Research Article
HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa
Table 2
Quantification of RT-F2 compound.
| Stationary phase | Precoated Silica Gel 60 GF254 | Mobile phase | Chloroform : toluene : ethyl acetate (6 : 3 : 1) | Calibration range of F2 | 2.5–12.5 μg/spot | Detection | Anisaldehyde sulphuric acid reagent heated at 110°C for 5 min and detected at 600 nm. | Regression equation | (area wise) | value | 0.99862 (area wise) |
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