Blow Flies Were One of the Possible Candidates for Transmission of Highly Pathogenic H5N1 Avian Influenza Virus during the 2004 Outbreaks in Japan
Table 2
Detection of A/H5 influenza virus gene from blow flies, Calliphora nigribarbis, collected during 2004–2006.
Fly collection
No. tested
No. pools
No. positive fly pools
Sites*
Date
HA
HA nested
M
1. Ato Town, Yamaguchi
29. Oct. 2004
100
5
0
0
0
1. Ato Town, Yamaguchi
25. Oct. 2005
60
3
0
0
0
2. Yamaguchi City, Yamaguchi
30. Oct. 2005
18
1
0
0
0
3. Fukuoka City, Fukuoka
31. Oct. 2005
100
5
0
0
0
3. Fukuoka City, Fukuoka
28–30. Nov. 2006
340
17
0
0
0
4. Nishiarita Town, Saga
28–30. Nov. 2006
620
31
0
0
1
4. Nishiarita Town, Saga
7. Feb. 2007
319
16
0
0
0
5. Sadohara Town, Miyazaki
26. Jan. 2007
256
13
0
0
0
6. Kojo Town, Miyazaki
27. Jan. 2007
24
2
0
0
0
7. Kiyotake Town, Miyazaki
27. Jan. 2007
50
3
0
0
0
Total
1,887
96
0
0
1
Crops and guts dissected from twenty flies were pooled and tested for the detection of HA and M gene fragments by using RT-PCR and following nested PCR.
*See Figure 4 for more information of each site.
