Chromatography Research International
Volume 2011 (2011), Article ID 860168, 8 pages
Research Article

LC and UV Methods for Lamotrigine Determination in Pharmaceutical Formulation

Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avendia Ipiranga, 2752/402, 90610-000 Porto Alegre, RS, Brazil

Received 12 July 2010; Revised 3 December 2010; Accepted 14 December 2010

Academic Editor: Toyohide Takeuchi

Copyright © 2011 Magda T. Martins et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Liquid chromatography (LC) and ultraviolet spectrophotometric (UV) methods for lamotrigine (LTG) determination were validated. The LC separation was achieved on an ACE RP-18 as stationary phase and 0.3% triethylamine in water (v/v) pH 4.0 : methanol (62 : 38, v/v) as mobile phase. Detection was achieved with a photodiode array at 279 nm. The detection response for LTG was linear ( 𝑟 2 = 0 . 9 9 9 9 ). The specificity and stability were proved using stress conditions. The CV (%) values for intraday and interday precision were less than 2.0%. The method was accurate and robust. The 𝑡 -student test proved that the LC and UV methods are interchangeable.