The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
Table 1
(a) Clinical features of pancreatic cancer patients.
No
Gender
Age (years)
UICC-stage
Tumor size (mm)
TP (g/dL)
ALB (g/dL)
Che (U/L)
T-Cho (mg/dL)
1
M
66
III
35
6.7
4.3
270
220
2
M
66
III
39
6.6
4.4
359
198
3
M
79
IV
10
6.2
3.8
179
155
4
M
71
III
18
6.8
4.0
207
210
5
M
65
III
35
7.0
3.7
189
131
6
M
78
III
50
6.6
4.4
359
198
7
M
66
IIA
30
6.3
4.1
267
176
8
M
62
IV
10
5.9
3.6
171
169
9
M
38
IA
10
6.7
4.0
289
179
10
M
50
IB
30
7.3
4.6
408
203
11
M
63
IIA
18
6.6
3.9
260
138
12
M
62
IIA
38
6.0
3.4
124
149
13
M
54
IIA
24
7.0
4.5
198
162
14
M
73
IIA
25
6.7
4.1
242
176
15
F
76
IIA
26
5.0
3.3
162
95
16
M
63
IIB
15
7.1
4.2
175
163
17
M
65
IIB
32
5.8
3.5
227
113
18
M
68
IIB
80
6.8
4.0
316
217
19
M
71
IIB
24
7.5
4.1
221
144
20
M
74
IIB
27
7.0
4.3
319
178
21
F
68
IIB
27
6.5
4.0
223
177
22
M
63
IIB
26
6.9
4.3
262
219
23
F
68
IIB
27
6.1
3.5
339
163
24
M
61
IIB
30
6.2
4.0
200
176
25
F
74
IIB
40
6.6
3.8
221
170
26
M
62
IIB
60
8.3
3.5
140
155
27
F
73
IIB
35
5.9
3.2
130
169
28
F
59
IIB
18
7.2
4.4
356
130
29
M
73
IIB
50
5.7
3.3
127
135
30
F
62
IIB
25
6.8
4.1
255
293
31
F
71
III
25
6.8
4.2
294
159
32
F
78
III
50
5.9
3.3
197
182
Ave ± SD
UICC: international union against cancer, M: male, F: female. TP: total protein. ALB: albumin. Che: cholinesterase. T-Cho: total cholesterol. Ave: average. SD: standard deviation. From number 1 to 4 were used for 2-DE, from number 1 to 8 were for first western blot, from number 9 to 32 were for second western blot.
(b) Clinical features of healthy controls.
No
Gender
Age (years)
TP (g/dL)
ALB (g/dL)
Che (U/L)
T-Cho (mg/dL)
1
M
62
7.5
4.7
401
203
2
M
61
7.7
5.1
396
269
3
M
64
7.2
4.4
284
229
4
M
73
7.6
4.7
216
203
5
M
57
8.3
5.0
430
301
6
M
57
6.7
4.8
328
253
7
M
65
7.2
4.8
375
230
8
M
64
7.2
4.6
327
296
9
M
55
6.9
4.6
300
211
10
M
71
7.1
4.7
290
267
11
F
64
8.1
5.4
293
192
12
M
71
7.2
4.5
233
213
13
M
55
7.2
4.5
365
227
14
M
68
7.4
4.4
300
255
15
F
67
7.0
4.5
279
172
16
F
71
7.0
4.5
297
220
17
M
60
6.7
4.2
284
183
18
M
61
7.0
4.7
260
198
19
M
55
6.4
3.9
304
176
20
F
70
7.6
4.7
398
225
21
M
70
7.3
4.6
257
250
22
F
67
7.4
4.5
304
194
23
M
60
7.0
4.4
338
234
24
F
77
8.1
5.0
416
274
25
F
62
7.5
4.6
284
169
26
F
64
7.2
4.5
280
208
27
M
65
7.4
4.6
264
231
28
M
61
7.8
4.3
319
239
29
M
61
7.3
4.6
309
271
30
M
66
7.5
5.0
375
278
31
F
65
7.6
4.5
339
288
32
M
73
7.1
4.4
306
211
Ave ± SD
M: male, F: female. TP: total protein. ALB: albumin. Che: cholinesterase. T-Cho: total cholesterol. Ave: average. SD: standard deviation. From number 1 to 4 were used for 2-0 E, from number 1 to 8 were for first western blot, from number 9 to 32 were for second western blot.