Parsimony analysis of sequence haplotypes derived from reference Neotyphodium and Epichloë isolates for the EST-SSR locus NCESTA1AB04. (a) One of three most parsimonious trees obtained. The left edge is the inferred midpoint root. Branches with bootstrap values of greater than 70% from 1000 bootstrap replications are marked. The number of isolates with an identical haplotype is indicated in the brackets following the species name. In the instances when multiple haplotypes were identified, the relevant variant number is indicated by the number of asterisks following the species name or isolate number. The boxed area indicated by the dotted line indicates the regions representing subtrees. ((b) and (c)) Subtrees showing the alternative branching of E. sylvatica and E. baconii in the other trees.