Antiproliferative, Ultrastructural, and Physiological Effects of Amiodarone on Promastigote and Amastigote Forms of Leishmania amazonensis
Figure 5
Effects of the AMIO on the mitochondrial function of Leishmania amazonensis promastigotes. (a) Values of ΔΨm were evaluated during 30 min, after which the uncoupler FCCP was added to collapse mitochondrial potential. Cells grown in the presence of two concentrations of amiodarone, 6 and 15 μM, for 48 h (curves b and c, resp.) were observed. (b) Effect of oligomycin (10 μM) and FCCP (1 μM) in the ΔΨm was also evaluated. (c) Production of ROS was measured in the same cells. Control and treated cells were incubated with H2DCFDA, and the fluorescence intensity was measured in a Microplate Reader. Fluorescence intensity is expressed as arbitrary units (A.U.). The experiments were performed three times, each time in triplicate, and the figures shown are representative of these experiments.
