Journal of Amino Acids

Research Article

Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis

Table 4

Yield of Pacific cod trypsin-catalyzed peptide synthesis.
Entry no.Acyl donor (no.)　　　　Reaction time (hr) Product (No.) Yield (%)
1N-Boc-L-Ala-OmAm (1a)48 N-Boc-L-Ala-L-Ala-pNA (13a)44
2N-Boc-L-Ala-OmGu (2a)24 N-Boc-L-Ala-L-Ala-pNA (13a)37
3N-Boc-L-Ala-OmAM (3a)24 N-Boc-L-Ala-L-Ala-pNA (13a)55
4N-Boc-L-Ala-OmGM (4a)12 N-Boc-L-Ala-L-Ala-pNA (13a)51
5N-Boc-L-Ala-OpAm (5a)0.2 N-Boc-L-Ala-L-Ala-pNA (13a)58
6N-Boc-L-Ala-OpGu (6a)0.2 N-Boc-L-Ala-L-Ala-pNA (13a)60
7N-Boc-L-Ala-OpAM (7a)5 N-Boc-L-Ala-L-Ala-pNA (13a)59
8N-Boc-L-Ala-OpGM (8a)5 N-Boc-L-Ala-L-Ala-pNA (13a)59
9N-Boc-L-Ala-O(1-4)GN (9a)5 N-Boc-L-Ala-L-Ala-pNA (13a)56
10N-Boc-L-Ala-O(1-5)GN (10a)5 N-Boc-L-Ala-L-Ala-pNA (13a)55
11N-Boc-L-Ala-O(1-6)GN (11a)24 N-Boc-L-Ala-L-Ala-pNA (13a)52
12N-Boc-L-Ala-O(2-5)GN (12a)50 N-Boc-L-Ala-L-Ala-pNA (13a)59
13N-Boc-D-Ala-OmAm (1b)74 N-Boc-D-Ala-L-Ala-pNA (13b)12
14N-Boc-D-Ala-OmGm (2b)74 N-Boc-D-Ala-L-Ala-pNA (13b)52
15 -Boc-D-Ala-OmAM (3b)74 N-Boc-D-Ala-L-Ala-pNA (13b)38
16N-Boc-D-Ala-OmGM (4b)74 N-Boc-D-Ala-L-Ala-pNA (13b)58
17N-Boc-D-Ala-OpAm (5b)0.2 N-Boc-D-Ala-L-Ala-pNA (13b)70
18N-Boc-D-Ala-OpGu (6b)0.2 N-Boc-D-Ala-L-Ala-pNA (13b)75
19N-Boc-D-Ala-OpAM (7b)8 N-Boc-D-Ala-L-Ala-pNA (13b)76
20N-Boc-D-Ala-OpGM (8b)48 N-Boc-D-Ala-L-Ala-pNA (13b)74
21N-Boc-D-Ala-O(1-4)GN (9b)5 N-Boc-D-Ala-L-Ala-pNA (13b)57
22N-Boc-D-Ala-O(1-5)GN (10b)97 N-Boc-D-Ala-L-Ala-pNA (13b)61
23N-Boc-D-Ala-O(1-6)GN (11b)97 N-Boc-D-Ala-L-Ala-pNA (13b)33
24N-Boc-D-Ala-O(2-5)GN (12b)74 N-Boc-D-Ala-L-Ala-pNA (13b)23
1 mM acyl donor, 20 mM acyl acceptor, 10 μM PcT, respectively, in MOPS (50 mM, pH 8.0, containing 20 mM CaCl2) : DMSO = 1 : 1, at 25°C.