Organic Chemistry International
Volume 2012 (2012), Article ID 206025, 6 pages
Research Article

Complexation of Copper(II) with Humic Acids Studied by Ultrasound Spectrometry

Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

Received 23 March 2012; Accepted 14 May 2012

Academic Editor: Dipakranjan Mal

Copyright © 2012 Martina Klučáková. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Copper biogeochemistry is controlled by bonding to natural organic matter. The soluble forms of bonded copper can be more biologically active due to the higher migration in environmental systems and instability of some copper-humic complexes. In this work, the interactions of copper(II) ions with humic acids are studied by means of high-resolution ultrasound spectrometry. It was found that the stoichiometry of the formed complexes is strongly influenced by the organization of humic acid in solution and by the final pH value in equilibrium. Although the ratio between the added copper and humic acids in all used concentrations was constant and the initial pH value was neutral, we observed significant differences between the individual systems. The highest binding ability was determined for a humic content of 0.5 g·dm−3. More diluted and more concentrated systems were able to bind lower amounts of copper. The implemented method is very sensitive and can be utilized not only for monitoring copper binding but also as an indicator of conformational changes of humic acid in solutions with varying concentration.