Research Article

CallSim: Evaluation of Base Calls Using Sequencing Simulation

Table 1

Model parameter descriptions.

ParameterDescriptionComments

Prob. of polymerase stall—no incorporation on subsequent flowsValue between 0 and 1
Prob. of no base extension—single base or base within a repeat Value between 0 and 1
DriftRate of signal increase over sequential flowsAccounts for process driven signal drift