Research Article
Fluorescence Rejection by Shifted Excitation Raman Difference Spectroscopy at Multiple Wavelengths for the Investigation of Biological Samples
Table 2
Raman bands identified in the SERDS spectra of fat tissue from beef and pork and their vibrational assignment.
| Vibrational assignment | 783 nm excitation | 671 nm excitation | 488 nm excitation | Literature value | [References] |
| Quartz | 492 | 494 | 490 | | Quartz | 605 | 606 | 607 | | =C–H in-plane deformation | 726 | 723 | — | 727 [23] | C–C stretch, CH3 rock, C–O stretch | 842 | 843 | 843 | 800–920 [24] | C–C stretch, CH3 rock, C–O stretch | 868 | 869 | 872 | 800–920 [24] | C–C stretch, CH3 rock, C–O stretch | 890 | 886 | 887 | 800–920 [24] | C–C stretch, CH3 rock, C–O stretch | 921 | 919 | 920 | 800–920 [24] | Carotenoid | — | — | 960 | 960–965 [20] | =C–H out of plane bend cis | 970 | 969 | 970 | 970–972 [23, 25, 26] | Carotenoid | — | — | 1003 | 1000–1014 [21] | C–C aliphatic out-of-phase stretch | 1061 | 1060 | 1060 | 1060–1068 [25, 26] | C–C aliphatic stretch | 1084 | 1080 | — | 1076–1090 [23, 24, 26] | C–C aliphatic in phase stretch | 1126 | 1125 | 1125 | 1119–1129 [23, 25] | Carotenoid | — | — | 1153 | 1151–1172 [21] | Carotenoid | — | — | 1188 | 1191–1193 [20] | Carotenoid | — | — | 1211 | 1213–1215 [20] | =C–H symmetric rock cis | 1262 | 1262 | 1263 | 1263–1266 [25, 27] | >CH2 in phase twist | 1297 | 1297 | 1294 | 1295–1305 [24, 26] | CH3 symmetric deformation | 1368 | 1368 | 1366 | 1368 [25] | >CH2 symmetric deformation | 1436 | 1436 | 1436 | 1436–1443 [25, 28, 29] | CH3 antisymmetric deformation | 1457 | 1459 | 1461 | 1455–1460 [25, 29] | Carotenoid | — | — | 1521 | 1511–1535 [21] | C=C cis stretch | 1651 | 1650 | 1653 | 1650–1670 [26] | Carbonyl C=O stretch | 1740 | 1740 | 1738 | 1730–1750 [24] |
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