Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Parameter</td><td align="center">SD<sup>b</sup> of peak area</td><td align="center">% R.S.D.<sup>b</sup></td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">Mobile phase composition</td><td align="center">31.35</td><td align="center">1.05</td></tr><tr><td align="left">Amount of mobile phase</td><td align="center">34.83</td><td align="center">1.14</td></tr><tr><td align="left">Temperature</td><td align="center">38.21</td><td align="center">1.25</td></tr><tr><td align="left">Relative humidity</td><td align="center">36.26</td><td align="center">1.19</td></tr><tr><td align="left">Development distance</td><td align="center">32.28</td><td align="center">1.07</td></tr><tr><td align="left">Time from spotting to chromatography</td><td align="center">32.45</td><td align="center">1.07</td></tr><tr><td align="left">Time from chromatography to scanning</td><td align="center">20.85</td><td align="center">0.70</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><svg height="11.625" id="M21" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 40.575001 11.625" width="40.575001" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.625)">
<g transform="translate(72,-62.7)">
<text transform="matrix(1,0,0,-1,-71.95,67.92)">
<tspan style="font-size: 8.75px; " x="0" y="0">a</tspan>
</text>
<text transform="matrix(1,0,0,-1,-67.57,62.92)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑛</tspan>
<tspan style="font-size: 12.50px; " x="9.6898251" y="0">=</tspan>
<tspan style="font-size: 12.50px; " x="21.71771" y="0">6</tspan>
</text>
</g>
</g>
</svg>, <svg height="13.85" id="M22" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 6.2125001 13.85" width="6.2125001" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.85)">
<g transform="translate(72,-60.92)">
<text transform="matrix(1,0,0,-1,-71.95,65.97)">
<tspan style="font-size: 8.75px; " x="0" y="0">b</tspan>
</text>
</g>
</g>
</svg>Average of one concentration: 800 ng per spot.<br/></td></tr></table>

Chromatography Research International

tab3

Table 3

Table 3: Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form 