Research Article
Fluorometric Determination of Drugs Containing α-Methylene Sulfoxide Functional Groups Using - Methylnicotinamide Chloride as a Fluorogenic Agent
Table 5
Recovery data of 1–5 when assayed in spiked human plasma samples using the proposed method.
| | Drug | Claimed drug concentration | Recovered concentration* | % recovery | Mean % recovery ± S.D. | CV |
| | 1 μg/mL | 1
3
12
15
20
30
40
50 | 1.01
2.9
12.02
15.1
19.89
30.15
40.09
49.9 | 101%
97.5%
100.15%
100.6%
99.45%
100.5%
100.3%
99.8% | | 1.05% |
| | 2 ng/mL | 75
200
500
1000
1200 | 73.5
210
500
9700
1224 | 98%
105%
100%
97%
102% | 104±3.21 | 3.20% |
| | 3 ng/mL | 100
300
1200
1500 | 103
295
1204
1498 | 103%
98.3%
100.3%
99.8% | | 1.72% |
| | 4 ng/mL | 100
300
400
600
800
1000
1200
1400 | 101
302
399
603
795
995
1204
1392 | 101%
100.67%
99.75%
100.5%
99.375%
99.5%
100.33%
99.4% | | 0.62% |
| | 5 ng/mL | 50
200
600
800
1000
1500
2100 | 51
196
602
810
1012
1485
2125 | 102%
98%
100.3%
101.25%
101.2%
99%
101.2% | 100.42±43 | 1.43% |
|
|
*Average of triplicate analyses for each concentration.
|
