Research Article
Separation and Quantification of Eight Antidiabetic Drugs on A High-Performance Liquid Chromatography: Its Application to Human Plasma Assay
Table 3
Stability data for anti-diabetic drugs in plasma.
| | Drug | Nominal concentration | Sample conditions () | | ng/mL | Short-term | Long-term | Freeze-thaw | | | Nominal (%) | RSD | Nominal (%) | RSD | Nominal (%) | RSD |
| | ROS | 53.7 | 103.1 | 6.2 | 97.5 | 8.6 | 102.4 | 7.1 | | 1531.0 | 99.3 | 2.0 | 98.0 | 2.2 | 98.7 | 1.7 |
| | PIO | 48.9 | 102.0 | 5.9 | 97.1 | 8.8 | 100.6 | 5.9 | | 1513.8 | 99.0 | 2.0 | 97.2 | 1.9 | 99.1 | 1.8 |
| | GLP | 50.6 | 98.9 | 7.6 | 95.6 | 8.0 | 97.1 | 6.6 | | 1498.5 | 99.3 | 1.5 | 98.7 | 1.4 | 99.8 | 0.9 |
| | GLC | 46.8 | 105.7 | 8.7 | 99.6 | 3.4 | 100.1 | 2.3 | | 1467.0 | 97.8 | 2.0 | 97.3 | 4.1 | 98.1 | 4.5 |
| | REP | 49.2 | 107.8 | 8.9 | 99.2 | 3.8 | 99.2 | 3.0 | | 1502.7 | 103.5 | 6.1 | 98.1 | 0.8 | 98.6 | 1.4 |
| | NGL | 52.9 | 98.9 | 11.1 | 96.7 | 7.2 | 96.9 | 5.3 | | 1439.6 | 108.0 | 6.4 | 99.0 | 4.8 | 98.5 | 2.8 |
| | GLB | 45.7 | 95.4 | 13.2 | 95.1 | 10.6 | 95.5 | 7.8 | | 1452.0 | 97.8 | 10.1 | 96.8 | 6.8 | 96.2 | 5.9 |
| | GLM | 48.6 | 103.7 | 11.6 | 99.1 | 1.9 | 98.7 | 2.2 | | 1477.5 | 106.3 | 6.0 | 98.7 | 2.3 | 98.6 | 1.0 |
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Exposed at room temperature for 24 h.
Stored at −70°C.
After three freeze-thaw cycles.
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