Ultrathin sections of L. amazonensis promastigotes treated with different concentrations of AMIO showing several alterations in the mitochondrial ultrastructure such as marked swelling (a, c, d, and e) with loss of the matrix content (a), alterations in the mitochondrial membrane (arrowheads), and presence of autophagic structures near a modified mitochondrion (b). A: autophagic structure; F: flagellum; k: kinetoplast; M: mitochondrion, N: nucleus.