The Role of Wild-Type p53 in Cisplatin-Induced Chk2 Phosphorylation and the Inhibition of Platinum Resistance with a Chk2 Inhibitor
Figure 1
Cisplatin-induced DNA damage response in ovarian cancer A2780 cells. A2780 cells were treated with 3 μM cisplatin (CDDP) for 1 hr. Cells were then washed to remove cisplatin and reincubated for indicated hours in a replacement of fresh drug-free media. Cells were harvested, and proteins were extracted, separated on SDS-PAGE gel, transferred onto PVDF membrane, and then probed with antibodies of anti-ATM, anti-p-p53 (Ser15 & Ser20), anti-p53, anti-p-Chk2 (Thr68), anti-Chk2, anti-P48, anti-P21 or anti-β-Actin. β-Actin served as a control.
