Research Article
Fluorometric Determination of Drugs Containing α-Methylene Sulfoxide Functional Groups Using - Methylnicotinamide Chloride as a Fluorogenic Agent
Table 5
Recovery data of 1–5 when assayed in spiked human plasma samples using the proposed method.
| Drug | Claimed drug concentration | Recovered concentration* | % recovery | Mean % recovery ± S.D. | CV |
| 1 μg/mL | 1 3 12 15 20 30 40 50 | 1.01 2.9 12.02 15.1 19.89 30.15 40.09 49.9 | 101% 97.5% 100.15% 100.6% 99.45% 100.5% 100.3% 99.8% | | 1.05% |
| 2 ng/mL | 75 200 500 1000 1200 | 73.5 210 500 9700 1224 | 98% 105% 100% 97% 102% | 104±3.21 | 3.20% |
| 3 ng/mL | 100 300 1200 1500 | 103 295 1204 1498 | 103% 98.3% 100.3% 99.8% | | 1.72% |
| 4 ng/mL | 100 300 400 600 800 1000 1200 1400 | 101 302 399 603 795 995 1204 1392 | 101% 100.67% 99.75% 100.5% 99.375% 99.5% 100.33% 99.4% | | 0.62% |
| 5 ng/mL | 50 200 600 800 1000 1500 2100 | 51 196 602 810 1012 1485 2125 | 102% 98% 100.3% 101.25% 101.2% 99% 101.2% | 100.42±43 | 1.43% |
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*Average of triplicate analyses for each concentration.
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