International Scholarly Research Notices

Research Article

Inner Membrane Protein YhcB Interacts with RodZ Involved in Cell Shape Maintenance in Escherichia coli

Table 2

BACTH analysis of interaction between YhcB and inner membrane proteins.
  𝛽 -gal activitya
T25-plasmidbYhcB-T18T18
T25-MurG 2 6 9 . 6 ± 6 0 . 7 7 1 . 4 ± 5 . 5 { } { } { }
T25-MreB 6 8 . 0 ± 9 . 2 7 3 . 7 ± 9 . 2
T25-MreC 4 7 6 . 6 ± 3 . 0 7 1 . 2 ± 6 . 3
T25-MreD 2 5 4 . 5 ± 5 2 . 4 7 5 . 6 ± 2 2 . 5
RodA-T25 3 7 9 . 7 ± 1 9 . 1 8 2 . 7 ± 1 8 . 6
T25-RodZ 7 0 1 . 1 ± 2 1 1 . 7 8 0 . 6 ± 6 . 1
T25-CydB 8 7 . 3 ± 2 7 . 8 7 0 . 9 ± 1 0 . 9
T25-YciS 8 5 3 . 4 ± 6 8 5 . 6 7 8 . 2 ± 2 . 1
T25 5 9 . 3 ± 2 4 . 4 3 1 . 1 ± 2 1 . 6
T18-plasmidcYhcB-T25T25
T18-RodZ 2 9 4 . 5 ± 1 4 . 2 7 8 . 0 ± 7 . 9
T18-FtsB 8 2 . 7 ± 6 . 3 6 4 . 9 ± 1 4 . 1
YhcB-T18 1 2 3 6 . 8 ± 3 9 8 . 2 5 9 . 3 ± 2 4 . 4
Interaction between YhcB and indicated proteins were quantified by measuring 𝛽 -galactosidase activities of transformants harboring the corresponding plasmids.
aNumbers indicate averages of 𝛽 -galactosidase activity (Miller's unit) with standard deviations. T18 and T25 show vector only.
bPlasmids with T25-CyaA domain appended to N (T25-) or C (-T25) terminus of indicated proteins.
cPlasmids with T18 CyaA domain appended to N (T18-) or C (-T18) terminus of indicated proteins.