Inhibition of cell growth by stable transfection of pSilencer3.1-neo-mix vectors. (a) Anchorage-dependent cell growth was determined by MTT assay. HepG2 cells transfected with pSilencer3.1-neo-mix constructs inhibits proliferation. The x-axis indicates cultured time period, and the y-axis represents the row OD value. Growth rate of cells transfected with control vector (pSilencer3.1-neo-Y) or untransfected cells was compared. (b) Anchorage-independent cell growth was measured by the soft agar assay. Data are presented as means ± SEM.