Inner Membrane Protein YhcB Interacts with RodZ Involved in Cell Shape Maintenance in Escherichia coli
Figure 2
Interaction of YhcB and RodZ detected by BACTH system. (a) Schematic representation of YhcB and its truncated derivatives. Predicted transmembrane domain (TM) and cytoplasmic helical structures (H) are indicated. Thick lines with numbers of amino acid residues show regions cloned into indicated plasmids. (b) Indicated plasmids were cotransformed into JE8471 and resultant transformants were streaked on a LB indicator plate containing X-gal (40 μg/mL) and IPTG (0.5 mM), and incubated at 30°C for one day. Blue colour indicates a positive interaction between each fusion protein. (c) Interaction in each cotransformants was also examined quantitatively by β-gal assay as described in Section 2.
