Research Article

Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli

Figure 4

Digestion by factor Xa using the modified method. M: molecular size markers, 1: TF-tagged LDH, 2: digestion by 0.1 U, 3: digestion by 1 U, 4: digestion by 5 U. To avoid salting-out under the cleavage conditions shown in Figure 3, the reaction solution was diluted by 10-fold with distilled water.
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