Establishment of Methylation-Specific PCR for the Mouse p53 Gene

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left"></td><td align="center"></td><td align="center">Primer sequence (5′–3′)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">U<sup>1</sup></td><td align="center">F<sup>3</sup></td><td align="center">ATC GTT ATT CGG TTT GTT TTC</td></tr><tr><td align="left"></td><td align="center">R<sup>4</sup></td><td align="center">CGA ACA CGA CTC CCA ACT AA</td></tr><tr><td align="left">M<sup>2</sup></td><td align="center">F</td><td align="center">ATC GTT ATT CGG TTT GTT TTC</td></tr><tr><td align="left"></td><td align="center">R</td><td align="center">CGA ACA CGA CTC CCA GCT AA</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><sup>1</sup>U: un-methylated sequence; <sup>2 </sup>M: methylated sequence; <sup>3</sup>F: forward sequence; <sup>4</sup>R: reverse sequence.<br/></td></tr></table>

Molecular Biology International

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Table 1

Table 1: Establishment of Methylation-Specific PCR for the Mouse p53 Gene 