Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method
Table 4
Results from the robustness study of the method.
Method
Deliberate changes
%RSD of peak area
parameter/condition
MET
OLM
Plate activation time(a)
20 min
1.24
1.01
30 min
0.43
0.36
Chamber saturation time(a)
16 min
1.64
1.71
24 min
0.86
0.80
Volume of mobile phase(b)
8.2 mL
1.22
1.40
10.0 mL
0.39
0.46
Development distance from spot application(c)
7.7 cm
0.31
0.22
6.3 cm
0.42
0.56
(a)±20% change in set time.
(b)±10% change in set volume.
(c)±10% change in set distance.
