Journal of Biophysics

Research Article

pH-Dependent Interaction between C-Peptide and Phospholipid Bicelles

Table 1

Diffusion coefficients for the C-peptide.
pHDiffusion coefficient ( 1 0 − 1 0   m 2 /s ) a
BufferDMPC/DHPC(DMPC/DMPG 4: 1)/DHPC
𝐷 f r e e 𝐷 m i x t u r e 𝐷 D M P C 𝑥 (% ) b 𝐷 m i x t u r e 𝐷 D M P C 𝑥 (% ) b
3.2 1 . 5 ± 0 . 1 0 . 8 ± 0 . 1 0 . 4 8 ± 0 . 0 5 69 0 . 7 7 ± 0 . 1 0 . 3 6 ± 0 . 0 5 64
5.8 1 . 6 ± 0 . 1 1 . 4 ± 0 . 1 0 . 4 3 ± 0 . 0 5 17 1 . 1 ± 0 . 1 0 . 4 2 ± 0 . 0 5 34
7.2 1 . 5 ± 0 . 1 1 . 1 ± 0 . 1 0 . 3 4 ± 0 . 0 5 34 1 . 4 ± 0 . 1 0 . 2 5 ± 0 . 0 5 8
a T he diffusion coefficients are normalized according to the diffusion of HDO to account for viscosity differences. b E stimation of the percentage of peptide bound to the phospholipid bicelle as calculated by (1).