGL | 231 | 20 | 0.9989 | 14.39 | 2.79 | 3.47 | 1.42 | 0.3082 | 8941 | 0.0112 | | GLB | 228 | 40 | 0.9978 | 16.32 | 2.09 | 7.46 | 1.37 | 0.2941 | 10003 | 0.0099 | | GLM | 246 | 38 | 0.9987 | 18.78 | 2.57 | 2.89 | 1.28 | 0.2659 | 9081 | 0.0110 |
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: retention time; : capacity factor; : resolution; : tailing factor; : width of peak at 5%; N: theoretical plates (USP); HETP: height equivalent to theoretical plate Length of the column (mm)/theoretical plates.
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