Cathepsin D Expression in Colorectal Cancer: From Proteomic Discovery through Validation Using Western Blotting, Immunohistochemistry, and Tissue Microarrays
Figure 1
(a) Spots of cathepsin D from samples of the main tumor body (MTB), IF area (IF), and liver metastasis (LM) on 2 D gels; (b) validation of cathepsin D profiled by 2DE western blotting of cy5 labeled LMD sample (red) and using Cy3 label antibody (green) (ECL-Plex).