RhC Phenotyping, Adsorption/Elution Test, and SSP-PCR: The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors
Figure 1
Specific sequence primer-polymerase chain reaction (SSP-PCR) analysis of RHD 1227A polymorphism. The products of SSP-PCR after separation on 2% agarose gel were shown. The specific product were 348 bp, and the internal control product, 629 bp, based on human growth hormone were amplified. Lane 1 = 100 bp ladder marker, lane 2 = true RhD-negative, and lane 3, 4, 5, and 6 = RHD1227A positive.
