Raf-1 Activation Prevents Caspase 9 Processing Downstream of Apoptosome Formation
Figure 6
Raf-1 activation prevents cytochrome c/ATP-mediated activation of caspase 9. CCL39-Raf-1:ER cells were deprived of serum for 9 hours in the presence or absence of 4-HT. The cells were lysed in hypotonic buffer and the cell lysates were incubated with 1 M cytochrome c and 1 mM ATP for the indicated times. The cell lysates were analyzed by immunoblotting for caspase-9, phospho-, and total MAPK1,3.
