Research Article
Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form
Table 3
Robustness evaluation of the method (
).
| | Factor | Level | Retention time () | Asymmetry (T) | | PCM | CHZ | NIM | PCM | CHZ
| NIM |
| | (A) Flow rate (mL min−1) |
| | 1.1 | −1 | 2.700 | 4.625 | 9.582 | 1.24 | 1.07 | 1.12 | | 1.2 | 0 | 2.702 | 4.612 | 9.545 | 1.18 | 1.05 | 1.09 | | 1.3 | +1 | 2.697 | 4.595 | 9.461 | 1.15 | 1.02 | 1.08 |
| Mean ± S.D
| | 2.699 ± 0.04
| 4.610 ± 0.02
| 9.529 ± 0.06
| 1.19 ± 0.04
| 1.05 ± 0.02 | 1.10 ± 0.06 |
| | (B) Percentage of acetonitrile in the mobile phase (v/v) |
| | 44 | −1 | 2.710 | 4.650 | 9.567 | 1.14 | 1.00 | 1.11 | | 45 | 0 | 2.702 | 4.612 | 9.545 | 1.18 | 1.05 | 1.09 | | 46 | +1 | 2.690 | 4.596 | 9.525 | 1.10 | 1.02 | 1.08 |
| | Mean ± S.D | | 2.700 ± 0.01 | 4.619 ± 0.03 | 9.546 ± 0.02
| 1.13 ± 0.04 | 1.03 ± 0.03 | 1.09 ± 0.02 |
| | (C) Columns from different manufacturers |
| | Hypersil GOLD C18 | | 2.702 | 4.612 | 9.545 | 1.18 | 1.05 | 1.09 | | HiQ-Sil HS C18 | | 2.697 | 4.620
| 9.550 | 1.12 | 1.18 | 1.08 |
| Mean ± S.D
| | 2.699 ± 0.03
| 4.616 ± 0.01
| 9.547 ± 0.01
| 1.19 ± 0.03
| 1.05 ± 0.08 | 1.10 ± 0.02 |
|
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